Chlorine in PDB 1tef: Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond

The structure of Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond, PDB code: 1tef was solved by M.Okvist, F.Jacobson, H.Jansson, O.Hansson, L.Sjolin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.20 / 1.90
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	52.320, 52.320, 127.360, 90.00, 90.00, 120.00
R / Rfree (%)	15.7 / 20.4

The structure of Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond also contains other interesting chemical elements:

Copper

(Cu)

2 atoms

The binding sites of Chlorine atom in the Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond (pdb code 1tef). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond, PDB code: 1tef:

Chlorine binding site 1 out of 1 in the Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl403 b:19.9 occ:1.00 O A:HOH424 3.0 28.1 1.0 N A:ASP51 3.2 14.1 1.0 O A:HOH444 3.4 24.3 1.0 CB A:ASP51 3.8 13.9 1.0 CD A:LYS54 4.0 16.3 1.0 CA A:VAL50 4.0 15.2 1.0 CA A:ASP51 4.0 13.4 1.0 CG1 A:VAL50 4.1 13.5 1.0 C A:VAL50 4.1 14.4 1.0 O A:ASP51 4.2 11.3 1.0 CB A:LYS54 4.3 14.1 1.0 O A:GLY49 4.5 18.0 1.0 C A:ASP51 4.5 11.9 1.0 CB A:VAL50 4.6 15.0 1.0 CG A:LYS54 4.7 15.0 1.0 CG2 A:ILE55 4.7 12.9 1.0 OE2 A:GLU76 4.9 28.1 1.0

M.Okvist, F.Jacobson, H.Jansson, O.Hansson, L.Sjolin. Novel Disulfide Bonds Effect the Thermostability of Plastocyanin. Crystal Structures of the Triple Plastocyanin Mutant G8D/K30C/T69C and the Double Plastocyanin Mutant K30C/T69C From Spinach at 1.90 and 1.96 Resolution, Respectively. To Be Published.