Chlorine in PDB 9ggj: Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate

Enzymatic activity of Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate

All present enzymatic activity of Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate:
4.3.2.1;

Protein crystallography data

The structure of Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate, PDB code: 9ggj was solved by M.Nielipinski, A.J.Pietrzyk-Brzezinska, D.Krzeszewska, B.Sekula, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 49.57 / 2.00
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 106.588, 230.848, 112.534, 90, 89.48, 90
R / Rfree (%) 17.5 / 22.7

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate (pdb code 9ggj). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 7 binding sites of Chlorine where determined in the Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate, PDB code: 9ggj:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5; 6; 7;

Chlorine binding site 1 out of 7 in 9ggj

Go back to Chlorine Binding Sites List in 9ggj
Chlorine binding site 1 out of 7 in the Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl607

b:34.8
occ:1.00
 O B:HOH871 3.2 27.1 1.0
N A:TYR493 3.3 20.4 1.0
N B:ARG265 3.4 23.1 1.0
O B:ILE263 3.5 25.5 1.0
CG B:ARG265 3.7 21.7 1.0
CB A:TYR493 3.7 20.4 1.0
CB B:ARG265 3.8 22.1 1.0
CA A:SER492 4.0 21.3 1.0
CA B:ASP264 4.0 24.8 1.0
CA A:TYR493 4.1 20.1 1.0
C A:SER492 4.1 21.2 1.0
CA B:ARG265 4.2 22.3 1.0
CB A:SER492 4.2 20.6 1.0
C B:ASP264 4.2 25.0 1.0
C B:ILE263 4.2 24.9 1.0
CD B:ARG265 4.3 21.5 1.0
N B:ASP264 4.5 25.3 1.0
CG1 B:ILE263 4.6 23.6 1.0
CD1 B:PHE266 4.9 23.0 1.0

Chlorine binding site 2 out of 7 in 9ggj

Go back to Chlorine Binding Sites List in 9ggj
Chlorine binding site 2 out of 7 in the Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl608

b:31.7
occ:1.00
 O A:HOH732 2.9 35.0 1.0
N B:TYR493 3.3 21.0 1.0
N A:ARG265 3.4 23.3 1.0
O A:ILE263 3.5 26.4 1.0
CG A:ARG265 3.7 20.4 1.0
CB B:TYR493 3.7 20.8 1.0
CB A:ARG265 3.9 20.9 1.0
CA B:SER492 3.9 22.5 1.0
O A:HOH951 4.0 46.4 1.0
CA A:ASP264 4.0 25.3 1.0
C B:SER492 4.1 22.0 1.0
CA B:TYR493 4.1 20.9 1.0
C A:ILE263 4.1 24.9 1.0
CB B:SER492 4.2 21.7 1.0
CG1 A:ILE263 4.2 23.9 1.0
C A:ASP264 4.2 24.3 1.0
CA A:ARG265 4.3 22.1 1.0
CD A:ARG265 4.3 19.9 1.0
N A:ASP264 4.4 25.7 1.0
O A:HOH728 4.8 32.2 1.0
CD1 A:ILE263 4.9 24.5 1.0

Chlorine binding site 3 out of 7 in 9ggj

Go back to Chlorine Binding Sites List in 9ggj
Chlorine binding site 3 out of 7 in the Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl704

