Chlorine in PDB 3rpl: D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate
Enzymatic activity of D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate
All present enzymatic activity of D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate:
3.1.3.11;
3.1.3.37;
Protein crystallography data
The structure of D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate, PDB code: 3rpl
was solved by
X.Hu,
D.Hui,
F.Lingling,
W.Jian,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
25.72 /
2.40
|
Space group
|
P 65
|
Cell size a, b, c (Å), α, β, γ (°)
|
145.222,
145.222,
169.104,
90.00,
90.00,
120.00
|
R / Rfree (%)
|
16.6 /
20.7
|
Other elements in 3rpl:
The structure of D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate also contains other interesting chemical elements:
Chlorine Binding Sites:
The binding sites of Chlorine atom in the D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate
(pdb code 3rpl). This binding sites where shown within
5.0 Angstroms radius around Chlorine atom.
In total 6 binding sites of Chlorine where determined in the
D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate, PDB code: 3rpl:
Jump to Chlorine binding site number:
1;
2;
3;
4;
5;
6;
Chlorine binding site 1 out
of 6 in 3rpl
Go back to
Chlorine Binding Sites List in 3rpl
Chlorine binding site 1 out
of 6 in the D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate
 Mono view
 Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 1 of D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cl354
b:50.2
occ:1.00
|
NH1
|
A:ARG60
|
3.9
|
24.7
|
1.0
|
NH2
|
A:ARG60
|
4.0
|
23.1
|
1.0
|
CZ
|
A:ARG60
|
4.4
|
28.4
|
1.0
|
|
Chlorine binding site 2 out
of 6 in 3rpl
Go back to
Chlorine Binding Sites List in 3rpl
Chlorine binding site 2 out
of 6 in the D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate
 Mono view
 Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 2 of D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Cl354
b:37.0
occ:1.00
|
CB
|
C:CYS281
|
3.3
|
29.8
|
1.0
|
NZ
|
C:LYS142
|
3.3
|
36.5
|
1.0
|
N
|
C:PHE240
|
3.3
|
21.7
|
1.0
|
O
|
C:PHE240
|
3.4
|
20.8
|
1.0
|
N
|
C:CYS281
|
3.5
|
24.5
|
1.0
|
C
|
C:PHE240
|
3.9
|
20.8
|
1.0
|
CB
|
C:ASN280
|
3.9
|
29.7
|
1.0
|
ND1
|
C:HIS239
|
4.0
|
17.1
|
1.0
|
CA
|
C:HIS239
|
4.0
|
17.3
|
1.0
|
CA
|
C:CYS281
|
4.1
|
27.4
|
1.0
|
C
|
C:HIS239
|
4.2
|
20.6
|
1.0
|
CG
|
C:HIS239
|
4.2
|
20.4
|
1.0
|
CA
|
C:PHE240
|
4.2
|
20.6
|
1.0
|
CE1
|
C:HIS239
|
4.2
|
19.5
|
1.0
|
OE2
|
C:GLU282
|
4.3
|
35.4
|
1.0
|
CA
|
C:ASN280
|
4.4
|
26.8
|
1.0
|
O
|
C:GLY238
|
4.4
|
21.4
|
1.0
|
OE1
|
C:GLU282
|
4.4
|
51.2
|
1.0
|
CG
|
C:GLN241
|
4.5
|
22.5
|
1.0
|
C
|
C:ASN280
|
4.5
|
26.2
|
1.0
|
CD2
|
C:HIS239
|
4.5
|
19.1
|
1.0
|
NE2
|
C:HIS239
|
4.5
|
17.2
|
1.0
|
SG
|
C:CYS281
|
4.6
|
35.7
|
1.0
|
CE
|
C:LYS142
|
4.6
|
36.2
|
1.0
|
CG
|
C:LYS142
|
4.6
|
23.5
|
1.0
|
CB
|
C:HIS239
|
4.7
|
19.1
|
1.0
|
CG
|
C:ASN280
|
4.7
|
40.3
|
1.0
|
N
|
C:GLN241
|
4.7
|
18.4
|
1.0
|
CD
|
C:GLU282
|
4.8
|
52.4
|
1.0
|
|
Chlorine binding site 3 out
of 6 in 3rpl
Go back to
Chlorine Binding Sites List in 3rpl
Chlorine binding site 3 out
of 6 in the D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate
 Mono view
 Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 3 of D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Cl355
b:45.0
occ:1.00
|
O2
|
C:GOL358
|
3.6
|
36.5
|
1.0
|
CG1
|
C:ILE220
|
3.9
|
26.9
|
1.0
|
CD1
|
C:ILE220
|
3.9
|
29.4
|
1.0
|
CG
|
C:LYS133
|
4.3
|
27.2
|
1.0
|
CD
|
C:LYS133
|
4.3
|
27.5
|
1.0
|
CE
|
C:LYS133
|
4.4
|
33.3
|
1.0
|
CA
|
C:GLY219
|
4.5
|
19.8
|
1.0
|
C
|
C:GLY219
|
4.7
|
20.1
|
1.0
|
N
|
