Chlorine in PDB 2o59: Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol pH 8.0

All present enzymatic activity of Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol pH 8.0:
5.99.1.2;

The structure of Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol pH 8.0, PDB code: 2o59 was solved by A.Changela, R.J.Digate, A.Mondragon, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.53 / 2.50
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	101.590, 101.590, 453.590, 90.00, 90.00, 90.00
R / Rfree (%)	22.1 / 25.8

The binding sites of Chlorine atom in the Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol pH 8.0 (pdb code 2o59). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol pH 8.0, PDB code: 2o59:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol pH 8.0


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol pH 8.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl800 b:37.9 occ:1.00 N4 C:DC1 3.2 42.6 1.0 N A:TRP61 3.2 37.6 1.0 NH1 A:ARG60 3.3 40.7 1.0 CD A:ARG60 3.7 38.0 1.0 CB A:TRP61 3.8 37.4 1.0 C5 C:DC1 3.9 42.7 1.0 C4 C:DC1 4.0 42.8 1.0 CA A:ARG60 4.0 38.2 1.0 CA A:TRP61 4.1 38.0 1.0 C A:ARG60 4.1 38.0 1.0 CG A:TRP61 4.2 37.1 1.0 CD1 A:TRP61 4.2 37.4 1.0 O A:ALA59 4.2 40.1 1.0 CZ A:ARG60 4.3 40.4 1.0 O A:TRP61 4.4 39.6 1.0 NE A:ARG60 4.4 39.9 1.0 CG A:ARG60 4.5 37.1 1.0 C A:TRP61 4.7 38.9 1.0 CB A:ARG60 4.7 38.8 1.0

Chlorine binding site 2 out of 2 in the Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol pH 8.0


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glycerol pH 8.0 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl801 b:39.0 occ:1.00 NH1 B:ARG60 3.1 44.6 1.0 N4 D:DC1 3.1 37.7 1.0 N B:TRP61 3.3 41.9 1.0 C5 D:DC1 3.8 38.5 1.0 CD B:ARG60 3.8 44.0 1.0 C4 D:DC1 3.9 37.7 1.0 CB B:TRP61 3.9 40.7 1.0 CA B:ARG60 4.1 44.3 1.0 CA B:TRP61 4.2 40.9 1.0 C B:ARG60 4.2 42.4 1.0 CZ B:ARG60 4.2 46.5 1.0 O B:TRP61 4.3 41.7 1.0 CG B:TRP61 4.3 41.0 1.0 O B:ALA59 4.4 45.8 1.0 CD1 B:TRP61 4.4 40.7 1.0 NE B:ARG60 4.5 45.8 1.0 CG B:ARG60 4.5 43.9 1.0 C B:TRP61 4.7 40.9 1.0 CB B:ARG60 4.8 45.1 1.0

A.Changela, R.J.Digate, A.Mondragon. Structural Studies of E. Coli Topoisomerase III-Dna Complexes Reveal A Novel Type Ia Topoisomerase-Dna Conformational Intermediate. J.Mol.Biol. V. 368 105 2007.
ISSN: ISSN 0022-2836
PubMed: 17331537
DOI: 10.1016/J.JMB.2007.01.065