Chlorine in PDB 2cim: Crystal Structure of Methanosarcina Barkeri Seryl-Trna Synthetase

All present enzymatic activity of Crystal Structure of Methanosarcina Barkeri Seryl-Trna Synthetase:
6.1.1.11;

The structure of Crystal Structure of Methanosarcina Barkeri Seryl-Trna Synthetase, PDB code: 2cim was solved by S.Bilokapic, T.Maier, D.Ahel, I.Gruic-Sovulj, D.Soll, I.Weygand-Durasevic, N.Ban, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.94 / 2.51
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	97.150, 97.150, 268.500, 90.00, 90.00, 120.00
R / Rfree (%)	19.9 / 25.2

The structure of Crystal Structure of Methanosarcina Barkeri Seryl-Trna Synthetase also contains other interesting chemical elements:

Platinum	(Pt)	1 atom
Zinc	(Zn)	2 atoms

The binding sites of Chlorine atom in the Crystal Structure of Methanosarcina Barkeri Seryl-Trna Synthetase (pdb code 2cim). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Methanosarcina Barkeri Seryl-Trna Synthetase, PDB code: 2cim:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Methanosarcina Barkeri Seryl-Trna Synthetase


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Methanosarcina Barkeri Seryl-Trna Synthetase within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1504 b:38.8 occ:1.00 N A:ALA304 3.1 63.0 1.0 NE1 A:TRP245 3.2 66.0 1.0 N A:TYR303 3.2 62.2 1.0 N A:GLN305 3.3 64.1 1.0 CA A:TYR303 3.8 62.1 1.0 CZ2 A:TRP245 3.8 65.0 1.0 CE2 A:TRP245 3.8 65.3 1.0 CB A:TYR303 3.9 62.0 1.0 C A:TYR303 3.9 62.6 1.0 CD A:PRO307 3.9 64.6 1.0 CG A:PRO307 3.9 63.9 1.0 CA A:ALA304 3.9 63.4 1.0 CG2 A:VAL240 4.0 63.0 1.0 CD A:PRO308 4.0 63.6 1.0 C A:ALA304 4.1 63.8 1.0 CB A:ALA304 4.2 62.5 1.0 C A:CYS302 4.2 63.0 1.0 CA A:GLN305 4.2 64.6 1.0 CB A:PRO307 4.2 64.0 1.0 CG1 A:VAL240 4.3 62.4 1.0 CA A:CYS302 4.3 63.2 1.0 CD1 A:TRP245 4.3 64.9 1.0 CB A:VAL240 4.6 63.0 1.0 SG A:CYS302 4.8 63.7 1.0 C A:GLN305 4.8 64.8 1.0 CG A:PRO308 4.8 63.7 1.0 N A:PRO307 4.9 64.5 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Methanosarcina Barkeri Seryl-Trna Synthetase


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Methanosarcina Barkeri Seryl-Trna Synthetase within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1504 b:43.3 occ:1.00 N B:ALA304 3.1 63.3 1.0 NE1 B:TRP245 3.2 61.6 1.0 N B:TYR303 3.3 62.2 1.0 N B:GLN305 3.5 64.4 1.0 CG B:PRO307 3.6 64.7 1.0 CG2 B:VAL240 3.8 62.7 1.0 CD B:PRO307 3.8 64.8 1.0 CA B:TYR303 3.9 62.5 1.0 CE2 B:TRP245 3.9 62.4 1.0 CA B:ALA304 3.9 63.5 1.0 CD B:PRO308 4.0 63.1 1.0 C B:TYR303 4.0 63.0 1.0 CZ2 B:TRP245 4.0 62.1 1.0 CB B:PRO307 4.0 64.7 1.0 CB B:TYR303 4.1 62.7 1.0 CB B:ALA304 4.1 63.0 1.0 CG1 B:VAL240 4.1 60.9 1.0 C B:ALA304 4.2 63.8 1.0 C B:CYS302 4.3 62.5 1.0 CD1 B:TRP245 4.3 62.3 1.0 CA B:CYS302 4.4 62.4 1.0 CA B:GLN305 4.4 64.6 1.0 CB B:VAL240 4.4 61.7 1.0 CG B:PRO308 4.7 63.4 1.0 N B:PRO307 4.8 65.0 1.0 C B:GLN305 4.8 64.6 1.0 SG B:CYS302 4.8 62.6 1.0

S.Bilokapic, T.Maier, D.Ahel, I.Gruic-Sovulj, D.Soll, I.Weygand-Durasevic, N.Ban. Structure of the Unusual Seryl-Trna Synthetase Reveals A Distinct Zinc-Dependent Mode of Substrate Recognition Embo J. V. 25 2498 2006.
ISSN: ISSN 0261-4189
PubMed: 16675947
DOI: 10.1038/SJ.EMBOJ.7601129