Chlorine in PDB 7puj: Crystal Structure of Endoglycosidase E GH18 Domain From Enterococcus Faecalis

All present enzymatic activity of Crystal Structure of Endoglycosidase E GH18 Domain From Enterococcus Faecalis:
3.2.1.52;

The structure of Crystal Structure of Endoglycosidase E GH18 Domain From Enterococcus Faecalis, PDB code: 7puj was solved by M.Garcia-Alija, J.J.Du, B.Trastoy, E.J.Sundberg, M.E.Guerin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.82 / 1.75
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	112.15, 112.15, 64.62, 90, 90, 120
R / Rfree (%)	18.6 / 22

The structure of Crystal Structure of Endoglycosidase E GH18 Domain From Enterococcus Faecalis also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Chlorine atom in the Crystal Structure of Endoglycosidase E GH18 Domain From Enterococcus Faecalis (pdb code 7puj). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Endoglycosidase E GH18 Domain From Enterococcus Faecalis, PDB code: 7puj:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Endoglycosidase E GH18 Domain From Enterococcus Faecalis


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Endoglycosidase E GH18 Domain From Enterococcus Faecalis within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl505 b:27.1 occ:1.00 ZN A:ZN501 2.2 25.2 1.0 OD1 A:ASP215 3.4 27.2 1.0 CL A:CL506 3.5 37.8 1.0 O A:HOH733 3.5 30.3 1.0 CB A:THR214 3.9 22.7 1.0 CG A:ARG216 4.1 27.0 1.0 CD A:ARG216 4.2 28.5 1.0 CG2 A:THR214 4.3 24.6 1.0 CG A:ASP215 4.5 24.1 1.0 N A:ASP215 4.6 23.1 1.0 OG1 A:THR214 4.7 25.0 1.0 CA A:THR214 4.7 24.5 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Endoglycosidase E GH18 Domain From Enterococcus Faecalis


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Endoglycosidase E GH18 Domain From Enterococcus Faecalis within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl506 b:37.8 occ:1.00 ZN A:ZN501 2.2 25.2 1.0 O A:HOH751 3.0 34.7 1.0 OD1 A:ASP215 3.2 27.2 1.0 CG A:ARG216 3.5 27.0 1.0 CL A:CL505 3.5 27.1 1.0 CG A:ASP215 4.1 24.1 1.0 CD A:ARG216 4.2 28.5 1.0 OD1 A:ASP179 4.3 29.4 1.0 OD2 A:ASP215 4.3 23.3 1.0 NE A:ARG216 4.6 21.1 1.0 CB A:ARG216 4.7 19.1 1.0 CB A:ASP179 4.7 21.6 1.0 N A:ARG216 4.8 23.0 1.0 C A:ASP215 4.8 24.2 1.0 CG A:ASP179 4.8 29.8 1.0 CA A:ARG216 4.8 19.6 1.0 O A:ASP215 4.8 25.8 1.0

M.Garcia-Alija, J.J.Du, I.Ordonez, A.Diz-Vallenilla, A.Moraleda-Montoya, N.Sultana, C.G.Huynh, C.Li, T.C.Donahue, L.X.Wang, B.Trastoy, E.J.Sundberg, M.E.Guerin. Mechanism of Cooperative N-Glycan Processing By the Multi-Modular Endoglycosidase Endoe. Nat Commun V. 13 1137 2022.
ISSN: ESSN 2041-1723
PubMed: 35241669
DOI: 10.1038/S41467-022-28722-W