Chlorine in PDB 6y2k: Crystal Structure of Beta-Galactosidase From the Psychrophilic Marinomonas EF1

All present enzymatic activity of Crystal Structure of Beta-Galactosidase From the Psychrophilic Marinomonas EF1:
3.2.1.23;

The structure of Crystal Structure of Beta-Galactosidase From the Psychrophilic Marinomonas EF1, PDB code: 6y2k was solved by M.Mangiagalli, M.Lapi, S.Maione, M.Orlando, S.Brocca, A.Pesce, A.Barbiroli, S.Pucciarelli, C.Camilloni, M.Lotti, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	116.29 / 1.90
Space group	P 63 2 2
Cell size a, b, c (Å), α, β, γ (°)	133.332, 133.332, 232.574, 90.00, 90.00, 120.00
R / Rfree (%)	16.3 / 18.9

The binding sites of Chlorine atom in the Crystal Structure of Beta-Galactosidase From the Psychrophilic Marinomonas EF1 (pdb code 6y2k). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Beta-Galactosidase From the Psychrophilic Marinomonas EF1, PDB code: 6y2k:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Beta-Galactosidase From the Psychrophilic Marinomonas EF1


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Beta-Galactosidase From the Psychrophilic Marinomonas EF1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl701 b:16.9 occ:1.00 NE1 A:TRP12 3.2 16.6 1.0 N A:TYR8 3.4 15.9 1.0 NH2 A:ARG353 3.5 17.4 1.0 N A:ARG355 3.5 16.3 1.0 NE A:ARG353 3.7 16.8 1.0 O A:ARG355 3.7 16.4 1.0 CD1 A:TYR7 3.7 16.7 1.0 CB A:TRP354 3.8 17.1 1.0 CD1 A:TYR8 4.0 15.8 1.0 CA A:TYR7 4.0 16.3 1.0 CZ A:ARG353 4.0 17.1 1.0 N A:TRP354 4.0 16.4 1.0 CE2 A:TRP12 4.1 16.5 1.0 CB A:TYR8 4.1 15.8 1.0 C A:TYR7 4.2 16.2 1.0 CD1 A:TRP12 4.2 16.8 1.0 CB A:ARG355 4.2 17.0 1.0 CD1 A:TRP354 4.2 17.7 1.0 CA A:TYR8 4.2 15.9 1.0 CA A:ARG355 4.2 16.6 1.0 CA A:TRP354 4.3 16.7 1.0 C A:TRP354 4.3 16.7 1.0 CZ2 A:TRP12 4.3 16.4 1.0 CE1 A:TYR7 4.4 17.1 1.0 CB A:ARG353 4.4 16.0 1.0 O A:CYS6 4.4 16.4 1.0 C A:ARG355 4.4 16.5 1.0 CG A:TRP354 4.4 17.4 1.0 O A:TYR8 4.5 15.4 1.0 CG A:TYR8 4.6 15.8 1.0 C A:TYR8 4.6 15.8 1.0 CG A:TYR7 4.7 16.4 1.0 CB A:TYR7 4.8 16.4 1.0 CD A:ARG353 4.9 16.8 1.0 CG A:ARG353 5.0 16.7 1.0 CE1 A:TYR8 5.0 15.9 1.0 C A:ARG353 5.0 16.0 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Beta-Galactosidase From the Psychrophilic Marinomonas EF1


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Beta-Galactosidase From the Psychrophilic Marinomonas EF1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl702 b:17.8 occ:1.00 O A:HOH866 3.2 21.3 1.0 N A:LEU150 3.3 16.1 1.0 N A:VAL149 3.4 16.1 1.0 C A:ASP147 3.4 15.8 1.0 O A:HOH1358 3.4 28.4 1.0 CA A:ASP147 3.5 15.8 1.0 O A:HOH1654 3.5 26.5 1.0 O A:ASP147 3.6 15.5 1.0 CB A:VAL149 3.6 16.4 1.0 CB A:ALA249 3.6 15.8 1.0 N A:THR148 3.8 15.7 1.0 CB A:ASP147 3.8 16.1 1.0 CA A:VAL149 3.9 16.3 1.0 C A:VAL149 4.1 16.2 1.0 CG A:LEU150 4.1 17.1 1.0 CB A:LEU150 4.1 16.4 1.0 O A:HOH910 4.2 16.6 1.0 CG2 A:VAL149 4.3 16.8 1.0 CA A:LEU150 4.3 16.0 1.0 C A:THR148 4.4 16.0 1.0 CA A:THR148 4.6 15.6 1.0 OD1 A:ASP147 4.6 16.9 1.0 CZ A:PHE247 4.7 17.4 1.0 CG A:ASP147 4.7 17.0 1.0 O A:LEU150 4.7 16.2 1.0 CG1 A:VAL149 4.8 16.9 1.0 CD1 A:LEU150 4.8 17.7 1.0 N A:ASP147 4.8 15.4 1.0 O A:GLY144 4.9 14.9 1.0

M.Mangiagalli, M.Lapi, S.Maione, M.Orlando, S.Brocca, A.Pesce, A.Barbiroli, C.Camilloni, S.Pucciarelli, M.Lotti, M.Nardini. The Co-Existence of Cold Activity and Thermal Stability in An Antarctic GH42 Beta-Galactosidase Relies on Its Hexametric Quaternary Arrangement. Febs J. 2020.
ISSN: ISSN 1742-464X
PubMed: 32363751
DOI: 10.1111/FEBS.15354