Chlorine in PDB 2o5e: Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glucose pH 7.0

All present enzymatic activity of Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glucose pH 7.0:
5.99.1.2;

The structure of Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glucose pH 7.0, PDB code: 2o5e was solved by A.Changela, R.J.Digate, A.Mondragon, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.13 / 2.50
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	102.260, 102.260, 445.610, 90.00, 90.00, 90.00
R / Rfree (%)	21.7 / 26

The binding sites of Chlorine atom in the Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glucose pH 7.0 (pdb code 2o5e). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glucose pH 7.0, PDB code: 2o5e:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glucose pH 7.0


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glucose pH 7.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl800 b:49.2 occ:1.00 NH1 A:ARG185 3.3 42.6 1.0 N4 C:DC1 3.7 41.4 1.0 CD A:ARG185 3.7 46.6 1.0 CG A:ARG185 4.1 48.9 1.0 N3 C:DC1 4.3 42.2 1.0 CZ A:ARG185 4.3 43.8 1.0 C4 C:DC1 4.4 42.1 1.0 NE A:ARG185 4.5 44.5 1.0 CB A:ARG185 4.5 50.5 1.0 NH1 A:ARG60 4.9 52.3 1.0

Chlorine binding site 2 out of 2 in the Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glucose pH 7.0


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of E. Coli Topoisomerase III in Complex with An 8-Base Single Stranded Oligonucleotide. Frozen in Glucose pH 7.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl801 b:52.0 occ:1.00 N4 C:DC1 3.0 41.4 1.0 NH1 A:ARG60 3.0 52.3 1.0 N A:TRP61 3.2 47.7 1.0 CD A:ARG60 3.6 51.7 1.0 CB A:TRP61 3.8 45.8 1.0 C4 C:DC1 3.9 42.1 1.0 C5 C:DC1 3.9 41.8 1.0 CA A:ARG60 3.9 49.6 1.0 CA A:TRP61 4.0 46.8 1.0 C A:ARG60 4.1 48.8 1.0 CZ A:ARG60 4.1 55.0 1.0 CG A:TRP61 4.2 44.5 1.0 CD1 A:TRP61 4.3 44.3 1.0 NE A:ARG60 4.3 54.5 1.0 O A:TRP61 4.3 47.9 1.0 O A:ALA59 4.4 48.5 1.0 CG A:ARG60 4.4 50.4 1.0 C A:TRP61 4.6 47.8 1.0 CB A:ARG60 4.7 50.8 1.0

A.Changela, R.J.Digate, A.Mondragon. Structural Studies of E. Coli Topoisomerase III-Dna Complexes Reveal A Novel Type Ia Topoisomerase-Dna Conformational Intermediate. J.Mol.Biol. V. 368 105 2007.
ISSN: ISSN 0022-2836
PubMed: 17331537
DOI: 10.1016/J.JMB.2007.01.065