Chlorine in PDB 8ft8: The Von Willebrand Factor A Domain of Human Capillary Morphogenesis Gene II, Flexibly Fused to the 1TEL Crystallization Chaperone, Thr- Val Linker Variant, Sumo Tag-Free Preparation

The structure of The Von Willebrand Factor A Domain of Human Capillary Morphogenesis Gene II, Flexibly Fused to the 1TEL Crystallization Chaperone, Thr- Val Linker Variant, Sumo Tag-Free Preparation, PDB code: 8ft8 was solved by P.L.Gajjar, C.M.Litchfield, M.Callahan, N.Redd, T.Doukov, J.D.Moody, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.87 / 1.60
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	100.882, 100.882, 49.754, 90, 90, 120
R / Rfree (%)	18.2 / 20.4

The structure of The Von Willebrand Factor A Domain of Human Capillary Morphogenesis Gene II, Flexibly Fused to the 1TEL Crystallization Chaperone, Thr- Val Linker Variant, Sumo Tag-Free Preparation also contains other interesting chemical elements:

Potassium

(K)

1 atom

The binding sites of Chlorine atom in the The Von Willebrand Factor A Domain of Human Capillary Morphogenesis Gene II, Flexibly Fused to the 1TEL Crystallization Chaperone, Thr- Val Linker Variant, Sumo Tag-Free Preparation (pdb code 8ft8). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the The Von Willebrand Factor A Domain of Human Capillary Morphogenesis Gene II, Flexibly Fused to the 1TEL Crystallization Chaperone, Thr- Val Linker Variant, Sumo Tag-Free Preparation, PDB code: 8ft8:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the The Von Willebrand Factor A Domain of Human Capillary Morphogenesis Gene II, Flexibly Fused to the 1TEL Crystallization Chaperone, Thr- Val Linker Variant, Sumo Tag-Free Preparation


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of The Von Willebrand Factor A Domain of Human Capillary Morphogenesis Gene II, Flexibly Fused to the 1TEL Crystallization Chaperone, Thr- Val Linker Variant, Sumo Tag-Free Preparation within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:46.6 occ:1.00 H A:HIS73 2.3 36.3 1.0 HG A:SER71 2.7 29.8 1.0 HD2 A:PRO72 2.7 36.1 1.0 HB2 A:HIS73 2.7 42.7 1.0 HD1 A:HIS73 2.9 77.4 1.0 O A:HOH479 2.9 40.1 1.0 HA A:SER71 3.1 27.5 1.0 N A:HIS73 3.1 30.2 1.0 ND1 A:HIS73 3.2 64.4 1.0 CB A:HIS73 3.4 35.5 1.0 OG A:SER71 3.4 24.8 1.0 CD A:PRO72 3.4 30.0 1.0 N A:PRO72 3.4 26.6 1.0 CG A:HIS73 3.5 43.2 1.0 C A:SER71 3.7 23.9 1.0 CA A:SER71 3.7 22.8 1.0 CA A:HIS73 3.8 32.4 1.0 H A:SER74 3.9 31.1 0.4 H A:SER74 3.9 31.1 0.6 HB2 A:PRO72 4.0 41.3 1.0 HD3 A:PRO72 4.0 36.1 1.0 O A:HOH417 4.1 36.3 1.0 C A:PRO72 4.1 31.1 1.0 CB A:SER71 4.1 24.3 1.0 CA A:PRO72 4.2 28.9 1.0 CE1 A:HIS73 4.2 63.9 1.0 HB3 A:HIS73 4.2 42.7 1.0 HG A:SER74 4.2 32.6 0.4 CG A:PRO72 4.4 39.4 1.0 CB A:PRO72 4.4 34.3 1.0 O A:SER71 4.4 24.7 1.0 HB2 A:SER71 4.4 29.2 1.0 N A:SER74 4.5 25.8 1.0 HG2 A:PRO72 4.5 47.4 1.0 CD2 A:HIS73 4.6 52.8 1.0 HA A:HIS73 4.6 39.0 1.0 HE1 A:HIS73 4.6 76.7 1.0 C A:HIS73 4.6 24.9 1.0 HG A:SER74 4.8 31.7 0.6 NE2 A:HIS73 4.9 58.2 1.0 HB3 A:SER71 4.9 29.2 1.0 O A:ARG70 5.0 26.1 1.0

