Chlorine in PDB 6xrk: GH5-4 Broad Specificity Endoglucanase From An Uncultured Bovine Rumen Ciliate

The structure of GH5-4 Broad Specificity Endoglucanase From An Uncultured Bovine Rumen Ciliate, PDB code: 6xrk was solved by C.A.Bingman, R.W.Smith, E.M.Glasgow, B.G.Fox, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.64 / 1.42
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	72.320, 84.990, 121.320, 90.00, 90.00, 90.00
R / Rfree (%)	14.5 / 16.7

The structure of GH5-4 Broad Specificity Endoglucanase From An Uncultured Bovine Rumen Ciliate also contains other interesting chemical elements:

Magnesium

(Mg)

4 atoms

The binding sites of Chlorine atom in the GH5-4 Broad Specificity Endoglucanase From An Uncultured Bovine Rumen Ciliate (pdb code 6xrk). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the GH5-4 Broad Specificity Endoglucanase From An Uncultured Bovine Rumen Ciliate, PDB code: 6xrk:

Chlorine binding site 1 out of 1 in the GH5-4 Broad Specificity Endoglucanase From An Uncultured Bovine Rumen Ciliate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of GH5-4 Broad Specificity Endoglucanase From An Uncultured Bovine Rumen Ciliate within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl403 b:30.1 occ:1.00 HE21 A:GLN242 2.6 34.9 1.0 O A:HOH546 3.1 28.5 1.0 HG2 A:GLN242 3.3 28.6 1.0 NE2 A:GLN242 3.5 29.1 1.0 HB2 A:ASP241 3.5 31.5 1.0 HG3 A:GLN242 3.9 28.6 1.0 CG A:GLN242 3.9 23.8 1.0 HE22 A:GLN242 4.0 34.9 1.0 CD A:GLN242 4.2 27.0 1.0 CB A:ASP241 4.5 26.2 1.0 H A:ASP241 4.6 29.4 1.0 OD2 A:ASP241 4.8 32.1 1.0 HB3 A:ASP241 4.8 31.5 1.0 O A:HOH935 4.9 39.1 1.0

E.M.Glasgow, E.I.Kemna, C.A.Bingman, N.L.Ing, K.Deng, C.M.Bianchetti, T.E.Takasuka, T.R.Northen, B.G.Fox. A Structural and Kinetic Survey of GH5_4 Endoglucanases Reveals Determinants of Broad Substrate Specificity and Opportunities For Biomass Hydrolysis. J.Biol.Chem. 2020.
ISSN: ESSN 1083-351X
PubMed: 33067356
DOI: 10.1074/JBC.RA120.015328