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Chlorine in PDB 3slp: Crystal Structure of Lambda Exonuclease in Complex with A 12 Bp Symmetric Dna Duplex

Enzymatic activity of Crystal Structure of Lambda Exonuclease in Complex with A 12 Bp Symmetric Dna Duplex

All present enzymatic activity of Crystal Structure of Lambda Exonuclease in Complex with A 12 Bp Symmetric Dna Duplex:
3.1.11.3;

Protein crystallography data

The structure of Crystal Structure of Lambda Exonuclease in Complex with A 12 Bp Symmetric Dna Duplex, PDB code: 3slp was solved by C.E.Bell, J.Zhang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 39.59 / 2.30
Space group P 65
Cell size a, b, c (Å), α, β, γ (°) 80.115, 80.115, 241.041, 90.00, 90.00, 120.00
R / Rfree (%) 21.1 / 28.1

Other elements in 3slp:

The structure of Crystal Structure of Lambda Exonuclease in Complex with A 12 Bp Symmetric Dna Duplex also contains other interesting chemical elements:

Calcium (Ca) 3 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of Lambda Exonuclease in Complex with A 12 Bp Symmetric Dna Duplex (pdb code 3slp). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Lambda Exonuclease in Complex with A 12 Bp Symmetric Dna Duplex, PDB code: 3slp:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 3slp

Go back to Chlorine Binding Sites List in 3slp
Chlorine binding site 1 out of 2 in the Crystal Structure of Lambda Exonuclease in Complex with A 12 Bp Symmetric Dna Duplex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Lambda Exonuclease in Complex with A 12 Bp Symmetric Dna Duplex within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl228

b:91.5
occ:1.00
CB B:ALA77 3.8 29.8 1.0
N B:ALA77 3.9 30.5 1.0
OP1 E:DG3 4.1 26.9 1.0
C5' E:DG3 4.1 35.3 1.0
CB B:LYS76 4.1 33.5 1.0
O3' E:DC2 4.1 31.5 1.0
N2 E:DG1 4.1 26.1 1.0
CA B:ALA77 4.4 29.9 1.0
CE2 B:PHE134 4.4 24.1 1.0
CD2 B:PHE134 4.5 24.7 1.0
N B:LYS76 4.5 33.6 1.0
P E:DG3 4.6 34.7 1.0
C1' E:DC2 4.6 27.9 1.0
CA B:LYS76 4.7 33.3 1.0
O5' E:DG3 4.7 32.9 1.0
C B:LYS76 4.8 31.8 1.0
O2 E:DC2 4.8 22.9 1.0

Chlorine binding site 2 out of 2 in 3slp

Go back to Chlorine Binding Sites List in 3slp
Chlorine binding site 2 out of 2 in the Crystal Structure of Lambda Exonuclease in Complex with A 12 Bp Symmetric Dna Duplex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Lambda Exonuclease in Complex with A 12 Bp Symmetric Dna Duplex within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl228

b:83.5
occ:1.00
NH2 C:ARG166 2.6 43.1 1.0
NH2 C:ARG108 2.9 41.5 1.0
CZ C:ARG108 2.9 40.4 1.0
NH1 C:ARG108 3.2 39.9 1.0
CZ C:ARG166 3.5 41.2 1.0
NE C:ARG108 3.5 36.9 1.0
NE C:ARG166 3.9 42.5 1.0
CD C:ARG108 4.3 35.1 1.0
CG C:ARG108 4.3 32.9 1.0
NH1 C:ARG166 4.4 35.4 1.0
O C:ARG108 4.7 34.2 1.0
O C:HOH309 4.9 32.3 1.0

Reference:

J.Zhang, K.A.Mccabe, C.E.Bell. Crystal Structures of {Lambda} Exonuclease in Complex with Dna Suggest An Electrostatic Ratchet Mechanism For Processivity. Proc.Natl.Acad.Sci.Usa V. 108 11872 2011.
ISSN: ISSN 0027-8424
PubMed: 21730170
DOI: 10.1073/PNAS.1103467108
Page generated: Fri Jul 11 10:21:46 2025

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