Chlorine in PDB 7c5l: Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution

The structure of Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution, PDB code: 7c5l was solved by L.Zhang, M.R.Liu, L.Y.Bao, Y.C.Yao, I.K.Bostrom, Y.D.Wang, A.Q.Chen, J.X.Li, S.H.Gu, C.N.Ji, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.89 / 2.09
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	89.4, 89.4, 340.266, 90, 90, 90
R / Rfree (%)	14.8 / 20.1

The binding sites of Chlorine atom in the Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution (pdb code 7c5l). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 5 binding sites of Chlorine where determined in the Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution, PDB code: 7c5l:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5;

Chlorine binding site 1 out of 5 in the Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution within 5.0Å range: probe atom residue distance (Å) B Occ O:Cl402 b:22.6 occ:1.00 O O:HOH780 3.2 33.3 1.0 O O:HOH533 3.2 16.1 1.0 N O:LYS81 3.3 17.1 1.0 N O:ALA80 3.5 15.7 1.0 CB O:ALA108 3.6 15.5 1.0 CB O:LYS81 3.7 20.7 1.0 CG O:LYS81 3.8 23.4 1.0 C O:GLU79 4.0 17.2 1.0 CB O:GLU79 4.0 19.4 1.0 CE1 O:TYR100 4.0 15.6 1.0 CB O:ALA80 4.1 17.7 1.0 CA O:ALA80 4.1 16.9 1.0 CA O:LYS81 4.1 19.2 1.0 CD O:LYS81 4.2 27.1 1.0 CA O:GLU79 4.2 18.8 1.0 C O:ALA80 4.2 17.1 1.0 OH O:TYR100 4.6 14.4 1.0 CZ O:TYR100 4.8 15.8 1.0 O O:GLU79 4.8 16.4 1.0 CD1 O:TYR100 4.9 15.0 1.0 CG O:GLU79 4.9 22.5 1.0 CE O:LYS81 5.0 32.0 1.0

Chlorine binding site 2 out of 5 in the Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution within 5.0Å range: probe atom residue distance (Å) B Occ O:Cl403 b:25.7 occ:1.00 O O:HOH614 2.8 31.5 1.0 NH1 O:ARG232 3.2 16.4 1.0 NE O:ARG232 3.2 17.3 1.0 CB O:ALA230 3.4 16.1 1.0 CD2 O:HIS177 3.5 16.7 1.0 O O:HOH723 3.5 33.9 1.0 CD2 O:HIS207 3.6 28.2 1.0 CZ O:ARG232 3.7 16.4 1.0 NE2 O:HIS177 3.9 15.8 1.0 CG O:HIS177 3.9 14.8 1.0 CB O:THR209 4.1 21.8 1.0 CD O:ARG232 4.4 17.1 1.0 CB O:HIS177 4.4 14.4 1.0 NE2 O:HIS207 4.4 31.2 1.0 OG1 O:THR151 4.4 17.1 1.0 OG1 O:THR209 4.5 24.4 1.0 ND1 O:HIS177 4.5 16.2 1.0 CE1 O:HIS177 4.5 14.9 1.0 CG2 O:THR175 4.5 15.3 1.0 CG O:HIS207 4.6 25.2 1.0 CG2 O:THR209 4.7 23.0 1.0 O O:THR208 4.8 21.7 1.0 CA O:ALA230 4.9 15.9 1.0 CB O:HIS207 5.0 20.7 1.0

Chlorine binding site 3 out of 5 in the Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution within 5.0Å range: probe atom residue distance (Å) B Occ P:Cl402 b:27.9 occ:1.00 NH1 P:ARG232 3.1 15.4 1.0 O P:HOH643 3.1 28.6 1.0 NE P:ARG232 3.3 14.6 1.0 CD2 P:HIS177 3.3 18.7 1.0 CB P:ALA230 3.5 14.0 1.0 CD2 P:HIS207 3.5 29.9 1.0 CZ P:ARG232 3.7 15.2 1.0 NE2 P:HIS177 3.8 19.4 1.0 CG P:HIS177 3.9 16.6 1.0 CB P:THR209 4.1 21.5 1.0 NE2 P:HIS207 4.2 30.5 1.0 CB P:HIS177 4.4 15.7 1.0 OG1 P:THR151 4.4 16.5 1.0 CG2 P:THR209 4.4 21.3 1.0 CD P:ARG232 4.4 15.4 1.0 CE1 P:HIS177 4.5 17.9 1.0 ND1 P:HIS177 4.5 17.9 1.0 CG2 P:THR175 4.6 17.7 1.0 CG P:HIS207 4.6 28.5 1.0 OG1 P:THR209 4.7 22.3 1.0 O P:THR208 4.8 23.3 1.0 CA P:ALA230 5.0 14.8 1.0 NH2 P:ARG232 5.0 17.1 1.0

