Chlorine in PDB 6y71: Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H130A

Enzymatic activity of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H130A

All present enzymatic activity of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H130A:
1.7.2.4;

Protein crystallography data

The structure of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H130A, PDB code: 6y71 was solved by L.Zhang, P.M.H.Kroneck, O.Einsle, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 76.21 / 1.64
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 69.156, 76.213, 109.016, 90, 93.21, 90
R / Rfree (%) 16.9 / 21.1

Other elements in 6y71:

The structure of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H130A also contains other interesting chemical elements:

Calcium (Ca) 2 atoms
Copper (Cu) 4 atoms
Sodium (Na) 2 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H130A (pdb code 6y71). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H130A, PDB code: 6y71:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 6y71

Go back to Chlorine Binding Sites List in 6y71
Chlorine binding site 1 out of 2 in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H130A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H130A within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl706

b:19.7
occ:0.48
ND2 A:ASN241 2.5 24.0 1.0
NH1 A:ARG181 3.1 27.2 1.0
NE2 A:HIS382 3.1 20.7 1.0
N A:CYS328 3.1 18.2 1.0
CD2 A:HIS382 3.2 20.0 1.0
OD1 A:ASN329 3.2 23.5 1.0
O A:CYS328 3.4 20.2 1.0
CG A:ASN241 3.5 26.4 1.0
CB A:ASN241 3.5 16.8 1.0
CA A:GLY327 3.6 14.2 1.0
CG A:ASN329 3.8 21.7 1.0
ND2 A:ASN329 3.8 15.8 1.0
C A:CYS328 3.8 18.7 1.0
C A:GLY327 3.9 17.8 1.0
CZ A:ARG181 4.0 34.7 1.0
CA A:CYS328 4.1 19.9 1.0
NH2 A:ARG181 4.1 51.6 1.0
O A:HOH1132 4.2 34.8 1.0
O A:HOH1189 4.3 16.3 1.0
CE1 A:HIS382 4.4 25.3 1.0
CE1 A:HIS433 4.5 17.6 1.0
CG A:HIS382 4.5 16.9 1.0
OD1 A:ASN241 4.6 27.7 1.0
CB A:CYS328 4.7 20.8 1.0
N A:ASN329 4.7 17.7 1.0
CA A:ASN241 4.9 15.4 1.0
N A:GLY327 4.9 17.1 1.0
O A:THR242 5.0 14.6 1.0

Chlorine binding site 2 out of 2 in 6y71

Go back to Chlorine Binding Sites List in 6y71
Chlorine binding site 2 out of 2 in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H130A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H130A within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl705

b:29.9
occ:0.50
ND2 B:ASN241 2.4 27.1 1.0
NH1 B:ARG181 2.9 42.8 1.0
N B:CYS328 3.1 20.0 1.0
NE2 B:HIS382 3.1 25.8 1.0
CD2 B:HIS382 3.3 21.8 1.0
CG B:ASN241 3.3 27.3 1.0
CB B:ASN241 3.3 17.9 1.0
ND2 B:ASN329 3.4 20.7 1.0
O B:CYS328 3.4 29.7 1.0
CA B:GLY327 3.6 18.3 1.0
O B:HOH992 3.7 37.8 1.0
C B:GLY327 3.8 20.6 1.0
C B:CYS328 3.9 26.0 1.0
CG B:ASN329 4.0 24.1 1.0
OD1 B:ASN329 4.0 21.4 1.0
CA B:CYS328 4.0 23.3 1.0
O B:HOH1133 4.0 17.9 1.0
CZ B:ARG181 4.2 43.0 1.0
CE1 B:HIS382 4.4 27.3 1.0
CE1 B:HIS433 4.4 22.0 1.0
OD1 B:ASN241 4.5 27.4 1.0
O B:HOH1134 4.5 37.6 1.0
CB B:CYS328 4.6 19.2 1.0
CG B:HIS382 4.6 20.5 1.0
CA B:ASN241 4.7 20.7 1.0
N B:GLY327 4.8 21.0 1.0
N B:THR242 4.8 19.9 1.0
N B:ASN329 4.9 20.2 1.0
O B:HIS326 4.9 25.8 1.0
O B:THR242 4.9 19.3 1.0
NH2 B:ARG181 5.0 44.8 1.0
NE2 B:HIS433 5.0 19.6 1.0

Reference:

L.Zhang, E.Bill, P.M.H.Kroneck, O.Einsle. A [3CU:2S] Cluster Provides Insight Into the Assembly and Function of the Cuz Site of Nitrous Oxide Reductase Chem Sci 2021.
ISSN: ESSN 2041-6539
DOI: 10.1039/D0SC05204C
Page generated: Wed Mar 3 12:55:17 2021

Last articles

Zn in 7VD8
Zn in 7V1R
Zn in 7V1Q
Zn in 7VPF
Zn in 7T85
Zn in 7T5F
Zn in 7NF9
Zn in 7M4M
Zn in 7M4O
Zn in 7M4N
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy