Chlorine in PDB 8x5v: BLCAS9-Sgrna-Target Dna Complex

The structure of BLCAS9-Sgrna-Target Dna Complex, PDB code: 8x5v was solved by T.Nakane, R.Nakagawa, K.Yamashita, H.Nishimasu, O.Nureki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	118.39 / 2.00
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	144.75, 99.21, 119.3, 90, 97.09, 90
R / Rfree (%)	19.8 / 23.1

The binding sites of Chlorine atom in the BLCAS9-Sgrna-Target Dna Complex (pdb code 8x5v). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the BLCAS9-Sgrna-Target Dna Complex, PDB code: 8x5v:

Chlorine binding site 1 out of 1 in the BLCAS9-Sgrna-Target Dna Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of BLCAS9-Sgrna-Target Dna Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1127 b:68.3 occ:1.00 N A:LYS179 3.1 46.2 1.0 NE2 A:HIS175 3.5 61.9 1.0 CA A:ASN178 3.5 38.5 1.0 O A:LYS179 3.6 53.7 1.0 CB A:HIS184 3.6 60.2 1.0 OD1 A:ASN178 3.6 42.6 1.0 O A:HOH1308 3.6 52.1 1.0 CB A:ASN178 3.7 42.1 1.0 CG A:HIS184 3.7 69.1 1.0 CB A:ASN182 3.8 53.1 1.0 N A:HIS184 3.8 45.4 1.0 C A:ASN178 3.8 39.3 1.0 ND1 A:HIS184 3.8 74.1 1.0 C A:ASN182 4.0 48.4 1.0 CD2 A:HIS175 4.1 53.1 1.0 CG A:ASN178 4.1 45.7 1.0 O A:ASN182 4.1 41.5 1.0 CA A:LYS179 4.2 49.6 1.0 CA A:HIS184 4.3 49.6 1.0 C A:LYS179 4.3 56.6 1.0 CA A:ASN182 4.4 51.2 1.0 N A:TYR183 4.4 41.4 1.0 CD2 A:HIS184 4.5 61.9 1.0 CE1 A:HIS175 4.5 61.9 1.0 CE1 A:HIS184 4.6 72.3 1.0 CB A:LYS179 4.6 48.8 1.0 N A:ASN178 4.7 37.6 1.0 O A:ARG177 4.7 43.5 1.0 N A:ASN182 4.8 48.0 1.0 C A:TYR183 4.8 44.9 1.0 CG A:ASN182 4.9 71.2 1.0 NE2 A:HIS184 4.9 72.7 1.0 N A:GLY185 4.9 40.4 1.0 OD1 A:ASN182 5.0 73.4 1.0 CA A:TYR183 5.0 44.3 1.0

T.Nakane, R.Nakagawa, S.Ishiguro, S.Okazaki, H.Mori, Y.Shuto, K.Yamashita, N.Yachie, H.Nishimasu, O.Nureki. Structure and Engineering of Brevibacillus Laterosporus CAS9. Commun Biol V. 7 803 2024.
ISSN: ESSN 2399-3642
PubMed: 38961195
DOI: 10.1038/S42003-024-06422-Z