Chlorine in PDB 3dic: Crystal Structure of Bovine Pancreatic Ribonuclease A Variant (V108A)

All present enzymatic activity of Crystal Structure of Bovine Pancreatic Ribonuclease A Variant (V108A):
3.1.27.5;

The structure of Crystal Structure of Bovine Pancreatic Ribonuclease A Variant (V108A), PDB code: 3dic was solved by K.Kurpiewska, J.Font, M.Ribo, M.Vilanova, K.Lewinski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.60
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	63.985, 63.985, 63.663, 90.00, 90.00, 120.00
R / Rfree (%)	18.8 / 21

The binding sites of Chlorine atom in the Crystal Structure of Bovine Pancreatic Ribonuclease A Variant (V108A) (pdb code 3dic). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Crystal Structure of Bovine Pancreatic Ribonuclease A Variant (V108A), PDB code: 3dic:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Crystal Structure of Bovine Pancreatic Ribonuclease A Variant (V108A)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Bovine Pancreatic Ribonuclease A Variant (V108A) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl125 b:18.0 occ:1.00 ND2 A:ASN34 3.0 26.9 1.0 CD A:ARG10 3.4 9.4 1.0 NE A:ARG10 3.8 10.4 1.0 CG A:ASN34 3.9 22.7 1.0 CB A:ASN34 3.9 20.4 1.0 CZ A:ARG10 4.2 12.1 1.0 NH1 A:ARG10 4.3 12.5 1.0 O A:HOH1109 4.6 52.3 1.0 O A:HOH1136 4.6 47.7 1.0 O A:ARG33 4.8 11.8 1.0 CG A:ARG10 4.8 7.4 1.0

Chlorine binding site 2 out of 3 in the Crystal Structure of Bovine Pancreatic Ribonuclease A Variant (V108A)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Bovine Pancreatic Ribonuclease A Variant (V108A) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl126 b:12.6 occ:1.00 OG1 A:THR3 3.0 9.2 1.0 O A:HOH1096 3.1 15.2 1.0 O A:HOH1006 3.3 10.0 1.0 CB A:THR3 4.0 7.5 1.0 CG2 A:THR3 4.1 11.3 1.0 CB A:ALA5 4.2 8.4 1.0 N A:ALA6 4.2 7.3 1.0 O A:HOH1101 4.2 12.6 1.0 CB A:ALA6 4.2 7.6 1.0 CA A:ALA6 4.7 6.9 1.0 C A:ALA5 4.9 9.1 1.0 O A:HOH1112 4.9 12.0 1.0 O A:HOH1021 4.9 14.0 1.0 CA A:ALA5 5.0 5.9 1.0

Chlorine binding site 3 out of 3 in the Crystal Structure of Bovine Pancreatic Ribonuclease A Variant (V108A)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of Bovine Pancreatic Ribonuclease A Variant (V108A) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl127 b:15.3 occ:1.00 O A:HOH1200 2.7 48.0 1.0 O A:HOH1081 2.9 38.6 1.0 N A:THR45 3.3 8.9 1.0 CE1 A:HIS12 3.4 10.0 1.0 O A:HOH1170 3.5 33.0 1.0 CA A:ASN44 3.7 9.0 1.0 OG1 A:THR45 3.8 11.1 1.0 CD1 A:PHE120 3.9 11.4 1.0 C A:ASN44 3.9 7.0 1.0 CB A:THR45 4.0 10.6 1.0 ND1 A:HIS12 4.1 7.7 1.0 CA A:THR45 4.3 7.2 1.0 CB A:PHE120 4.3 11.9 1.0 O A:VAL43 4.3 11.6 1.0 OD1 A:ASN44 4.3 11.5 1.0 N A:ASN44 4.3 9.0 1.0 CG A:PHE120 4.3 10.5 1.0 NE2 A:HIS12 4.4 8.7 1.0 O A:PHE120 4.6 11.9 1.0 NZ A:LYS41 4.6 23.1 1.0 C A:VAL43 4.6 10.7 1.0 CG1 A:VAL43 4.6 12.7 1.0 O A:HOH1118 4.7 47.3 1.0 CE1 A:PHE120 4.7 15.4 1.0 CG A:ASN44 4.8 13.5 1.0 CB A:ASN44 4.8 9.8 1.0 O A:THR45 4.8 8.8 1.0

K.Kurpiewska, J.Font, M.Ribo, M.Vilanova, K.Lewinski. X-Ray Crystallographic Studies of Rnase A Variants Engineered at the Most Destabilizing Positions of the Main Hydrophobic Core: Further Insight Into Protein Stability Proteins V. 77 658 2009.
ISSN: ISSN 0887-3585
PubMed: 19544568
DOI: 10.1002/PROT.22480