b:23.0
occ:1.00
 N D:ARG265 3.2 18.9 1.0
N C:TYR493 3.3 19.1 1.0
NE2 H:HIS103 3.4 25.3 1.0
CE1 H:HIS103 3.5 25.1 1.0
O D:ILE263 3.5 22.6 1.0
CB C:TYR493 3.6 19.6 1.0
CG D:ARG265 3.7 19.1 1.0
CA D:ASP264 3.9 22.2 1.0
CB D:ARG265 3.9 18.0 1.0
CA C:SER492 4.0 19.8 1.0
C D:ASP264 4.0 19.7 1.0
CA C:TYR493 4.1 19.0 1.0
C D:ILE263 4.2 22.6 1.0
C C:SER492 4.2 19.0 1.0
CA D:ARG265 4.2 18.2 1.0
CB C:SER492 4.3 18.6 1.0
N D:ASP264 4.3 21.9 1.0
CD D:ARG265 4.4 18.9 1.0
CD2 H:HIS103 4.6 24.1 1.0
CG1 D:ILE263 4.7 24.5 1.0
ND1 H:HIS103 4.7 23.6 1.0
CD2 D:PHE266 4.8 21.2 1.0
CE H:MET267 4.8 23.5 0.5
OD1 D:ASP264 4.8 26.1 1.0
N D:PHE266 4.9 17.1 1.0
CG C:TYR493 5.0 19.4 1.0

Chlorine binding site 4 out of 7 in 9ggj

Go back to Chlorine Binding Sites List in 9ggj
Chlorine binding site 4 out of 7 in the Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl705

b:26.9
occ:1.00
 N D:TYR493 3.2 17.1 1.0
O G:HOH778 3.3 23.7 1.0
N C:ARG265 3.3 21.6 1.0
CG C:ARG265 3.6 20.4 1.0
CB D:TYR493 3.8 17.1 1.0
O3 G:GOL603 3.8 57.9 1.0
CA C:ASP264 3.8 24.4 1.0
CB C:ARG265 3.9 20.2 1.0
CA D:SER492 4.0 17.8 1.0
O C:ILE263 4.0 23.7 1.0
C C:ASP264 4.1 23.0 1.0
C D:SER492 4.1 17.6 1.0
CA D:TYR493 4.1 16.9 1.0
CB D:SER492 4.2 17.5 1.0
CA C:ARG265 4.2 21.8 1.0
CG1 C:ILE263 4.3 24.1 1.0
CD C:ARG265 4.3 19.9 1.0
C C:ILE263 4.3 23.5 1.0
C2 G:GOL603 4.4 55.0 1.0
N C:ASP264 4.4 24.7 1.0
C3 G:GOL603 4.6 56.8 1.0
CD1 C:ILE263 4.7 24.7 1.0
O1 G:GOL603 4.7 52.3 1.0
CG C:ASP264 4.8 28.4 1.0
CB C:ASP264 4.8 26.6 1.0
OD2 C:ASP264 4.9 30.4 1.0

Chlorine binding site 5 out of 7 in 9ggj

Go back to Chlorine Binding Sites List in 9ggj
Chlorine binding site 5 out of 7 in the Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl706

b:22.4
occ:1.00
 N H:TYR493 3.2 18.7 1.0
O C:HOH1126 3.2 24.7 1.0
NE2 C:HIS103 3.3 24.3 1.0
N G:ARG265 3.3 20.2 1.0
CE1 C:HIS103 3.3 23.7 1.0
O G:ILE263 3.6 23.9 1.0
CB H:TYR493 3.7 19.4 1.0
CG G:ARG265 3.7 20.4 1.0
CA G:ASP264 3.9 22.7 1.0
CB G:ARG265 3.9 19.3 1.0
CA H:SER492 3.9 18.6 1.0
CA H:TYR493 4.1 18.9 1.0
C H:SER492 4.1 18.4 1.0
C G:ASP264 4.1 21.6 1.0
CA G:ARG265 4.2 19.5 1.0
C G:ILE263 4.2 22.3 1.0
CB H:SER492 4.3 18.6 1.0
N G:ASP264 4.4 22.3 1.0
CD G:ARG265 4.5 19.9 1.0
CD2 C:HIS103 4.5 23.9 1.0
ND1 C:HIS103 4.5 24.4 1.0
CG1 G:ILE263 4.6 21.7 1.0
OD1 G:ASP264 4.8 24.3 1.0
CD2 G:PHE266 4.8 17.0 1.0
O H:HOH808 4.9 25.6 1.0
O H:SER491 4.9 18.5 1.0
N G:PHE266 5.0 18.2 1.0

