Chlorine in PDB 2gws: Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus

Enzymatic activity of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus

All present enzymatic activity of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus:
2.7.7.7;

Protein crystallography data

The structure of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus, PDB code: 2gws was solved by M.Garcia-Diaz, A.J.Picher, K.Bebenek, L.C.Pedersen, T.A.Kunkel, L.Blanco, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	50.00 / 2.40
*Space group*	P 2₁ 2₁ 2
*Cell size a, b, c (Å), α, β, γ (°)*	192.374, 98.268, 104.960, 90.00, 90.00, 90.00
*R / R_free (%)*	20.9 / 25.3

Other elements in 2gws:

The structure of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus also contains other interesting chemical elements:

Magnesium	(Mg)	1 atom
Arsenic	(As)	3 atoms
Sodium	(Na)	8 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus (pdb code 2gws). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus, PDB code: 2gws:

Chlorine binding site 1 out of 1 in 2gws

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Chlorine Binding Sites List in 2gws

Chlorine binding site 1 out of 1 in the Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus

Mono view

Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Cl952 b:41.8 occ:1.00	N	A:GLU556	3.1	22.3	1.0
	O	A:HOH975	3.2	26.8	1.0
	CB	A:THR555	3.5	19.9	1.0
	CA	A:THR555	3.5	20.2	1.0
	C	A:THR555	3.8	22.4	1.0
	CG	A:GLU556	3.8	31.6	1.0
	CD2	A:LEU503	3.9	33.5	1.0
	O	A:HOH1139	3.9	59.8	1.0
	CB	A:GLU556	4.0	26.4	1.0
	CA	A:GLU556	4.1	26.4	1.0
	CG2	A:THR555	4.3	14.0	1.0
	CD	A:GLU556	4.6	34.0	1.0
	OG1	A:THR555	4.6	20.9	1.0
	OE2	A:GLU556	4.6	34.2	1.0
	O	A:PRO554	4.8	23.7	1.0
	CB	A:ALA500	4.9	19.7	1.0
	N	A:THR555	4.9	21.0	1.0
	CA	A:ALA500	4.9	22.4	1.0

Reference:

A.J.Picher, M.Garcia-Diaz, K.Bebenek, L.C.Pedersen, T.A.Kunkel, L.Blanco. Promiscuous Mismatch Extension By Human Dna Polymerase Lambda. Nucleic Acids Res. V. 34 3259 2006.
ISSN: ISSN 0305-1048
PubMed: 16807316
DOI: 10.1093/NAR/GKL377

Page generated: Sat Jul 20 07:41:00 2024

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