Chlorine binding site 2 out of 3 in the The Von Willebrand Factor A Domain of Human Capillary Morphogenesis Gene II, Flexibly Fused to the 1TEL Crystallization Chaperone, Thr- Val Linker Variant, Sumo Tag-Free Preparation


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of The Von Willebrand Factor A Domain of Human Capillary Morphogenesis Gene II, Flexibly Fused to the 1TEL Crystallization Chaperone, Thr- Val Linker Variant, Sumo Tag-Free Preparation within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl303 b:48.9 occ:1.00 H A:ARG28 2.2 35.9 1.0 HB2 A:SER27 2.9 39.0 1.0 N A:ARG28 3.0 29.9 1.0 HA A:SER27 3.1 35.9 1.0 HB3 A:ASN55 3.1 30.6 1.0 HB2 A:ARG28 3.2 40.3 1.0 HB3 A:ARG28 3.5 40.3 1.0 O A:HOH497 3.5 36.1 1.0 CA A:SER27 3.7 29.8 1.0 CB A:SER27 3.7 32.5 1.0 CB A:ARG28 3.7 33.5 1.0 C A:SER27 3.8 29.7 1.0 O A:HOH524 3.9 57.0 1.0 HD22 A:ASN55 3.9 35.1 1.0 CA A:ARG28 4.0 26.9 1.0 CB A:ASN55 4.0 25.5 1.0 HB2 A:ASN55 4.1 30.6 1.0 HB3 A:SER27 4.1 39.0 1.0 H A:ASP29 4.4 33.6 1.0 HA A:ARG28 4.5 32.3 1.0 HA A:ASN55 4.6 32.2 1.0 ND2 A:ASN55 4.7 29.1 1.0 OG A:SER27 4.8 32.0 1.0 HD3 A:ARG28 4.8 52.5 1.0 CA A:ASN55 4.9 26.8 1.0 CG A:ASN55 4.9 26.9 1.0

Chlorine binding site 3 out of 3 in the The Von Willebrand Factor A Domain of Human Capillary Morphogenesis Gene II, Flexibly Fused to the 1TEL Crystallization Chaperone, Thr- Val Linker Variant, Sumo Tag-Free Preparation


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of The Von Willebrand Factor A Domain of Human Capillary Morphogenesis Gene II, Flexibly Fused to the 1TEL Crystallization Chaperone, Thr- Val Linker Variant, Sumo Tag-Free Preparation within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl304 b:83.9 occ:1.00 HZ2 A:LYS151 2.7 102.3 1.0 HG1 A:THR146 3.2 66.7 1.0 OG1 A:THR146 3.3 55.5 1.0 NZ A:LYS151 3.6 85.2 1.0 O A:HOH511 3.6 54.1 1.0 HB2 A:ASP148 3.6 83.6 1.0 HD2 A:LYS151 3.9 75.3 1.0 HZ1 A:LYS151 3.9 102.3 1.0 HZ3 A:LYS151 3.9 102.3 1.0 H A:ASP148 4.3 67.5 1.0 HB A:THR146 4.3 62.2 1.0 CB A:THR146 4.4 51.8 1.0 HE3 A:LYS151 4.4 88.8 1.0 CE A:LYS151 4.4 73.9 1.0 HB3 A:ASP148 4.4 83.6 1.0 CB A:ASP148 4.4 69.6 1.0 H A:GLY147 4.5 57.7 1.0 HA A:THR146 4.5 57.1 1.0 CD A:LYS151 4.6 62.7 1.0 HD3 A:LYS151 4.9 75.3 1.0 N A:ASP148 5.0 56.2 1.0 CA A:THR146 5.0 47.5 1.0

P.L.Gajjar, M.J.Pedroza Romo, C.M.Litchfield, M.Callahan, N.Redd, S.Nawarathange, E.Wilson, J.Averett, S.Soleimani, C.Stewart, A.Lewis, T.Smith, S.Brown, Y.J.Tseng, T.Doukov, A.Lebedev, M.D.Moody. Decreasing the Flexibility of the Telsam-Target Protein Linker and Omitting the Cleavable Fusion Tag Improves Crystal Order and Diffraction Limits To Be Published.