Chlorine binding site 4 out of 5 in the Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution within 5.0Å range: probe atom residue distance (Å) B Occ Q:Cl402 b:22.8 occ:1.00 O Q:HOH534 2.8 33.4 1.0 NH1 Q:ARG232 3.2 17.5 1.0 NE Q:ARG232 3.3 15.9 1.0 O Q:HOH601 3.4 32.3 1.0 CB Q:ALA230 3.5 15.9 1.0 CD2 Q:HIS207 3.5 25.0 1.0 CD2 Q:HIS177 3.5 17.4 1.0 CZ Q:ARG232 3.7 16.9 1.0 NE2 Q:HIS177 3.9 16.0 1.0 CG Q:HIS177 4.0 16.1 1.0 CB Q:THR209 4.1 23.4 1.0 NE2 Q:HIS207 4.2 29.2 1.0 OG1 Q:THR151 4.4 13.7 1.0 CB Q:HIS177 4.5 15.7 1.0 CG2 Q:THR209 4.5 23.6 1.0 CD Q:ARG232 4.5 14.5 1.0 ND1 Q:HIS177 4.5 15.7 1.0 CE1 Q:HIS177 4.5 15.2 1.0 O Q:HOH723 4.5 40.2 1.0 CG2 Q:THR175 4.5 15.6 1.0 OG1 Q:THR209 4.6 24.7 1.0 CG Q:HIS207 4.6 24.8 1.0 CA Q:ALA230 4.9 15.1 1.0 O Q:THR208 4.9 22.7 1.0

Chlorine binding site 5 out of 5 in the Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Crystal Structure of C150S Mutant of Glyceraldehyde-3-Phosphate- DEHYDROGENASE1 From Escherichia Coli at 2.1 Angstrom Resolution within 5.0Å range: probe atom residue distance (Å) B Occ R:Cl402 b:30.3 occ:1.00 O R:HOH557 3.0 36.3 1.0 NE R:ARG232 3.2 16.8 1.0 NH1 R:ARG232 3.2 20.5 1.0 CD2 R:HIS177 3.5 19.7 1.0 CB R:ALA230 3.5 18.4 1.0 CD2 R:HIS207 3.6 33.3 1.0 CZ R:ARG232 3.7 17.5 1.0 NE2 R:HIS177 3.9 18.4 1.0 CG R:HIS177 3.9 18.7 1.0 CB R:THR209 4.0 31.0 1.0 NE2 R:HIS207 4.2 34.9 1.0 OG1 R:THR151 4.4 24.1 1.0 CD R:ARG232 4.4 16.9 1.0 OG1 R:THR209 4.4 32.4 1.0 CB R:HIS177 4.4 16.5 1.0 CE1 R:HIS177 4.5 17.1 1.0 CG2 R:THR209 4.5 28.7 1.0 CG2 R:THR175 4.5 17.3 1.0 ND1 R:HIS177 4.5 18.1 1.0 CG R:HIS207 4.7 34.2 1.0 O R:THR208 4.7 30.1 1.0 CA R:ALA230 5.0 17.2 1.0

L.Zhang, M.R.Liu, L.Y.Bao, Y.C.Yao, I.K.Bostrom, Y.D.Wang, A.Q.Chen, J.X.Li, S.H.Gu, C.N.Ji. Crystal Structure of TYPE1 Glyceraldehyde-3-Phosphate Dehydrogenase From Escherichia Coli Provides New Insight Into Bpg Generation and Catalytic Mechanism To Be Published.