Chlorine in PDB 7ovg: The C146A Variant of An Amidase From Pyrococcus Horikoshii with Bound Acetamide

The structure of The C146A Variant of An Amidase From Pyrococcus Horikoshii with Bound Acetamide, PDB code: 7ovg was solved by S.Su, S.Makumire, B.T.Sewell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.85 / 1.65
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	44.979, 57.012, 61.358, 68.09, 85.16, 76.93
R / Rfree (%)	15.1 / 18.2

The binding sites of Chlorine atom in the The C146A Variant of An Amidase From Pyrococcus Horikoshii with Bound Acetamide (pdb code 7ovg). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the The C146A Variant of An Amidase From Pyrococcus Horikoshii with Bound Acetamide, PDB code: 7ovg:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the The C146A Variant of An Amidase From Pyrococcus Horikoshii with Bound Acetamide


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of The C146A Variant of An Amidase From Pyrococcus Horikoshii with Bound Acetamide within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:36.8 occ:1.00 O A:HOH459 3.1 29.1 1.0 N A:MET174 3.5 27.2 1.0 C2 A:ACM301 3.5 34.9 1.0 CE A:MET174 3.5 36.1 1.0 N A:VAL173 3.6 24.3 1.0 CD2 A:LEU172 3.7 30.7 1.0 NE1 A:TRP149 3.7 26.1 1.0 CB A:MET174 3.8 29.6 1.0 CA A:LEU172 3.9 19.1 1.0 CG A:MET174 4.0 31.3 1.0 CB A:LEU172 4.1 20.9 1.0 CA A:MET174 4.1 26.0 1.0 C A:LEU172 4.3 22.1 1.0 O A:MET174 4.4 27.2 1.0 CE2 A:TRP149 4.5 23.7 1.0 CZ2 A:TRP149 4.5 24.4 1.0 CG A:LEU172 4.5 24.1 1.0 C A:VAL173 4.5 27.8 1.0 SD A:MET174 4.6 34.5 1.0 CA A:VAL173 4.6 25.1 1.0 CG2 A:VAL173 4.6 30.2 1.0 C A:MET174 4.6 24.1 1.0 CD1 A:TRP149 4.8 20.2 1.0 C1 A:ACM301 4.9 34.5 1.0

Chlorine binding site 2 out of 2 in the The C146A Variant of An Amidase From Pyrococcus Horikoshii with Bound Acetamide


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of The C146A Variant of An Amidase From Pyrococcus Horikoshii with Bound Acetamide within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl302 b:40.6 occ:1.00 O B:HOH443 3.1 31.0 1.0 C2 B:ACM301 3.5 34.0 1.0 CE B:MET174 3.5 33.8 1.0 N B:MET174 3.5 23.5 1.0 CD2 B:LEU172 3.6 29.9 1.0 N B:VAL173 3.7 23.0 1.0 NE1 B:TRP149 3.7 24.2 1.0 CB B:MET174 3.7 25.8 1.0 CA B:LEU172 3.9 20.3 1.0 CB B:LEU172 4.0 21.5 1.0 CA B:MET174 4.1 23.8 1.0 CG B:MET174 4.2 29.0 1.0 C B:LEU172 4.3 21.8 1.0 O B:MET174 4.4 26.8 1.0 CG B:LEU172 4.5 23.9 1.0 CE2 B:TRP149 4.5 25.0 1.0 CZ2 B:TRP149 4.5 30.9 1.0 C B:VAL173 4.6 25.3 1.0 C B:MET174 4.6 21.6 1.0 SD B:MET174 4.6 31.7 1.0 CA B:VAL173 4.7 22.7 1.0 CG2 B:VAL173 4.7 31.6 1.0 CD1 B:TRP149 4.8 22.1 1.0 C1 B:ACM301 4.9 30.1 1.0

S.Makumire, S.Su, B.W.Weber, J.D.Woodward, S.W.Kimani, R.Hunter, B.T.Sewell. The Structure of the C146A Variant of the Amidase From Pyrococcus Horikoshii Bound to Glutaramide and Acetamide Suggests the Basis of Amide Recognition To Be Published.