Chlorine in PDB 233l: T4 Lysozyme Mutant M120L

All present enzymatic activity of T4 Lysozyme Mutant M120L:
3.2.1.17;

The structure of T4 Lysozyme Mutant M120L, PDB code: 233l was solved by L.A.Lipscomb, D.L.Drew, N.Gassner, W.A.Baase, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.90
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	60.930, 60.930, 97.300, 90.00, 90.00, 120.00
R / Rfree (%)	16.9 / n/a

The binding sites of Chlorine atom in the T4 Lysozyme Mutant M120L (pdb code 233l). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the T4 Lysozyme Mutant M120L, PDB code: 233l:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the T4 Lysozyme Mutant M120L


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of T4 Lysozyme Mutant M120L within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl173 b:19.1 occ:1.00 O A:HOH515 3.1 35.8 1.0 N A:ARG145 3.3 6.9 1.0 N A:ASN144 3.3 3.8 1.0 C A:THR142 3.5 12.7 1.0 CA A:THR142 3.5 5.0 1.0 CB A:THR142 3.6 14.2 1.0 N A:PRO143 3.8 11.2 1.0 O A:THR142 3.8 8.2 1.0 CB A:ASN144 3.8 4.2 1.0 CA A:ASN144 3.8 10.6 1.0 CB A:ARG145 3.9 6.7 1.0 CD A:PRO143 3.9 5.6 1.0 C A:ASN144 4.0 11.8 1.0 CA A:ARG145 4.2 6.7 1.0 C A:PRO143 4.2 9.2 1.0 CG2 A:THR142 4.4 11.4 1.0 ND2 A:ASN144 4.5 33.9 1.0 CA A:PRO143 4.6 12.6 1.0 CG A:ASN144 4.6 20.9 1.0 O A:HOH237 4.7 33.9 1.0 OG1 A:THR142 4.7 15.9 1.0 CG A:PRO143 4.9 6.7 1.0 N A:THR142 4.9 10.7 1.0

Chlorine binding site 2 out of 2 in the T4 Lysozyme Mutant M120L


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of T4 Lysozyme Mutant M120L within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl178 b:34.0 occ:0.50 O A:HOH215 3.0 13.2 1.0 O A:HOH182 3.4 24.1 1.0 O A:HOH501 3.6 32.9 1.0 O A:HOH246 3.9 37.3 1.0 NE2 A:GLN69 4.3 3.2 1.0 CB A:ALA49 4.4 16.0 1.0 CE1 A:HIS31 4.6 8.9 1.0 O A:HOH282 4.7 41.0 1.0 CA A:ALA49 4.7 13.2 1.0

L.A.Lipscomb, N.C.Gassner, S.D.Snow, A.M.Eldridge, W.A.Baase, D.L.Drew, B.W.Matthews. Context-Dependent Protein Stabilization By Methionine-to-Leucine Substitution Shown in T4 Lysozyme. Protein Sci. V. 7 765 1998.
ISSN: ISSN 0961-8368
PubMed: 9541409