Chlorine in PDB 3sb6: Cu-Mediated Dimer of T4 Lysozyme D61H/K65H/R76H/R80H By Synthetic Symmetrization

All present enzymatic activity of Cu-Mediated Dimer of T4 Lysozyme D61H/K65H/R76H/R80H By Synthetic Symmetrization:
3.2.1.17;

The structure of Cu-Mediated Dimer of T4 Lysozyme D61H/K65H/R76H/R80H By Synthetic Symmetrization, PDB code: 3sb6 was solved by A.B.Soriaga, A.Laganowsky, M.Zhao, M.R.Sawaya, D.Cascio, T.O.Yeates, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	60.040, 69.840, 89.040, 90.00, 90.00, 90.00
R / Rfree (%)	22.6 / 27.2

The structure of Cu-Mediated Dimer of T4 Lysozyme D61H/K65H/R76H/R80H By Synthetic Symmetrization also contains other interesting chemical elements:

Copper

(Cu)

2 atoms

The binding sites of Chlorine atom in the Cu-Mediated Dimer of T4 Lysozyme D61H/K65H/R76H/R80H By Synthetic Symmetrization (pdb code 3sb6). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Cu-Mediated Dimer of T4 Lysozyme D61H/K65H/R76H/R80H By Synthetic Symmetrization, PDB code: 3sb6:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Cu-Mediated Dimer of T4 Lysozyme D61H/K65H/R76H/R80H By Synthetic Symmetrization


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Cu-Mediated Dimer of T4 Lysozyme D61H/K65H/R76H/R80H By Synthetic Symmetrization within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl164 b:83.3 occ:1.00 OG A:SER117 3.2 57.4 1.0 OD1 A:ASN132 3.4 70.2 1.0 CA A:PHE114 3.6 45.2 1.0 N A:SER117 3.7 47.1 1.0 C A:PHE114 3.7 48.9 1.0 CB A:SER117 3.8 47.7 1.0 O A:PHE114 3.9 48.4 1.0 N A:ASN116 4.1 49.4 1.0 CB A:ASN116 4.1 53.4 1.0 CB A:PHE114 4.1 45.4 1.0 CD2 A:PHE114 4.2 50.0 1.0 N A:THR115 4.3 45.7 1.0 CA A:SER117 4.4 45.4 1.0 OD1 A:ASN116 4.4 72.3 1.0 CA A:ASN116 4.4 50.4 1.0 C A:ASN116 4.6 52.4 1.0 CG A:PHE114 4.6 46.5 1.0 CG A:ASN132 4.6 77.2 1.0 O A:GLY113 4.7 51.4 1.0 CG A:ASN116 4.8 77.5 1.0 N A:PHE114 4.9 45.1 1.0 C A:THR115 4.9 51.0 1.0

Chlorine binding site 2 out of 2 in the Cu-Mediated Dimer of T4 Lysozyme D61H/K65H/R76H/R80H By Synthetic Symmetrization


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Cu-Mediated Dimer of T4 Lysozyme D61H/K65H/R76H/R80H By Synthetic Symmetrization within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl164 b:66.8 occ:1.00 O B:GLY30 3.5 41.1 1.0 N B:LEU32 3.7 39.3 1.0 CG B:ASP70 3.8 44.6 1.0 OD2 B:ASP70 3.9 47.8 1.0 CA B:HIS31 3.9 36.8 1.0 OD1 B:ASP70 4.0 45.5 1.0 O B:PHE104 4.1 48.8 1.0 CB B:ASP70 4.2 38.1 1.0 C B:HIS31 4.4 42.1 1.0 ND1 B:HIS31 4.4 43.6 1.0 C B:GLY30 4.5 40.9 1.0 CB B:LEU32 4.5 40.8 1.0 N B:HIS31 4.7 36.6 1.0 CA B:LEU32 4.8 40.3 1.0 CB B:HIS31 4.8 37.5 1.0 CG B:HIS31 4.9 41.7 1.0

A.Laganowsky, M.Zhao, A.B.Soriaga, M.R.Sawaya, D.Cascio, T.O.Yeates. An Approach to Crystallizing Proteins By Metal-Mediated Synthetic Symmetrization. Protein Sci. V. 20 1876 2011.
ISSN: ISSN 0961-8368
PubMed: 21898649
DOI: 10.1002/PRO.727