Chlorine in PDB 9ci7: Structure of Pnuts:TOX4 Complex

The structure of Structure of Pnuts:TOX4 Complex, PDB code: 9ci7 was solved by R.Page, W.Peti, X.Wang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.32 / 2.10
Space group	P 61
Cell size a, b, c (Å), α, β, γ (°)	70.63, 70.63, 97.165, 90, 90, 120
R / Rfree (%)	18.8 / 21.7

The structure of Structure of Pnuts:TOX4 Complex also contains other interesting chemical elements:

Zinc	(Zn)	2 atoms
Sodium	(Na)	1 atom

The binding sites of Chlorine atom in the Structure of Pnuts:TOX4 Complex (pdb code 9ci7). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Structure of Pnuts:TOX4 Complex, PDB code: 9ci7:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Structure of Pnuts:TOX4 Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of Pnuts:TOX4 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl201 b:47.1 occ:1.00 ZN A:ZN204 2.1 42.0 1.0 NE2 B:HIS569 3.4 42.5 1.0 NE2 A:HIS3 3.4 34.4 1.0 CD2 A:HIS3 3.8 43.1 1.0 CD A:PRO5 3.8 42.5 1.0 CG A:PRO5 4.1 41.3 1.0 CE1 B:HIS569 4.1 40.6 1.0 CD2 B:HIS569 4.4 38.3 1.0 CE1 A:HIS3 4.5 42.9 1.0 O A:HIS3 4.9 59.2 1.0 CG A:HIS3 4.9 45.5 1.0

Chlorine binding site 2 out of 4 in the Structure of Pnuts:TOX4 Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of Pnuts:TOX4 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl202 b:50.4 occ:1.00 N A:ARG42 3.3 38.7 1.0 N A:LYS43 3.3 37.0 1.0 OG A:SER46 3.4 43.5 1.0 CB A:LYS43 3.7 43.6 1.0 O A:HOH336 3.8 49.1 1.0 CA A:ALA41 3.8 39.0 1.0 CG A:LYS43 3.8 44.6 1.0 CB A:SER46 3.8 32.4 1.0 C A:ALA41 4.0 40.8 1.0 CA A:LYS43 4.1 42.6 1.0 CD1 A:ILE6 4.2 44.1 1.0 CA A:ARG42 4.3 44.7 1.0 C A:ARG42 4.3 44.4 1.0 CB A:ALA41 4.3 30.4 1.0 O A:GLU40 4.4 48.1 1.0 O A:LYS43 4.7 36.2 1.0 CG1 A:ILE6 4.8 37.9 1.0 CB A:ARG42 4.8 43.5 1.0 C A:LYS43 4.9 40.9 1.0 N A:ALA41 4.9 36.8 1.0

Chlorine binding site 3 out of 4 in the Structure of Pnuts:TOX4 Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Structure of Pnuts:TOX4 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl203 b:74.3 occ:1.00 O A:HOH343 3.1 64.3 1.0 NH2 A:ARG47 3.3 42.2 1.0 CB A:TYR81 3.9 34.6 1.0 O A:HOH335 3.9 37.0 1.0 NH1 A:ARG47 4.0 35.3 1.0 CG A:TYR81 4.0 41.6 1.0 CZ A:ARG47 4.1 42.8 1.0 OG A:SER77 4.2 60.1 1.0 CD1 A:TYR81 4.3 36.1 1.0 O A:SER77 4.5 33.3 1.0 NH1 A:ARG42 4.6 88.7 1.0 CD2 A:TYR81 4.6 38.7 1.0 CD A:ARG42 4.8 81.3 1.0 CZ A:ARG42 5.0 103.5 1.0

Chlorine binding site 4 out of 4 in the Structure of Pnuts:TOX4 Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Structure of Pnuts:TOX4 Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl702 b:56.7 occ:1.00 N B:GLU595 3.3 35.9 1.0 N B:ASN594 3.5 35.9 1.0 CG B:GLU595 3.7 60.8 1.0 CB B:ASN594 3.8 38.6 1.0 O A:HOH359 3.8 43.5 1.0 CB B:SER593 3.8 32.2 1.0 CB B:GLU595 3.9 36.9 1.0 O B:HOH837 4.0 42.1 1.0 CA B:ASN594 4.0 31.0 1.0 C B:ASN594 4.1 34.9 1.0 CG A:PRO89 4.1 35.3 1.0 CA B:GLU595 4.1 34.2 1.0 OG B:SER593 4.3 35.0 1.0 C B:SER593 4.4 45.3 1.0 CD A:PRO89 4.5 34.4 1.0 CD B:GLU595 4.5 93.6 1.0 CA B:SER593 4.6 32.2 1.0 ND2 B:ASN594 4.6 35.5 1.0 CG B:ASN594 4.6 31.1 1.0

L.Duncalf, X.Wang, A.A.Aljabri, A.E.Campbell, R.Q.Alharbi, I.Donaldson, A.Hayes, W.Peti, R.Page, D.Bennett. Pnuts:PP1 Recruitment to TOX4 Regulates Chromosomal Dispersal in Drosophila Germline Development. Cell Rep V. 44 15693 2025.
ISSN: ESSN 2211-1247
PubMed: 40347473
DOI: 10.1016/J.CELREP.2025.115693