Chlorine binding site 6 out of 7 in 9ggj

Go back to Chlorine Binding Sites List in 9ggj
Chlorine binding site 6 out of 7 in the Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 6 of Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Cl607

b:32.6
occ:1.00
 O E:HOH884 3.1 29.9 1.0
N F:TYR493 3.3 23.8 1.0
N E:ARG265 3.3 24.5 1.0
O E:ILE263 3.5 26.1 1.0
CG E:ARG265 3.7 24.1 1.0
CB F:TYR493 3.7 23.7 1.0
CB E:ARG265 3.8 23.3 1.0
CA E:ASP264 4.0 25.8 1.0
CA F:SER492 4.0 22.7 1.0
C E:ILE263 4.1 24.8 1.0
CA F:TYR493 4.2 23.4 1.0
C E:ASP264 4.2 24.8 1.0
CA E:ARG265 4.2 23.8 1.0
C F:SER492 4.2 23.3 1.0
CB F:SER492 4.2 23.0 1.0
CD E:ARG265 4.3 24.5 1.0
O E:HOH916 4.3 49.1 1.0
N E:ASP264 4.4 26.0 1.0
CG1 E:ILE263 4.4 21.8 1.0
CD1 E:ILE263 4.9 21.2 1.0

Chlorine binding site 7 out of 7 in 9ggj

Go back to Chlorine Binding Sites List in 9ggj
Chlorine binding site 7 out of 7 in the Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 7 of Crystal Structure of Argininosuccinate Lyase From Arabidopsis Thaliana (Atasl) in Complex with Biological Substrate and Products - Argininosuccinate, Argnine and Fumarate within 5.0Å range:
probe atom residue distance (Å) B Occ
G:Cl605

b:23.2
occ:1.00
 N G:TYR493 3.1 17.4 1.0
O H:HOH737 3.2 37.0 1.0
O H:HOH932 3.3 24.6 1.0
N H:ARG265 3.5 18.2 1.0
CB G:TYR493 3.6 18.9 1.0
CG H:ARG265 3.7 19.7 1.0
CA G:SER492 3.8 18.3 1.0
O H:ILE263 3.8 23.6 1.0
O G:HOH986 3.9 43.0 1.0
C G:SER492 3.9 17.8 1.0
CA H:ASP264 4.0 20.8 1.0
CA G:TYR493 4.0 18.1 1.0
CB H:ARG265 4.0 19.4 1.0
CB G:SER492 4.1 18.2 1.0
C H:ASP264 4.3 19.2 1.0
CD H:ARG265 4.3 19.2 1.0
CA H:ARG265 4.3 19.7 1.0
C H:ILE263 4.4 24.2 1.0
CG1 H:ILE263 4.5 26.9 1.0
N H:ASP264 4.5 22.8 1.0
O G:HOH1035 4.9 39.5 1.0
CG H:ASP264 4.9 22.5 1.0
OD2 H:ASP264 4.9 22.9 1.0
O H:HOH849 4.9 29.5 1.0
CB H:ASP264 5.0 21.0 1.0
O G:TYR493 5.0 17.5 1.0

Reference:

M.Nielipinski, D.Krzeszewska, A.J.Pietrzyk-Brzezinska, B.Sekula. Arabidopsis Thaliana Argininosuccinate Lyase Structure Uncovers the Role of Serine As the Catalytic Base. J.Struct.Biol. 08130 2024.
ISSN: ESSN 1095-8657
PubMed: 39384000
DOI: 10.1016/J.JSB.2024.108130
Page generated: Thu Oct 31 18:12:26 2024

Last articles

Zn in 9IRQ
Zn in 9IYX
Zn in 9J8P
Zn in 9IUU
Zn in 9GBF
Zn in 9G2V
Zn in 9G2L
Zn in 9G2X
Zn in 9G2Z
Zn in 9G2K
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy