Chlorine in PDB 3ojp: D52N Mutant of Hen Egg White Lysozyme (Hewl)

All present enzymatic activity of D52N Mutant of Hen Egg White Lysozyme (Hewl):
3.2.1.17;

The structure of D52N Mutant of Hen Egg White Lysozyme (Hewl), PDB code: 3ojp was solved by F.O'meara, J.Bradley, P.E.O'rourke, H.Webb, B.M.Tynan-Connolly, J.E.Nielsen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.22 / 1.81
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	78.760, 78.760, 36.950, 90.00, 90.00, 90.00
R / Rfree (%)	16.7 / 21.9

The structure of D52N Mutant of Hen Egg White Lysozyme (Hewl) also contains other interesting chemical elements:

Sodium

(Na)

1 atom

The binding sites of Chlorine atom in the D52N Mutant of Hen Egg White Lysozyme (Hewl) (pdb code 3ojp). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the D52N Mutant of Hen Egg White Lysozyme (Hewl), PDB code: 3ojp:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the D52N Mutant of Hen Egg White Lysozyme (Hewl)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of D52N Mutant of Hen Egg White Lysozyme (Hewl) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl133 b:23.1 occ:1.00 O A:HOH206 2.7 41.8 1.0 OG A:SER24 3.0 15.1 1.0 N A:GLY26 3.1 12.0 1.0 CB A:SER24 3.5 14.9 1.0 CA A:GLY26 3.5 12.3 1.0 O A:HOH262 3.5 50.0 1.0 CA A:GLN121 3.6 19.8 1.0 N A:GLN121 3.9 18.5 1.0 CB A:GLN121 4.0 22.0 1.0 CD1 A:ILE124 4.1 23.6 1.0 CG1 A:ILE124 4.2 17.7 1.0 N A:LEU25 4.2 13.9 1.0 C A:VAL120 4.3 17.0 1.0 CG A:GLN121 4.3 27.2 1.0 C A:SER24 4.3 14.5 1.0 C A:LEU25 4.3 13.8 1.0 O A:VAL120 4.4 15.8 1.0 CG2 A:VAL120 4.4 14.5 1.0 CA A:SER24 4.5 14.3 1.0 C A:GLY26 4.6 12.2 1.0 N A:ASN27 4.7 11.6 1.0 CA A:LEU25 4.7 13.9 1.0 C A:GLN121 4.8 18.6 1.0 O A:SER24 4.8 13.9 1.0 O A:GLN121 5.0 18.6 1.0

Chlorine binding site 2 out of 4 in the D52N Mutant of Hen Egg White Lysozyme (Hewl)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of D52N Mutant of Hen Egg White Lysozyme (Hewl) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl134 b:32.7 occ:1.00 CE2 A:PHE38 3.7 16.6 1.0 NZ A:LYS33 3.8 21.3 1.0 CZ3 A:TRP123 3.8 17.1 1.0 CE A:LYS33 4.1 22.5 1.0 CD A:ARG5 4.2 21.8 1.0 CB A:ARG5 4.3 17.6 1.0 CD A:LYS33 4.3 15.7 1.0 CZ A:PHE38 4.4 14.3 1.0 CG A:ARG5 4.6 17.1 1.0 CD2 A:PHE38 4.7 16.9 1.0 CE3 A:TRP123 4.7 16.3 1.0 O A:HOH207 4.7 37.7 1.0 CH2 A:TRP123 4.7 18.4 1.0 NE A:ARG5 4.8 23.5 1.0

Chlorine binding site 3 out of 4 in the D52N Mutant of Hen Egg White Lysozyme (Hewl)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of D52N Mutant of Hen Egg White Lysozyme (Hewl) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl135 b:16.4 occ:1.00 OH A:TYR23 3.1 13.3 1.0 CZ A:TYR23 3.7 9.3 1.0 CE2 A:TYR23 3.7 9.6 1.0 CA A:GLY104 4.2 13.1 1.0 O A:ARG21 4.5 14.3 1.0 N A:GLY104 4.7 13.0 1.0 CD2 A:TYR23 4.9 11.9 1.0 CE1 A:TYR23 4.9 11.3 1.0

Chlorine binding site 4 out of 4 in the D52N Mutant of Hen Egg White Lysozyme (Hewl)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of D52N Mutant of Hen Egg White Lysozyme (Hewl) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl136 b:18.9 occ:1.00 O A:HOH149 3.1 10.7 1.0 O A:HOH213 3.2 23.8 1.0 N A:THR69 3.2 14.7 1.0 O A:HOH243 3.4 27.9 1.0 O A:THR69 3.4 14.5 1.0 N A:ARG68 3.5 15.4 1.0 C A:GLY67 3.5 16.3 1.0 CA A:GLY67 3.6 15.2 1.0 OD1 A:ASN65 3.6 16.4 1.0 N A:GLY67 3.7 15.6 1.0 OG A:SER72 3.7 19.0 1.0 C A:THR69 3.7 15.8 1.0 CA A:THR69 3.9 14.0 1.0 O A:HOH161 4.1 12.4 1.0 O A:GLY67 4.2 16.7 1.0 C A:ARG68 4.2 15.6 1.0 CA A:ARG68 4.3 16.3 0.7 CA A:ARG68 4.3 16.1 0.3 CB A:THR69 4.3 14.7 1.0 OD1 A:ASP66 4.3 13.2 1.0 N A:PRO70 4.6 17.9 1.0 NA A:NA137 4.7 17.8 1.0 C A:ASP66 4.8 14.1 1.0 O A:HOH194 4.8 25.3 0.5 OG1 A:THR69 4.8 12.1 1.0 CG A:ASN65 4.8 14.8 1.0 N A:ASP66 4.9 12.6 1.0 CB A:SER72 5.0 21.2 1.0

F.O'meara, J.Bradley, P.E.O'rourke, H.Webb, B.M.Tynan-Connolly, J.E.Nielsen. D52N Mutant of Hen Egg White Lysozyme (Hewl) To Be Published.