Chlorine in PDB 6uol: Y271G Dna Polymerase Beta Substrate Complex with A Templating Cytosine and Incoming Rgtp

Enzymatic activity of Y271G Dna Polymerase Beta Substrate Complex with A Templating Cytosine and Incoming Rgtp

All present enzymatic activity of Y271G Dna Polymerase Beta Substrate Complex with A Templating Cytosine and Incoming Rgtp:
2.7.7.7;

Protein crystallography data

The structure of Y271G Dna Polymerase Beta Substrate Complex with A Templating Cytosine and Incoming Rgtp, PDB code: 6uol was solved by M.R.Smith, B.D.Freudenthal, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	25.09 / 1.94
*Space group*	P 1 2₁ 1
*Cell size a, b, c (Å), α, β, γ (°)*	50.800, 80.400, 55.500, 90.00, 107.90, 90.00
*R / R_free (%)*	18.7 / 25

Other elements in 6uol:

The structure of Y271G Dna Polymerase Beta Substrate Complex with A Templating Cytosine and Incoming Rgtp also contains other interesting chemical elements:

Manganese	(Mn)	5 atoms
Sodium	(Na)	3 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Y271G Dna Polymerase Beta Substrate Complex with A Templating Cytosine and Incoming Rgtp (pdb code 6uol). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Y271G Dna Polymerase Beta Substrate Complex with A Templating Cytosine and Incoming Rgtp, PDB code: 6uol:

Chlorine binding site 1 out of 1 in 6uol

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Chlorine Binding Sites List in 6uol

Chlorine binding site 1 out of 1 in the Y271G Dna Polymerase Beta Substrate Complex with A Templating Cytosine and Incoming Rgtp

Mono view

Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Y271G Dna Polymerase Beta Substrate Complex with A Templating Cytosine and Incoming Rgtp within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Cl408 b:48.2 occ:1.00	N	A:HIS34	3.0	19.9	1.0
	C	A:ALA32	3.2	21.0	1.0
	N	A:ILE33	3.2	16.0	1.0
	CA	A:ALA32	3.4	22.0	1.0
	CB	A:ALA32	3.6	17.7	1.0
	CB	A:HIS34	3.6	22.5	1.0
	O	A:ALA32	3.6	24.6	1.0
	N	A:LYS35	3.7	18.3	1.0
	CA	A:HIS34	3.7	18.9	1.0
	CD2	A:HIS34	3.8	25.8	1.0
	C	A:ILE33	4.0	23.1	1.0
	CG	A:HIS34	4.0	27.3	1.0
	O	A:HOH606	4.1	30.6	1.0
	CA	A:ILE33	4.2	21.6	1.0
	C	A:HIS34	4.2	22.9	1.0
	CG2	A:ILE33	4.6	26.2	1.0
	CG	A:LYS35	4.7	22.0	1.0
	CB	A:LYS35	4.7	17.0	1.0
	CA	A:LYS35	4.8	16.9	1.0
	N	A:ALA32	4.8	17.0	1.0
	CB	A:ILE33	5.0	28.7	1.0

Reference:

M.R.Smith, K.S.Alnajjar, N.M.Hoitsma, J.B.Sweasy, B.D.Freudenthal. Molecular Characterization of Oxidized Ribonucleotide Insertion By Human Dna Polymerase Beta J.Biol.Chem. 2020.
ISSN: ESSN 1083-351X

Page generated: Mon Jul 29 15:56:16 2024

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