Chlorine in PDB 1znj: Insulin, Monoclinic Crystal Form

The structure of Insulin, Monoclinic Crystal Form, PDB code: 1znj was solved by M.G.W.Turkenburg, J.L.Whittingham, J.P.Turkenburg, G.G.Dodson, U.Derewenda, G.D.Smith, E.J.Dodson, Z.S.Derewenda, B.Xiao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	61.230, 61.650, 48.050, 90.00, 110.50, 90.00
R / Rfree (%)	17.8 / n/a

The structure of Insulin, Monoclinic Crystal Form also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Chlorine atom in the Insulin, Monoclinic Crystal Form (pdb code 1znj). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Insulin, Monoclinic Crystal Form, PDB code: 1znj:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Insulin, Monoclinic Crystal Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Insulin, Monoclinic Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl32 b:21.2 occ:1.00 ZN B:ZN31 2.3 18.0 1.0 NE2 B:HIS10 3.3 7.6 1.0 C3 B:IPH33 3.4 55.9 1.0 NE2 J:HIS10 3.4 15.5 1.0 C4 B:IPH33 3.4 55.9 1.0 NE2 F:HIS10 3.8 21.8 1.0 CD2 B:HIS10 3.8 10.1 1.0 CD1 J:LEU6 3.8 25.7 1.0 CB J:LEU6 3.9 23.4 1.0 CB F:LEU6 3.9 20.1 1.0 CB B:LEU6 3.9 18.0 1.0 CD1 F:LEU6 4.0 20.9 1.0 CD2 F:HIS10 4.1 19.5 1.0 CD2 J:HIS10 4.1 16.7 1.0 CD1 B:LEU6 4.2 21.2 1.0 CE1 J:HIS10 4.4 14.4 1.0 CG J:LEU6 4.4 24.1 1.0 C2 B:IPH33 4.5 55.8 1.0 CG F:LEU6 4.6 20.3 1.0 CG B:LEU6 4.6 19.6 1.0 CE1 B:HIS10 4.6 12.0 1.0 C5 B:IPH33 4.7 56.4 1.0 O J:LEU6 4.7 23.4 1.0 O B:LEU6 4.8 18.2 1.0 C F:LEU6 4.8 20.6 1.0 O F:LEU6 4.8 17.5 1.0 CA F:LEU6 4.8 19.5 1.0 CE1 F:HIS10 4.8 21.5 1.0 C B:LEU6 4.8 17.1 1.0 CA B:LEU6 4.9 17.4 1.0 CA J:LEU6 4.9 23.2 1.0 CD2 J:LEU6 5.0 25.0 1.0 C J:LEU6 5.0 22.8 1.0

Chlorine binding site 2 out of 2 in the Insulin, Monoclinic Crystal Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Insulin, Monoclinic Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ H:Cl31 b:21.1 occ:1.00 ZN D:ZN31 2.3 19.8 1.0 NE2 H:HIS10 3.4 14.2 1.0 NE2 L:HIS10 3.4 13.2 1.0 NE2 D:HIS10 3.7 17.9 1.0 CB L:LEU6 3.7 23.9 1.0 CB H:LEU6 3.9 22.4 1.0 CB D:LEU6 3.9 24.5 1.0 CD2 H:HIS10 3.9 15.2 1.0 CD1 L:LEU6 3.9 24.9 1.0 CD2 D:HIS10 4.0 17.1 1.0 CD2 L:HIS10 4.1 14.2 1.0 O L:HOH46 4.1 73.1 1.0 CG D:LEU6 4.1 26.2 1.0 CD1 H:LEU6 4.3 21.9 1.0 CD2 D:LEU6 4.4 26.1 1.0 CG L:LEU6 4.4 23.8 1.0 CE1 L:HIS10 4.5 15.6 1.0 CE1 H:HIS10 4.6 16.4 1.0 O L:LEU6 4.6 24.1 1.0 CG H:LEU6 4.6 23.4 1.0 O D:LEU6 4.7 20.3 1.0 CE1 D:HIS10 4.8 18.0 1.0 CA L:LEU6 4.8 23.3 1.0 C L:LEU6 4.8 23.4 1.0 C D:LEU6 4.8 22.9 1.0 O H:LEU6 4.8 19.5 1.0 C H:LEU6 4.9 21.5 1.0 CA H:LEU6 4.9 22.7 1.0 CA D:LEU6 4.9 22.9 1.0 CD2 L:LEU6 5.0 23.8 1.0

J.P.Turkenburg, J.L.Whittingham, U.Derewenda, Z.S.Derewenda, E.J.Dodson, G.G.Dodson, G.D.Smith, B.Xiao. Structure Determination and Refinement of Two Crystal Forms of Native Insulins To Be Published.