Chlorine in PDB 9ihs: Microbial Transglutaminase Mutant - D3C/G283C

All present enzymatic activity of Microbial Transglutaminase Mutant - D3C/G283C:
2.3.2.13;

The structure of Microbial Transglutaminase Mutant - D3C/G283C, PDB code: 9ihs was solved by M.Suzuki, M.Date, T.Kashiwagi, K.Takahashi, A.Nakamura, M.Tanokura, E.Suzuki, K.Yokoyama, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.08 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	78.76, 117.36, 85.47, 90, 113.03, 90
R / Rfree (%)	18.1 / 23.5

The binding sites of Chlorine atom in the Microbial Transglutaminase Mutant - D3C/G283C (pdb code 9ihs). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Microbial Transglutaminase Mutant - D3C/G283C, PDB code: 9ihs:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Microbial Transglutaminase Mutant - D3C/G283C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Microbial Transglutaminase Mutant - D3C/G283C within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl402 b:39.8 occ:1.00 NE2 A:HIS279 2.9 24.6 1.0 CD A:PRO9 3.4 30.0 1.0 ND2 A:ASN32 3.7 24.1 1.0 CA A:PRO8 3.7 25.5 1.0 CD2 A:HIS279 3.8 22.6 1.0 CE1 A:HIS279 3.9 24.8 1.0 CB A:PRO8 4.1 28.0 1.0 CG1 A:VAL6 4.3 28.1 1.0 CG A:PRO281 4.3 24.5 1.0 N A:PRO9 4.5 27.1 1.0 N A:PRO8 4.5 27.4 1.0 CG A:PRO9 4.6 30.9 1.0 CG A:ASN32 4.6 22.8 1.0 C A:PRO8 4.7 28.1 1.0 OD1 A:ASN32 4.7 24.1 1.0 O A:THR7 4.8 24.1 1.0 CB A:PRO281 4.8 25.5 1.0 CG A:HIS279 5.0 24.6 1.0

Chlorine binding site 2 out of 4 in the Microbial Transglutaminase Mutant - D3C/G283C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Microbial Transglutaminase Mutant - D3C/G283C within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl403 b:32.9 occ:1.00 O B:HOH781 2.9 50.8 1.0 NE2 B:HIS279 3.0 28.0 1.0 ND2 B:ASN32 3.5 28.0 1.0 CD B:PRO9 3.6 27.7 1.0 CD2 B:HIS279 3.9 24.5 1.0 O B:HOH673 3.9 28.1 1.0 CE1 B:HIS279 3.9 28.7 1.0 CA B:PRO8 4.0 25.7 1.0 CG1 B:VAL6 4.3 29.3 1.0 OD1 B:ASN32 4.4 27.0 1.0 CG B:ASN32 4.4 26.7 1.0 CG B:PRO281 4.5 28.1 1.0 CB B:PRO8 4.6 25.9 1.0 O B:HOH599 4.6 42.0 1.0 CG B:PRO9 4.7 31.4 1.0 N B:PRO9 4.7 28.9 1.0 N B:PRO8 4.8 27.1 1.0 C B:PRO8 4.9 28.9 1.0 O B:THR7 4.9 28.6 1.0

Chlorine binding site 3 out of 4 in the Microbial Transglutaminase Mutant - D3C/G283C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Microbial Transglutaminase Mutant - D3C/G283C within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl402 b:21.5 occ:1.00 NE2 C:HIS279 3.1 14.9 1.0 O C:HOH797 3.2 44.3 1.0 ND2 C:ASN32 3.3 21.6 1.0 CD C:PRO9 3.7 23.7 1.0 CD2 C:HIS279 4.0 17.2 1.0 CE1 C:HIS279 4.0 16.7 1.0 CA C:PRO8 4.1 18.3 1.0 CG C:ASN32 4.2 24.9 1.0 CG1 C:VAL6 4.3 18.7 1.0 CG B:PRO19 4.3 20.9 1.0 CG C:PRO281 4.4 18.9 1.0 OD1 C:ASN32 4.4 24.9 1.0 CD B:PRO19 4.6 19.2 1.0 CB C:PRO8 4.7 18.5 1.0 CG C:PRO9 4.7 25.1 1.0 N C:PRO9 4.8 23.7 1.0 N C:PRO8 4.9 17.7 1.0 CB C:PRO281 4.9 19.1 1.0 O C:THR7 4.9 18.2 1.0

Chlorine binding site 4 out of 4 in the Microbial Transglutaminase Mutant - D3C/G283C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Microbial Transglutaminase Mutant - D3C/G283C within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl402 b:27.4 occ:1.00 O D:HOH673 2.8 36.5 1.0 NE2 D:HIS279 3.1 19.8 1.0 ND2 D:ASN32 3.4 23.3 1.0 CD D:PRO9 3.8 27.6 1.0 CD2 D:HIS279 4.0 19.0 1.0 CE1 D:HIS279 4.1 20.2 1.0 CA D:PRO8 4.2 23.7 1.0 CG A:PRO19 4.3 23.8 1.0 CG D:ASN32 4.3 27.3 1.0 CG1 D:VAL6 4.4 20.9 1.0 CG D:PRO281 4.4 23.9 1.0 OD1 D:ASN32 4.4 28.5 1.0 O A:HOH688 4.5 34.1 1.0 CD A:PRO19 4.5 22.3 1.0 CB D:PRO8 4.8 23.6 1.0 N D:PRO9 4.9 26.4 1.0 CG D:PRO9 4.9 28.6 1.0 CB D:PRO281 4.9 24.4 1.0

M.Suzuki, M.Date, T.Kashiwagi, K.Takahashi, A.Nakamura, M.Tanokura, E.Suzuki, K.Yokoyama. Random Mutagenesis and Disulfide Bond Formation Improved Thermostability in Microbial Transglutaminase. Appl.Microbiol.Biotechnol. 2024.
ISSN: ESSN 1432-0614