Chlorine in PDB 8alh: X-Ray Structure of Human Ncs-1 Bound to Ric-8A

The structure of X-Ray Structure of Human Ncs-1 Bound to Ric-8A, PDB code: 8alh was solved by D.Munoz-Reyes, M.J.Sanchez-Barrena, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	52.38 / 1.86
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	56.861, 56.861, 134.612, 90, 90, 90
R / Rfree (%)	19.6 / 23.1

The structure of X-Ray Structure of Human Ncs-1 Bound to Ric-8A also contains other interesting chemical elements:

Calcium	(Ca)	2 atoms
Magnesium	(Mg)	1 atom
Sodium	(Na)	1 atom

The binding sites of Chlorine atom in the X-Ray Structure of Human Ncs-1 Bound to Ric-8A (pdb code 8alh). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the X-Ray Structure of Human Ncs-1 Bound to Ric-8A, PDB code: 8alh:

Chlorine binding site 1 out of 1 in the X-Ray Structure of Human Ncs-1 Bound to Ric-8A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of X-Ray Structure of Human Ncs-1 Bound to Ric-8A within 5.0Å range: probe atom residue distance (Å) B Occ P:Cl501 b:23.9 occ:1.00 H P:ALA416 2.3 32.8 1.0 HH B:TYR108 2.3 33.0 1.0 H P:GLY413 2.5 29.8 1.0 H P:GLY411 2.6 32.6 1.0 HB3 P:ALA415 2.8 32.1 1.0 H P:ALA415 3.0 34.1 1.0 OH B:TYR108 3.1 27.5 1.0 N P:ALA416 3.1 27.3 1.0 HA P:THR410 3.1 35.6 1.0 HA2 P:GLY413 3.2 28.1 1.0 HB2 P:ALA416 3.2 31.7 1.0 N P:GLY413 3.2 24.8 1.0 N P:GLY411 3.4 27.2 1.0 CA P:GLY413 3.4 23.4 1.0 C P:GLY413 3.5 24.1 1.0 HE1 B:TYR108 3.5 31.5 1.0 N P:ALA415 3.5 28.4 1.0 HB3 P:ALA416 3.5 31.7 1.0 HD11 B:ILE128 3.6 34.5 1.0 CB P:ALA415 3.6 26.7 1.0 O P:TYR409 3.7 28.1 1.0 CB P:ALA416 3.7 26.4 1.0 O P:GLY413 3.7 24.7 1.0 HD13 B:ILE128 3.8 34.5 1.0 H P:TYR412 3.8 31.7 1.0 CA P:ALA415 3.9 30.6 1.0 N P:ASN414 3.9 25.7 1.0 HD12 B:ILE128 3.9 34.5 1.0 C P:ALA415 3.9 27.5 1.0 CD1 B:ILE128 4.0 28.8 1.0 CA P:THR410 4.0 29.7 1.0 CA P:ALA416 4.0 26.8 1.0 H P:ASN414 4.0 30.8 1.0 CZ B:TYR108 4.0 29.6 1.0 HB2 P:ALA415 4.0 32.1 1.0 CE1 B:TYR108 4.1 26.2 1.0 N P:TYR412 4.1 26.4 1.0 C P:THR410 4.2 31.2 1.0 HG23 P:THR410 4.2 43.5 1.0 HA2 P:GLY411 4.2 30.6 1.0 CA P:GLY411 4.2 25.5 1.0 HB1 P:ALA415 4.3 32.1 1.0 HA3 P:GLY413 4.4 28.1 1.0 C P:ASN414 4.4 29.7 1.0 C P:GLY411 4.4 29.5 1.0 C P:TYR412 4.4 26.8 1.0 HA P:ALA416 4.5 32.2 1.0 C P:TYR409 4.5 28.5 1.0 HB1 P:ALA416 4.6 31.7 1.0 H P:GLY417 4.6 25.7 1.0 HG22 B:ILE124 4.7 32.1 1.0 N P:THR410 4.7 29.4 1.0 CA P:ASN414 4.8 28.7 1.0 HA P:ALA415 4.8 36.8 1.0 CA P:TYR412 4.9 28.0 1.0 HG21 B:ILE124 4.9 32.1 1.0 CG2 P:THR410 5.0 36.2 1.0 CB P:THR410 5.0 32.0 1.0

D.Munoz-Reyes, L.J.Mcclelland, S.Arroyo-Urea, S.Sanchez-Yepes, J.Sabin, S.Perez-Suarez, M.Menendez, A.Mansilla, J.Garcia-Nafria, S.Sprang, M.J.Sanchez-Barrena. The Neuronal Calcium Sensor Ncs-1 Regulates the Phosphorylation State and Activity of the Ga Chaperone and Gef Ric-8A. Elife V. 12 2023.
ISSN: ESSN 2050-084X
PubMed: 38018500
DOI: 10.7554/ELIFE.86151