Chlorine in PDB 1s4n: Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P

All present enzymatic activity of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P:
2.4.1.131;

The structure of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P, PDB code: 1s4n was solved by Y.D.Lobsanov, P.A.Romero, B.Sleno, B.Yu, P.Yip, A.Herscovics, P.L.Howell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.96 / 2.01
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	60.694, 101.068, 62.036, 90.00, 98.81, 90.00
R / Rfree (%)	18 / 23.2

The binding sites of Chlorine atom in the Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P (pdb code 1s4n). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P, PDB code: 1s4n:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl900 b:23.0 occ:1.00 O A:HOH1155 2.7 38.5 1.0 O B:HOH836 2.9 20.5 1.0 N B:TYR214 3.1 17.6 1.0 N A:TYR419 3.3 16.1 1.0 O A:HOH997 3.3 29.5 1.0 CA B:TYR214 3.5 19.4 1.0 CB A:TYR419 3.6 16.9 1.0 N A:GLY418 3.8 16.3 1.0 CA A:TYR419 4.0 16.6 1.0 CA A:GLY418 4.2 15.2 1.0 C A:GLY418 4.2 17.1 1.0 C B:ILE213 4.2 18.6 1.0 CD1 B:ILE213 4.2 19.7 1.0 CB A:GLN417 4.2 18.3 1.0 CD1 B:TYR214 4.3 23.6 1.0 OE1 A:GLN417 4.3 28.3 1.0 C B:TYR214 4.4 19.0 1.0 CA B:ILE213 4.4 18.2 1.0 O A:HOH1198 4.5 48.0 1.0 C A:GLN417 4.5 15.9 1.0 N B:GLY215 4.5 18.8 1.0 CG B:TYR214 4.7 22.1 1.0 CB B:TYR214 4.7 20.0 1.0 CG1 B:ILE213 4.7 18.7 1.0 O B:TYR212 4.8 21.0 1.0 O B:ALA209 4.8 28.4 1.0 CE1 B:TYR214 4.8 25.2 1.0 CA A:GLN417 4.9 15.7 1.0 CG A:TYR419 4.9 18.6 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl901 b:19.2 occ:1.00 O A:HOH907 3.0 19.8 1.0 N A:TYR214 3.1 16.5 1.0 N B:TYR419 3.3 12.8 1.0 O B:HOH921 3.5 37.0 1.0 CB B:TYR419 3.6 14.9 1.0 CA A:TYR214 3.6 19.1 1.0 N B:GLY418 3.7 12.8 1.0 CD1 A:ILE213 4.0 16.9 1.0 CA B:GLY418 4.1 11.3 1.0 CA B:TYR419 4.1 14.3 1.0 C B:GLY418 4.2 12.8 1.0 CB B:GLN417 4.2 14.7 1.0 C A:ILE213 4.2 17.8 1.0 CD1 A:TYR214 4.3 25.2 1.0 CA A:ILE213 4.4 16.3 1.0 C B:GLN417 4.4 13.9 1.0 OE1 B:GLN417 4.5 21.9 1.0 C A:TYR214 4.5 17.8 1.0 N A:GLY215 4.6 17.3 1.0 CG1 A:ILE213 4.6 16.0 1.0 O A:ALA209 4.7 26.5 1.0 O B:HOH1029 4.8 38.8 1.0 CG A:TYR214 4.8 24.8 1.0 CA B:GLN417 4.8 13.8 1.0 CB A:TYR214 4.8 21.0 1.0 O A:TYR212 4.9 18.7 1.0 CE1 A:TYR214 4.9 28.7 1.0 CG B:TYR419 4.9 15.3 1.0 CE1 B:PHE414 5.0 13.2 1.0

Y.D.Lobsanov, P.A.Romero, B.Sleno, B.Yu, P.Yip, A.Herscovics, P.L.Howell. Structure of KRE2P/MNT1P: A Yeast {Alpha}1,2-Mannosyltransferase Involved in Mannoprotein Biosynthesis J.Biol.Chem. V. 279 17921 2004.
ISSN: ISSN 0021-9258
PubMed: 14752117
DOI: 10.1074/JBC.M312720200