Chlorine in PDB 226l: Generating Ligand Binding Sites in T4 Lysozyme Using Deficiency-Creating Substitutions

All present enzymatic activity of Generating Ligand Binding Sites in T4 Lysozyme Using Deficiency-Creating Substitutions:
3.2.1.17;

The structure of Generating Ligand Binding Sites in T4 Lysozyme Using Deficiency-Creating Substitutions, PDB code: 226l was solved by E.P.Baldwin, W.A.Baase, X.-J.Zhang, V.Feher, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.80
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	61.200, 61.200, 96.000, 90.00, 90.00, 120.00
R / Rfree (%)	17.5 / n/a

The binding sites of Chlorine atom in the Generating Ligand Binding Sites in T4 Lysozyme Using Deficiency-Creating Substitutions (pdb code 226l). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Generating Ligand Binding Sites in T4 Lysozyme Using Deficiency-Creating Substitutions, PDB code: 226l:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Generating Ligand Binding Sites in T4 Lysozyme Using Deficiency-Creating Substitutions


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Generating Ligand Binding Sites in T4 Lysozyme Using Deficiency-Creating Substitutions within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl173 b:42.3 occ:1.00 O A:HOH209 3.0 36.5 1.0 N A:ARG145 3.3 15.8 1.0 N A:ASN144 3.4 14.4 1.0 C A:THR142 3.4 21.2 1.0 CA A:THR142 3.5 5.5 1.0 O A:THR142 3.6 21.0 1.0 CB A:THR142 3.6 17.7 1.0 CB A:ASN144 3.7 12.8 1.0 CA A:ASN144 3.9 25.2 1.0 N A:PRO143 3.9 25.9 1.0 CB A:ARG145 4.0 21.4 1.0 C A:ASN144 4.1 23.4 1.0 CD A:PRO143 4.1 16.1 1.0 CA A:ARG145 4.2 21.2 1.0 CG2 A:THR142 4.3 13.4 1.0 C A:PRO143 4.3 25.1 1.0 O A:HOH230 4.5 43.7 1.0 CG A:ASN144 4.6 29.9 1.0 CA A:PRO143 4.6 27.4 1.0 ND2 A:ASN144 4.7 21.8 1.0 O A:HOH237 4.7 55.5 1.0 OG1 A:THR142 4.8 21.9 1.0 N A:THR142 4.9 22.6 1.0

Chlorine binding site 2 out of 2 in the Generating Ligand Binding Sites in T4 Lysozyme Using Deficiency-Creating Substitutions


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Generating Ligand Binding Sites in T4 Lysozyme Using Deficiency-Creating Substitutions within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl178 b:41.0 occ:0.50 O A:HOH215 3.0 23.9 1.0 C A:LYS135 3.7 21.3 1.0 CA A:LYS135 3.7 17.0 1.0 N A:SER136 3.9 20.9 1.0 O A:LYS135 4.1 26.2 1.0 OD1 A:ASN140 4.1 22.8 1.0 CB A:LYS135 4.1 19.9 1.0 O A:HOH282 4.2 66.5 1.0 CA A:SER136 4.6 22.5 1.0 C A:SER136 4.8 23.8 1.0 O A:SER136 4.8 28.5 1.0 CG A:LYS135 4.8 15.5 1.0 O A:ALA134 5.0 28.8 1.0

E.Baldwin, W.A.Baase, X.Zhang, V.Feher, B.W.Matthews. Generation of Ligand Binding Sites in T4 Lysozyme By Deficiency-Creating Substitutions. J.Mol.Biol. V. 277 467 1998.
ISSN: ISSN 0022-2836
PubMed: 9514755
DOI: 10.1006/JMBI.1997.1606