Chlorine in PDB 7cvs: Crystal Structure of the C85A/L194A Mutant Clc-EC1 with Fab Fragment

The structure of Crystal Structure of the C85A/L194A Mutant Clc-EC1 with Fab Fragment, PDB code: 7cvs was solved by K.Park, K.Mersch, J.Robertson, H.-H.Lim, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.96 / 3.01
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	232.909, 96.721, 170.731, 90, 131.65, 90
R / Rfree (%)	20.1 / 25.5

The binding sites of Chlorine atom in the Crystal Structure of the C85A/L194A Mutant Clc-EC1 with Fab Fragment (pdb code 7cvs). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Crystal Structure of the C85A/L194A Mutant Clc-EC1 with Fab Fragment, PDB code: 7cvs:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Crystal Structure of the C85A/L194A Mutant Clc-EC1 with Fab Fragment


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the C85A/L194A Mutant Clc-EC1 with Fab Fragment within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl501 b:151.6 occ:1.00 OH A:TYR445 2.6 127.6 1.0 OG A:SER107 2.9 136.1 1.0 N A:ILE356 3.2 102.9 1.0 CA A:GLY355 3.3 102.9 1.0 CB A:SER107 3.4 134.9 1.0 N A:PHE357 3.5 95.5 1.0 CB A:PHE357 3.6 100.2 1.0 CZ A:TYR445 3.7 118.1 1.0 C A:GLY355 3.8 98.9 1.0 CD1 A:ILE109 4.0 87.3 1.0 CE2 A:TYR445 4.0 110.5 1.0 CA A:PHE357 4.2 95.4 1.0 CA A:GLY149 4.2 95.2 1.0 CA A:ILE356 4.2 102.6 1.0 C A:ILE356 4.3 99.1 1.0 CG2 A:ILE356 4.3 96.9 1.0 CA A:SER107 4.3 128.6 1.0 N A:GLY149 4.4 98.9 1.0 CG1 A:ILE109 4.5 87.6 1.0 OE2 A:GLU148 4.5 128.1 1.0 CG A:GLU148 4.5 116.2 1.0 N A:GLY355 4.6 92.4 1.0 CG A:PHE357 4.7 105.5 1.0 CD1 A:PHE357 4.8 111.2 1.0 CB A:ILE356 4.8 96.3 1.0 CE1 A:TYR445 4.8 120.4 1.0 O A:GLY355 5.0 100.7 1.0

Chlorine binding site 2 out of 4 in the Crystal Structure of the C85A/L194A Mutant Clc-EC1 with Fab Fragment


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the C85A/L194A Mutant Clc-EC1 with Fab Fragment within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl502 b:184.3 occ:1.00 N A:SER107 2.7 124.6 1.0 N A:GLY108 3.3 126.0 1.0 CB A:SER107 3.4 134.9 1.0 CA A:SER107 3.4 128.6 1.0 CD A:PRO110 3.5 103.4 1.0 C A:GLY106 3.6 122.0 1.0 CG A:PRO110 3.7 98.7 1.0 CA A:GLY106 3.7 123.7 1.0 C A:SER107 3.8 128.2 1.0 O A:GLY105 3.8 134.4 1.0 CZ A:PHE348 4.4 92.8 1.0 CG2 A:ILE448 4.4 85.8 1.0 CA A:GLY108 4.4 122.0 1.0 OG A:SER107 4.4 136.1 1.0 CB A:PRO110 4.6 96.1 1.0 O A:GLY106 4.6 121.4 1.0 C A:GLY105 4.7 124.1 1.0 N A:GLY106 4.7 127.7 1.0 CD1 A:ILE448 4.7 93.7 1.0 CE1 A:PHE348 4.8 102.3 1.0 N A:PRO110 4.9 93.2 1.0 N A:ILE109 4.9 109.1 1.0

Chlorine binding site 3 out of 4 in the Crystal Structure of the C85A/L194A Mutant Clc-EC1 with Fab Fragment


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of the C85A/L194A Mutant Clc-EC1 with Fab Fragment within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl501 b:249.8 occ:1.00 OH B:TYR445 2.4 137.8 1.0 OG B:SER107 3.0 114.0 1.0 CA B:GLY355 3.3 109.9 1.0 N B:ILE356 3.4 105.4 1.0 CB B:SER107 3.4 121.3 1.0 CZ B:TYR445 3.5 131.5 1.0 CD1 B:ILE109 3.6 96.5 1.0 N B:PHE357 3.7 127.6 1.0 CB B:PHE357 3.9 111.5 1.0 C B:GLY355 3.9 109.7 1.0 CE2 B:TYR445 4.1 126.8 1.0 OE2 B:GLU148 4.2 140.1 1.0 CG B:GLU148 4.2 123.8 1.0 CG1 B:ILE109 4.2 95.7 1.0 CA B:GLY149 4.2 97.9 1.0 CA B:SER107 4.2 124.6 1.0 N B:GLY149 4.4 107.4 1.0 CA B:PHE357 4.4 115.6 1.0 CA B:ILE356 4.5 108.3 1.0 CE1 B:TYR445 4.6 126.1 1.0 C B:ILE356 4.6 122.1 1.0 N B:GLY355 4.6 110.4 1.0 CG2 B:ILE356 4.7 98.1 1.0 CD B:GLU148 4.7 138.1 1.0 CD1 B:PHE357 4.8 119.5 1.0 CG B:PHE357 4.8 116.9 1.0 C B:SER107 5.0 121.6 1.0 CB B:ILE109 5.0 96.0 1.0

Chlorine binding site 4 out of 4 in the Crystal Structure of the C85A/L194A Mutant Clc-EC1 with Fab Fragment


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Crystal Structure of the C85A/L194A Mutant Clc-EC1 with Fab Fragment within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl502 b:233.9 occ:1.00 N B:SER107 3.1 137.7 1.0 CB B:SER107 3.5 121.3 1.0 N B:GLY108 3.7 125.7 1.0 CD B:PRO110 3.7 122.2 1.0 CG B:PRO110 3.7 91.7 1.0 CA B:SER107 3.8 124.6 1.0 C B:GLY106 4.0 133.3 1.0 CZ B:PHE348 4.1 106.9 1.0 CA B:GLY106 4.2 125.4 1.0 CG2 B:ILE448 4.2 114.9 1.0 O B:GLY105 4.2 117.3 1.0 C B:SER107 4.2 121.6 1.0 CE1 B:PHE348 4.4 105.2 1.0 OG B:SER107 4.4 114.0 1.0 CD1 B:ILE448 4.5 87.9 1.0 CB B:PRO110 4.5 93.3 1.0 CA B:GLY108 4.7 119.2 1.0 N B:PRO110 5.0 115.9 1.0

K.Park, K.Mersch, J.Robertson, H.-H.Lim. Crystal Structure of the C85A/L194A Mutant Clc-EC1 with Fab Fragment To Be Published.