Chlorine in PDB 2flc: Post-Reactive Complex of Restriction Endonuclease HINP1I with Nicked Cognate Dna and Magnesium Ions

All present enzymatic activity of Post-Reactive Complex of Restriction Endonuclease HINP1I with Nicked Cognate Dna and Magnesium Ions:
3.1.21.4;

The structure of Post-Reactive Complex of Restriction Endonuclease HINP1I with Nicked Cognate Dna and Magnesium Ions, PDB code: 2flc was solved by J.R.Horton, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.10 / 2.59
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	100.387, 100.387, 139.369, 90.00, 90.00, 120.00
R / Rfree (%)	27.5 / 32.4

The structure of Post-Reactive Complex of Restriction Endonuclease HINP1I with Nicked Cognate Dna and Magnesium Ions also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Chlorine atom in the Post-Reactive Complex of Restriction Endonuclease HINP1I with Nicked Cognate Dna and Magnesium Ions (pdb code 2flc). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Post-Reactive Complex of Restriction Endonuclease HINP1I with Nicked Cognate Dna and Magnesium Ions, PDB code: 2flc:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Post-Reactive Complex of Restriction Endonuclease HINP1I with Nicked Cognate Dna and Magnesium Ions


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Post-Reactive Complex of Restriction Endonuclease HINP1I with Nicked Cognate Dna and Magnesium Ions within 5.0Å range: probe atom residue distance (Å) B Occ E:Cl4 b:72.0 occ:1.00 N7 E:DG19 3.7 73.8 1.0 O6 E:DG20 3.9 60.5 1.0 O6 E:DG19 3.9 72.0 1.0 C7 E:DT18 4.1 88.1 1.0 C5 E:DG19 4.3 73.1 1.0 N4 C:DC1 4.4 75.8 1.0 C6 E:DG19 4.4 72.3 1.0 C5 E:DT18 4.4 87.6 1.0 C8 E:DG19 4.6 74.9 1.0 C4 E:DT18 4.9 87.2 1.0 C6 E:DT18 4.9 87.8 1.0 C6 E:DG20 5.0 58.9 1.0

Chlorine binding site 2 out of 2 in the Post-Reactive Complex of Restriction Endonuclease HINP1I with Nicked Cognate Dna and Magnesium Ions


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Post-Reactive Complex of Restriction Endonuclease HINP1I with Nicked Cognate Dna and Magnesium Ions within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl249 b:56.5 occ:1.00 NE2 A:GLN198 3.5 57.1 1.0 OE1 A:GLN198 3.7 59.2 1.0 CB A:SER202 3.7 56.9 1.0 CA A:SER202 3.8 57.9 1.0 N A:SER202 3.9 57.5 1.0 CD A:GLN198 4.0 56.4 1.0 O A:GLN198 4.2 50.8 1.0 C A:GLY201 4.6 57.0 1.0 OG A:SER202 4.8 55.5 1.0

J.R.Horton, X.Zhang, R.Maunus, Z.Yang, G.G.Wilson, R.J.Roberts, X.Cheng. Dna Nicking By HINP1I Endonuclease: Bending, Base Flipping and Minor Groove Expansion. Nucleic Acids Res. V. 34 939 2006.
ISSN: ISSN 0305-1048
PubMed: 16473850
DOI: 10.1093/NAR/GKJ484