Chlorine in PDB 9fc7: Hen Egg-White Lysozyme Incubated at 99% Relative Humidity

All present enzymatic activity of Hen Egg-White Lysozyme Incubated at 99% Relative Humidity:
3.2.1.17;

The structure of Hen Egg-White Lysozyme Incubated at 99% Relative Humidity, PDB code: 9fc7 was solved by P.Y.A.Reinke, S.Guenther, S.Falke, M.Galchenkova, A.Rahmani Mashhour, A.Creon, J.Lieske, W.Ewert, A.C.Rorigues, S.Thekku Veedu, P.Fischer, A.Meents, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.63 / 1.40
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	79.662, 79.662, 37.709, 90, 90, 90
R / Rfree (%)	15.3 / 18.6

The structure of Hen Egg-White Lysozyme Incubated at 99% Relative Humidity also contains other interesting chemical elements:

Sodium

(Na)

2 atoms

The binding sites of Chlorine atom in the Hen Egg-White Lysozyme Incubated at 99% Relative Humidity (pdb code 9fc7). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Hen Egg-White Lysozyme Incubated at 99% Relative Humidity, PDB code: 9fc7:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Hen Egg-White Lysozyme Incubated at 99% Relative Humidity


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Hen Egg-White Lysozyme Incubated at 99% Relative Humidity within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl207 b:24.5 occ:1.00 HH A:TYR23 2.3 22.2 1.0 OH A:TYR23 3.0 18.5 1.0 HE2 A:TYR23 3.1 19.8 1.0 H12 A:EDO201 3.3 54.3 1.0 O A:HOH352 3.3 55.5 1.0 H11 A:EDO201 3.4 54.3 1.0 O A:HOH336 3.4 28.5 1.0 HA3 A:GLY104 3.4 22.2 1.0 CE2 A:TYR23 3.7 16.5 1.0 CZ A:TYR23 3.7 17.3 1.0 C1 A:EDO201 3.8 45.2 1.0 HA2 A:GLY104 4.1 22.2 1.0 CA A:GLY104 4.1 18.5 1.0 H21 A:EDO201 4.4 66.0 1.0 O A:ARG21 4.6 24.4 0.5 O A:ARG21 4.6 24.3 0.5 N A:GLY104 4.7 18.8 1.0 C2 A:EDO201 4.8 55.0 1.0 H A:MET105 4.8 19.9 1.0 H A:GLY104 4.9 22.5 1.0 HB3 A:ARG21 4.9 32.2 0.5 CE1 A:TYR23 4.9 16.8 1.0 O1 A:EDO201 4.9 26.9 1.0 CD2 A:TYR23 4.9 17.0 1.0 HB3 A:ARG21 5.0 31.9 0.5

Chlorine binding site 2 out of 3 in the Hen Egg-White Lysozyme Incubated at 99% Relative Humidity


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Hen Egg-White Lysozyme Incubated at 99% Relative Humidity within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl208 b:36.9 occ:1.00 H A:SER24 2.2 24.0 1.0 HG A:SER24 2.6 32.7 1.0 HD21 A:ASN27 2.8 24.1 1.0 O A:HOH337 2.9 43.6 0.6 HB3 A:SER24 3.0 26.6 1.0 N A:SER24 3.1 20.0 1.0 HA A:TYR23 3.2 23.0 1.0 HD22 A:ASN27 3.3 24.1 1.0 OG A:SER24 3.3 27.2 1.0 O A:GLY22 3.3 23.1 1.0 ND2 A:ASN27 3.3 20.0 1.0 CB A:SER24 3.5 22.2 1.0 O A:HOH337 3.7 36.8 0.4 CA A:SER24 3.9 19.8 1.0 CA A:TYR23 4.0 19.2 1.0 C A:TYR23 4.0 18.2 1.0 O A:HOH329 4.2 45.8 1.0 C A:GLY22 4.3 20.8 1.0 HB2 A:SER24 4.4 26.6 1.0 HA A:SER24 4.5 23.8 1.0 CG A:ASN27 4.6 18.2 1.0 N A:TYR23 4.6 20.2 1.0 OD1 A:ASN19 4.8 29.4 0.4 OD1 A:ASN19 4.8 44.3 0.6 HD1 A:TYR23 4.9 20.7 1.0 HG21 A:VAL120 5.0 31.8 1.0 C A:SER24 5.0 18.4 1.0

Chlorine binding site 3 out of 3 in the Hen Egg-White Lysozyme Incubated at 99% Relative Humidity


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Hen Egg-White Lysozyme Incubated at 99% Relative Humidity within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl209 b:66.8 occ:1.00 HH22 A:ARG14 2.4 34.2 0.5 H A:ILE88 2.6 26.8 1.0 HG12 A:ILE88 2.7 30.5 1.0 HA A:ASP87 2.9 28.6 1.0 NH2 A:ARG14 3.2 28.5 0.5 N A:ILE88 3.4 22.3 1.0 HH21 A:ARG14 3.4 34.2 0.5 CG1 A:ILE88 3.7 25.4 1.0 OD1 A:ASP87 3.7 33.0 1.0 HB1 A:ALA11 3.8 29.9 1.0 HG23 A:ILE88 3.8 31.1 1.0 CA A:ASP87 3.8 23.8 1.0 HG13 A:ILE88 3.9 30.5 1.0 HZ A:PHE3 3.9 33.3 1.0 HH12 A:ARG14 3.9 55.5 0.5 NE2 A:HIS15 4.0 38.7 1.0 C A:ASP87 4.1 26.5 1.0 O A:SER86 4.2 29.6 1.0 CZ A:ARG14 4.2 41.8 0.5 H A:THR89 4.4 27.2 1.0 CA A:ILE88 4.4 21.2 1.0 CB A:ILE88 4.4 20.2 1.0 HB2 A:ALA11 4.4 29.9 1.0 CG A:ASP87 4.4 35.7 1.0 NH1 A:ARG14 4.5 46.2 0.5 HD11 A:ILE88 4.5 32.1 1.0 HD2 A:HIS15 4.5 35.6 1.0 CB A:ALA11 4.5 24.9 1.0 CG2 A:ILE88 4.6 25.9 1.0 CD2 A:HIS15 4.6 29.7 1.0 CD1 A:ILE88 4.6 26.8 1.0 CB A:ASP87 4.6 29.3 1.0 CZ A:PHE3 4.8 27.8 1.0 HD13 A:ILE88 4.8 32.1 1.0 N A:ASP87 4.8 23.1 1.0 N A:THR89 4.9 22.7 1.0 HB2 A:ASP87 4.9 35.2 1.0 HE2 A:PHE3 4.9 30.6 1.0 C A:SER86 4.9 24.6 1.0 CE1 A:HIS15 5.0 37.6 1.0

P.Y.A.Reinke, A.Meents. Exploring the Conformational Space of Proteins By Systematic Variation of the Relative Humidity To Be Published.