Chlorine in PDB 8pi7: Dna Binding Domain of Hnf-1A Bound to P2-HNF4A Promoter Dna Variant (P2 -169C>T)

The structure of Dna Binding Domain of Hnf-1A Bound to P2-HNF4A Promoter Dna Variant (P2 -169C>T), PDB code: 8pi7 was solved by L.Kind, M.Myllykoski, A.Raasakka, P.Kursula, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.14 / 3.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	49.456, 52.506, 210.278, 90, 90, 90
R / Rfree (%)	22.1 / 28

The binding sites of Chlorine atom in the Dna Binding Domain of Hnf-1A Bound to P2-HNF4A Promoter Dna Variant (P2 -169C>T) (pdb code 8pi7). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Dna Binding Domain of Hnf-1A Bound to P2-HNF4A Promoter Dna Variant (P2 -169C>T), PDB code: 8pi7:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Dna Binding Domain of Hnf-1A Bound to P2-HNF4A Promoter Dna Variant (P2 -169C>T)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Dna Binding Domain of Hnf-1A Bound to P2-HNF4A Promoter Dna Variant (P2 -169C>T) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl301 b:127.7 occ:1.00 NH1 A:ARG244 3.2 120.1 1.0 O A:HIS126 3.6 129.5 1.0 O A:GLY253 4.0 141.7 1.0 CB A:HIS126 4.0 95.4 1.0 O A:LEU254 4.1 141.5 1.0 CD2 A:HIS126 4.2 112.0 1.0 ND2 A:ASN257 4.2 146.2 1.0 CA A:LEU254 4.3 112.6 1.0 NE2 A:GLN176 4.4 197.2 1.0 CZ A:PHE177 4.5 147.1 1.0 CE1 A:PHE177 4.5 160.2 1.0 CG A:HIS126 4.5 121.0 1.0 CZ A:ARG244 4.5 113.4 1.0 C A:HIS126 4.5 130.2 1.0 CA A:HIS126 4.5 117.3 1.0 C A:LEU254 4.6 125.4 1.0 CD2 A:LEU254 4.6 102.8 1.0 C A:GLY253 4.8 133.8 1.0 OE1 A:GLN176 4.9 195.3 1.0 CD A:GLN176 4.9 194.8 1.0

Chlorine binding site 2 out of 2 in the Dna Binding Domain of Hnf-1A Bound to P2-HNF4A Promoter Dna Variant (P2 -169C>T)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Dna Binding Domain of Hnf-1A Bound to P2-HNF4A Promoter Dna Variant (P2 -169C>T) within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl301 b:114.6 occ:1.00 O B:LEU254 3.6 128.6 1.0 NE B:ARG244 3.6 116.2 1.0 O B:HIS126 3.7 113.5 1.0 CE1 B:PHE177 4.0 146.9 1.0 CB B:HIS126 4.0 101.8 1.0 NE2 B:GLN176 4.1 142.6 1.0 CZ B:PHE177 4.1 131.8 1.0 ND2 B:ASN257 4.3 140.0 1.0 CD B:ARG244 4.3 94.4 1.0 CD2 B:HIS126 4.3 113.6 1.0 C B:LEU254 4.3 108.7 1.0 NH2 B:ARG244 4.5 96.8 1.0 O B:GLY253 4.5 140.9 1.0 CZ B:ARG244 4.5 120.7 1.0 CA B:LEU254 4.5 118.0 1.0 CA B:HIS126 4.5 113.9 1.0 C B:HIS126 4.5 106.2 1.0 CG B:HIS126 4.6 128.2 1.0 CG1 B:ILE128 4.9 106.7 1.0 CG B:ASN257 4.9 116.1 1.0

L.Kind, J.Molnes, E.Tjora, A.Raasakka, M.Myllykoski, K.Colclough, C.Saint-Martin, C.Adelfalk, P.Dusatkova, S.Pruhova, C.Valtonen-Andre, C.Bellanne-Chantelot, T.Arnesen, P.Kursula, P.R.Njolstad. Molecular Mechanism of Hnf-1A-Mediated HNF4A Gene Regulation and Promoter-Driven HNF4A-Mody Diabetes. Jci Insight V. 9 2024.
ISSN: ISSN 2379-3708
PubMed: 38855865
DOI: 10.1172/JCI.INSIGHT.175278