Chlorine in PDB 260l: An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme

All present enzymatic activity of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme:
3.2.1.17;

The structure of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme, PDB code: 260l was solved by J.W.Wray, W.A.Baase, G.J.Ostheimer, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.80
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	62.170, 62.170, 96.780, 90.00, 90.00, 120.00
R / Rfree (%)	19 / n/a

The structure of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme also contains other interesting chemical elements:

Nickel

(Ni)

2 atoms

The binding sites of Chlorine atom in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme (pdb code 260l). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme, PDB code: 260l:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl173 b:37.4 occ:1.00 N A:ARG145 3.1 20.1 1.0 N A:ASN144 3.4 16.2 1.0 O A:HOH228 3.4 51.5 1.0 C A:HIS142 3.4 15.2 1.0 CA A:HIS142 3.5 6.6 1.0 O A:HIS142 3.6 16.5 1.0 CB A:HIS142 3.7 7.8 1.0 CB A:ARG145 3.7 19.4 1.0 N A:PRO143 3.8 21.9 1.0 CA A:ASN144 3.8 11.7 1.0 CB A:ASN144 3.9 19.7 1.0 C A:ASN144 3.9 21.9 1.0 CA A:ARG145 4.0 8.3 1.0 CD A:PRO143 4.2 16.8 1.0 C A:PRO143 4.2 12.8 1.0 O A:HOH227 4.3 42.7 1.0 CA A:PRO143 4.5 23.6 1.0 ND2 A:ASN144 4.8 55.6 1.0 CG A:ASN144 4.8 33.7 1.0 CG A:HIS142 4.9 13.8 1.0 N A:HIS142 4.9 14.0 1.0 N A:ALA146 5.0 15.1 1.0

Chlorine binding site 2 out of 2 in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl178 b:27.0 occ:0.50 O A:HOH215 3.0 27.5 1.0 O A:HOH182 3.3 25.8 1.0 O A:HOH200 3.7 27.9 1.0 CB A:ALA49 4.0 11.9 1.0 CE1 A:HIS31 4.2 9.8 1.0 NE2 A:GLN69 4.3 15.0 1.0 NE2 A:HIS31 4.4 19.4 1.0 CA A:ALA49 4.5 16.2 1.0 CD2 A:LEU66 4.6 25.6 1.0 O A:HOH270 4.7 41.4 1.0

J.W.Wray, W.A.Baase, G.J.Ostheimer, X.J.Zhang, B.W.Matthews. Use of A Non-Rigid Region in T4 Lysozyme to Design An Adaptable Metal-Binding Site. Protein Eng. V. 13 313 2000.
ISSN: ISSN 0269-2139
PubMed: 10835104
DOI: 10.1093/PROTEIN/13.5.313