Chlorine in PDB 2o54: Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol at pH 7.0

All present enzymatic activity of Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol at pH 7.0:
5.99.1.2;

The structure of Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol at pH 7.0, PDB code: 2o54 was solved by A.Changela, R.J.Digate, A.Mondragon, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.10 / 2.50
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	101.830, 101.830, 452.380, 90.00, 90.00, 90.00
R / Rfree (%)	22.2 / 26.9

The binding sites of Chlorine atom in the Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol at pH 7.0 (pdb code 2o54). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol at pH 7.0, PDB code: 2o54:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol at pH 7.0


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol at pH 7.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl800 b:23.8 occ:1.00 NH1 A:ARG185 3.3 24.4 1.0 N4 C:DC1 3.5 27.8 1.0 CD A:ARG185 4.1 26.4 1.0 CG A:ARG185 4.1 26.2 1.0 CZ A:ARG185 4.4 24.8 1.0 CB A:ARG185 4.5 27.8 1.0 C4 C:DC1 4.5 27.8 1.0 N3 C:DC1 4.6 28.7 1.0 NE A:ARG185 4.8 26.6 1.0 NH1 A:ARG60 4.8 30.0 1.0

Chlorine binding site 2 out of 2 in the Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol at pH 7.0


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol at pH 7.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl801 b:20.9 occ:1.00 NH1 A:ARG60 3.2 30.0 1.0 N A:TRP61 3.3 24.2 1.0 N4 C:DC1 3.4 27.8 1.0 CD A:ARG60 3.7 28.7 1.0 C5 C:DC1 4.0 26.4 1.0 CA A:ARG60 4.0 25.1 1.0 C4 C:DC1 4.1 27.8 1.0 CB A:TRP61 4.1 23.1 1.0 C A:ARG60 4.1 24.5 1.0 CZ A:ARG60 4.2 30.9 1.0 CA A:TRP61 4.2 23.5 1.0 O A:ALA59 4.4 25.0 1.0 CD1 A:TRP61 4.4 22.2 1.0 O A:TRP61 4.4 24.9 1.0 NE A:ARG60 4.4 31.3 1.0 CG A:TRP61 4.4 22.6 1.0 CG A:ARG60 4.5 25.6 1.0 CB A:ARG60 4.7 26.8 1.0 C A:TRP61 4.8 24.0 1.0

A.Changela, R.J.Digate, A.Mondragon. Structural Studies of E. Coli Topoisomerase III-Dna Complexes Reveal A Novel Type Ia Topoisomerase-Dna Conformational Intermediate. J.Mol.Biol. V. 368 105 2007.
ISSN: ISSN 0022-2836
PubMed: 17331537
DOI: 10.1016/J.JMB.2007.01.065