Chlorine in PDB 1t8g: Crystal Structure of Phage T4 Lysozyme Mutant L32A/L33A/T34A/C54T/C97A/E108V

All present enzymatic activity of Crystal Structure of Phage T4 Lysozyme Mutant L32A/L33A/T34A/C54T/C97A/E108V:
3.2.1.17;

The structure of Crystal Structure of Phage T4 Lysozyme Mutant L32A/L33A/T34A/C54T/C97A/E108V, PDB code: 1t8g was solved by M.M.He, Z.A.Wood, W.A.Baase, H.Xiao, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.37 / 1.80
Space group	I 4 2 2
Cell size a, b, c (Å), α, β, γ (°)	96.037, 96.037, 77.265, 90.00, 90.00, 90.00
R / Rfree (%)	18.4 / 21.5

The binding sites of Chlorine atom in the Crystal Structure of Phage T4 Lysozyme Mutant L32A/L33A/T34A/C54T/C97A/E108V (pdb code 1t8g). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Phage T4 Lysozyme Mutant L32A/L33A/T34A/C54T/C97A/E108V, PDB code: 1t8g:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Phage T4 Lysozyme Mutant L32A/L33A/T34A/C54T/C97A/E108V


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Phage T4 Lysozyme Mutant L32A/L33A/T34A/C54T/C97A/E108V within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl400 b:1.0 occ:0.50 NH1 A:ARG137 3.1 13.6 1.0 O A:HOH504 3.3 11.3 1.0 N A:ARG137 3.6 9.7 1.0 CG A:ARG137 3.8 12.4 1.0 CD A:ARG137 4.0 12.6 1.0 CB A:ARG137 4.0 9.3 1.0 CZ A:ARG137 4.3 17.8 1.0 CA A:SER136 4.3 12.0 1.0 C A:SER136 4.5 11.1 1.0 CA A:ARG137 4.5 11.0 1.0 CB A:SER136 4.5 12.3 1.0 O A:HOH550 4.6 16.6 1.0 NE A:ARG137 4.6 13.3 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Phage T4 Lysozyme Mutant L32A/L33A/T34A/C54T/C97A/E108V


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Phage T4 Lysozyme Mutant L32A/L33A/T34A/C54T/C97A/E108V within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl500 b:13.1 occ:1.00 OG A:SER117 3.0 12.5 1.0 O A:HOH509 3.1 17.1 1.0 N A:SER117 3.2 11.4 1.0 C A:PHE114 3.4 10.0 1.0 CA A:PHE114 3.5 10.8 1.0 CB A:SER117 3.6 8.4 1.0 O A:PHE114 3.6 9.8 1.0 N A:ASN116 3.6 10.8 1.0 CB A:PHE114 3.7 11.8 1.0 CD1 A:PHE114 3.8 11.6 1.0 CB A:ASN116 3.9 12.5 1.0 N A:THR115 3.9 11.5 1.0 CA A:SER117 4.0 10.4 1.0 CA A:ASN116 4.1 14.7 1.0 CG A:PHE114 4.1 12.7 1.0 C A:ASN116 4.1 14.1 1.0 CB A:ASN132 4.3 16.5 1.0 C A:THR115 4.4 7.7 1.0 O A:HOH627 4.4 39.7 1.0 O A:ASN132 4.6 12.9 1.0 CA A:THR115 4.7 10.6 1.0 CE1 A:PHE114 4.8 8.8 1.0 CG A:ASN116 4.8 19.8 1.0 C A:ASN132 4.8 14.2 1.0 N A:PHE114 4.9 11.7 1.0 CG A:ASN132 4.9 26.1 1.0 OD1 A:ASN132 4.9 23.1 1.0 O A:GLY113 5.0 8.9 1.0

M.M.He, Z.A.Wood, W.A.Baase, H.Xiao, B.W.Matthews. Alanine-Scanning Mutagenesis of the Beta-Sheet Region of Phage T4 Lysozyme Suggests That Tertiary Context Has A Dominant Effect on Beta-Sheet Formation. Protein Sci. V. 13 2716 2004.
ISSN: ISSN 0961-8368
PubMed: 15340171
DOI: 10.1110/PS.04875504