C:ILE220
|
4.7
|
23.4
|
1.0
|
O3
|
C:GOL358
|
4.9
|
33.8
|
1.0
|
CB
|
C:LYS133
|
4.9
|
25.4
|
1.0
|
CD2
|
C:LEU182
|
5.0
|
18.7
|
1.0
|
|
Chlorine binding site 4 out
of 6 in 3rpl
Go back to
Chlorine Binding Sites List in 3rpl
Chlorine binding site 4 out
of 6 in the D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate
 Mono view
 Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 4 of D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Cl356
b:65.3
occ:1.00
|
O
|
A:HOH451
|
3.3
|
30.6
|
1.0
|
O
|
B:HOH494
|
3.4
|
36.1
|
1.0
|
O
|
A:HOH426
|
3.6
|
24.0
|
1.0
|
O
|
A:HOH560
|
3.7
|
28.7
|
1.0
|
O
|
B:HOH406
|
3.7
|
29.2
|
1.0
|
O
|
B:HOH467
|
3.8
|
26.2
|
1.0
|
OG
|
B:SER210
|
3.8
|
25.1
|
1.0
|
O
|
B:HOH490
|
4.3
|
26.8
|
1.0
|
CB
|
B:SER210
|
4.4
|
18.2
|
1.0
|
NE2
|
C:GLN343
|
4.6
|
18.6
|
1.0
|
O
|
A:HOH666
|
4.6
|
33.9
|
1.0
|
CG1
|
C:VAL341
|
4.7
|
20.2
|
1.0
|
O1P
|
A:AMP349
|
4.7
|
16.1
|
1.0
|
CG2
|
C:VAL341
|
4.8
|
17.8
|
1.0
|
O
|
B:HOH762
|
4.9
|
31.7
|
1.0
|
O
|
B:HOH388
|
4.9
|
19.2
|
1.0
|
|
Chlorine binding site 5 out
of 6 in 3rpl
Go back to
Chlorine Binding Sites List in 3rpl
Chlorine binding site 5 out
of 6 in the D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate
 Mono view
 Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 5 of D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Cl357
b:58.9
occ:1.00
|
OD2
|
C:ASP129
|
3.5
|
45.9
|
1.0
|
O
|
C:ASP129
|
3.8
|
41.2
|
1.0
|
CG
|
C:ASP129
|
4.1
|
55.4
|
1.0
|
CZ
|
C:TYR131
|
4.1
|
33.5
|
1.0
|
C
|
C:PHE130
|
4.2
|
31.1
|
1.0
|
O
|
C:PHE130
|
4.2
|
35.3
|
1.0
|
CE2
|
C:TYR131
|
4.2
|
31.5
|
1.0
|
C
|
C:ASP129
|
4.2
|
38.6
|
1.0
|
CB
|
C:ALA222
|
4.2
|
20.8
|
1.0
|
CE1
|
C:TYR131
|
4.3
|
32.9
|
1.0
|
CD2
|
C:TYR131
|
4.4
|
36.7
|
1.0
|
N
|
C:TYR131
|
4.4
|
28.8
|
1.0
|
CD1
|
C:TYR131
|
4.4
|
30.1
|
1.0
|
OD1
|
C:ASP129
|
4.5
|
63.6
|
1.0
|
CG
|
C:TYR131
|
4.5
|
33.3
|
1.0
|
CA
|
C:PHE130
|
4.6
|
31.6
|
1.0
|
OH
|
C:TYR131
|
4.6
|
33.5
|
1.0
|
N
|
C:PHE130
|
4.6
|
32.1
|
1.0
|
CA
|
C:ALA222
|
4.8
|
24.4
|
1.0
|
CA
|
C:TYR131
|
4.9
|
27.9
|
1.0
|
CA
|
C:ASP129
|
5.0
|
38.2
|
1.0
|
|
Chlorine binding site 6 out
of 6 in 3rpl
Go back to
Chlorine Binding Sites List in 3rpl
Chlorine binding site 6 out
of 6 in the D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate
 Mono view
 Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 6 of D-Fructose 1,6-Bisphosphatase Class 2/Sedoheptulose 1,7-Bisphosphatase of Synechocystis Sp. Pcc 6803 in Complex with Fructose-1,6- Bisphosphate within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Cl353
b:46.5
occ:1.00
|
O
|
D:HOH716
|
3.0
|
33.5
|
1.0
|
OG
|
D:SER197
|
3.1
|
22.9
|
1.0
|
OD2
|
D:ASP198
|
3.6
|
17.1
|
1.0
|
CB
|
D:SER197
|
3.6
|
18.2
|
1.0
|
CD2
|
D:LEU104
|
4.0
|
19.3
|
1.0
|
OE1
|
D:GLN109
|
4.0
|
27.1
|
1.0
|
CB
|
D:ASN103
|
4.1
|
19.6
|
1.0
|
CB
|
D:ASP198
|
4.2
|
18.9
|
1.0
|
N
|
D:LEU104
|
4.2
|
18.3
|
1.0
|
C
|
D:ASN103
|
4.2
|
19.4
|
1.0
|
CG
|
D:ASP198
|
4.3
|
23.6
|
1.0
|
O
|
D:SER197
|
4.3
|
20.2
|
1.0
|
O
|
D:ASN103
|
4.3
|
17.0
|
1.0
|
CG
|
D:LEU104
|
4.4
|
17.6
|
1.0
|
C
|
D:SER197
|
4.4
|
19.5
|
1.0
|
CA
|
D:LEU104
|
4.5
|
18.0
|
1.0
|
CA
|
D:SER197
|
4.7
|
16.5
|
1.0
|
ND2
|
D:ASN103
|
4.7
|
33.2
|
1.0
|
CG
|
D:ASN103
|
4.7
|
27.6
|
1.0
|
O
|
D:HOH392
|
4.8
|
22.0
|
1.0
|
O
|
D:HOH497
|
4.8
|
22.8
|
1.0
|
CA
|
D:ASN103
|
4.8
|
18.2
|
1.0
|
N
|
D:ASP198
|
4.8
|
18.0
|
1.0
|
|
Reference:
X.Hu,
F.Lingling.
New Insights Into the Structural and Interactional Basis For A Promising Route Towards Fructose-1,6-/Sedoheptulose-1,7-Bisphosphatases Controlling To Be Published.
Page generated: Fri Jul 11 09:56:55 2025
|