Chlorine in PDB 7m08: Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound 1-Nt Gapped Ssb Substrate and Incoming Dumpnpp

Enzymatic activity of Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound 1-Nt Gapped Ssb Substrate and Incoming Dumpnpp

All present enzymatic activity of Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound 1-Nt Gapped Ssb Substrate and Incoming Dumpnpp:
2.7.7.7;

Protein crystallography data

The structure of Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound 1-Nt Gapped Ssb Substrate and Incoming Dumpnpp, PDB code: 7m08 was solved by A.M.Kaminski, K.Bebenek, L.C.Pedersen, T.A.Kunkel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 43.82 / 1.70
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 56.136, 59.862, 140.189, 90, 90, 90
R / Rfree (%) 18.6 / 20.9

Other elements in 7m08:

The structure of Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound 1-Nt Gapped Ssb Substrate and Incoming Dumpnpp also contains other interesting chemical elements:

Sodium (Na) 3 atoms
Magnesium (Mg) 1 atom

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound 1-Nt Gapped Ssb Substrate and Incoming Dumpnpp (pdb code 7m08). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound 1-Nt Gapped Ssb Substrate and Incoming Dumpnpp, PDB code: 7m08:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 7m08

Go back to Chlorine Binding Sites List in 7m08
Chlorine binding site 1 out of 2 in the Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound 1-Nt Gapped Ssb Substrate and Incoming Dumpnpp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound 1-Nt Gapped Ssb Substrate and Incoming Dumpnpp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl604

b:52.4
occ:1.00
N A:GLU556 3.4 26.0 1.0
O A:HOH888 3.6 36.8 1.0
CA A:THR555 3.7 29.7 1.0
O A:HOH781 3.7 42.2 1.0
CB A:THR555 3.8 35.4 1.0
C A:THR555 4.1 27.7 1.0
CG A:GLU556 4.1 24.2 1.0
CD2 A:LEU503 4.1 26.4 1.0
CB A:GLU556 4.4 23.6 1.0
CG2 A:THR555 4.4 33.0 1.0
CA A:GLU556 4.5 25.8 1.0
O A:PRO554 4.6 31.3 1.0
OE1 A:GLU556 4.8 26.9 1.0
CD A:GLU556 4.8 24.4 1.0
CB A:ALA500 4.8 24.8 1.0
N A:THR555 4.9 29.2 1.0

Chlorine binding site 2 out of 2 in 7m08

Go back to Chlorine Binding Sites List in 7m08
Chlorine binding site 2 out of 2 in the Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound 1-Nt Gapped Ssb Substrate and Incoming Dumpnpp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound 1-Nt Gapped Ssb Substrate and Incoming Dumpnpp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl605

b:25.7
occ:0.86
NH2 A:ARG517 3.1 19.1 1.0
N A:GLU529 3.2 19.1 1.0
NH1 A:ARG517 3.2 21.8 1.0
CG A:GLU529 3.3 29.4 1.0
N2 T:DG6 3.3 18.8 1.0
CB A:GLU529 3.5 20.7 1.0
N3 T:DG6 3.5 17.2 1.0
CZ A:ARG517 3.6 20.1 1.0
C2 T:DG6 3.9 16.9 1.0
O4' T:DT7 3.9 22.7 1.0
CA A:GLU529 3.9 19.9 1.0
C4' T:DT7 3.9 25.9 1.0
C1' T:DT7 4.0 26.5 1.0
CD A:GLU529 4.0 39.8 1.0
CD1 A:TYR505 4.1 15.0 1.0
C A:SER528 4.2 21.3 1.0
CB A:SER528 4.2 22.4 1.0
CA A:SER528 4.2 19.5 1.0
CE1 A:TYR505 4.3 14.3 1.0
CG A:TYR505 4.6 14.3 1.0
C5' T:DT7 4.6 39.2 1.0
C4 T:DG6 4.7 17.7 1.0
C1' T:DG6 4.8 19.1 1.0
CZ A:TYR505 4.8 17.6 1.0
NE A:ARG517 4.9 19.0 1.0
C2' T:DT7 4.9 29.7 1.0
N1 T:DT7 5.0 20.8 1.0

Reference:

A.M.Kaminski, K.Bebenek, T.A.Kunkel, L.C.Pedersen. Comprehensive Structural Survey of Dna Double-Strand Break Synapsis By Dna Polymerase Lambda To Be Published.
Page generated: Tue Jul 30 00:18:55 2024

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