Chlorine in PDB 3wlv: Thermostable Urate Oxidase From Bacillus Sp. Tb-90

All present enzymatic activity of Thermostable Urate Oxidase From Bacillus Sp. Tb-90:
1.7.3.3;

The structure of Thermostable Urate Oxidase From Bacillus Sp. Tb-90, PDB code: 3wlv was solved by T.Hibi, Y.Hayashi, T.Itoh, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.92 / 1.75
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	133.571, 144.635, 70.794, 90.00, 90.00, 90.00
R / Rfree (%)	15.9 / 19

The structure of Thermostable Urate Oxidase From Bacillus Sp. Tb-90 also contains other interesting chemical elements:

Potassium

(K)

2 atoms

The binding sites of Chlorine atom in the Thermostable Urate Oxidase From Bacillus Sp. Tb-90 (pdb code 3wlv). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Thermostable Urate Oxidase From Bacillus Sp. Tb-90, PDB code: 3wlv:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Thermostable Urate Oxidase From Bacillus Sp. Tb-90


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Thermostable Urate Oxidase From Bacillus Sp. Tb-90 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl403 b:16.4 occ:1.00 OG1 B:THR73 3.1 13.9 1.0 ND2 A:ASN276 3.3 15.6 1.0 C5 A:AZA401 3.3 15.5 1.0 C4 A:AZA401 3.4 12.5 1.0 NE2 A:GLN304 3.4 16.1 1.0 CB A:ASN276 3.5 13.7 1.0 CA A:GLY302 3.5 14.0 1.0 C6 A:AZA401 3.5 17.7 1.0 N3 A:AZA401 3.7 12.4 1.0 N7 A:AZA401 3.7 16.6 1.0 CG2 B:THR73 3.8 13.3 1.0 N9 A:AZA401 3.8 15.7 1.0 N1 A:AZA401 3.8 14.8 1.0 CG A:ASN276 3.9 11.9 1.0 C2 A:AZA401 3.9 13.6 1.0 N8 A:AZA401 4.0 14.2 1.0 CB B:THR73 4.0 13.2 1.0 O6 A:AZA401 4.1 16.1 1.0 C A:GLY302 4.1 11.5 1.0 N A:GLY302 4.2 13.0 1.0 O B:HOH731 4.4 26.7 1.0 O A:HOH638 4.4 36.9 1.0 CD1 A:ILE249 4.5 18.8 1.0 N A:PHE303 4.6 11.7 1.0 CD A:GLN304 4.6 15.3 1.0 O A:GLY302 4.7 12.8 1.0 O2 A:AZA401 4.7 15.5 1.0 CA A:ASN276 4.9 12.5 1.0 OE1 A:GLN304 5.0 18.1 1.0 NZ B:LYS13 5.0 16.2 1.0

Chlorine binding site 2 out of 4 in the Thermostable Urate Oxidase From Bacillus Sp. Tb-90


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Thermostable Urate Oxidase From Bacillus Sp. Tb-90 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl404 b:16.5 occ:1.00 OG1 A:THR73 3.1 15.6 1.0 ND2 B:ASN276 3.2 21.6 1.0 C5 B:AZA401 3.3 15.4 1.0 NE2 B:GLN304 3.3 18.6 1.0 C4 B:AZA401 3.4 14.5 1.0 CB B:ASN276 3.5 15.3 1.0 CA B:GLY302 3.5 15.4 1.0 C6 B:AZA401 3.5 17.4 1.0 N7 B:AZA401 3.7 14.8 1.0 CG2 A:THR73 3.7 14.4 1.0 N3 B:AZA401 3.7 14.5 1.0 N9 B:AZA401 3.8 14.2 1.0 N1 B:AZA401 3.8 15.9 1.0 CG B:ASN276 3.9 16.0 1.0 C2 B:AZA401 4.0 16.0 1.0 N8 B:AZA401 4.0 13.9 1.0 CB A:THR73 4.0 14.2 1.0 O6 B:AZA401 4.1 15.7 1.0 C B:GLY302 4.1 15.2 1.0 N B:GLY302 4.2 13.6 1.0 CD1 B:ILE249 4.4 17.9 1.0 O B:HOH762 4.5 40.7 1.0 CD B:GLN304 4.5 18.7 1.0 N B:PHE303 4.6 13.9 1.0 O A:HOH512 4.6 23.0 1.0 O B:GLY302 4.6 12.6 1.0 O2 B:AZA401 4.8 17.4 1.0 CA B:ASN276 4.8 12.8 1.0 OE1 B:GLN304 4.9 16.2 1.0

Chlorine binding site 3 out of 4 in the Thermostable Urate Oxidase From Bacillus Sp. Tb-90


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Thermostable Urate Oxidase From Bacillus Sp. Tb-90 within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl403 b:17.4 occ:1.00 OG1 D:THR73 3.1 14.6 1.0 ND2 C:ASN276 3.3 19.3 1.0 C5 C:AZA401 3.3 13.1 1.0 NE2 C:GLN304 3.3 21.8 1.0 C4 C:AZA401 3.4 13.1 1.0 CB C:ASN276 3.5 14.8 1.0 CA C:GLY302 3.5 13.6 1.0 C6 C:AZA401 3.5 17.3 1.0 N3 C:AZA401 3.7 13.2 1.0 N7 C:AZA401 3.7 14.6 1.0 CG2 D:THR73 3.8 15.2 1.0 N1 C:AZA401 3.8 15.0 1.0 N9 C:AZA401 3.9 15.2 1.0 CG C:ASN276 3.9 17.3 1.0 C2 C:AZA401 3.9 12.5 1.0 CB D:THR73 4.0 16.1 1.0 N8 C:AZA401 4.0 15.1 1.0 O6 C:AZA401 4.1 15.4 1.0 C C:GLY302 4.1 12.9 1.0 N C:GLY302 4.2 12.7 1.0 O D:HOH715 4.4 25.2 1.0 CD1 C:ILE249 4.4 20.1 1.0 N C:PHE303 4.5 13.8 1.0 CD C:GLN304 4.5 18.3 1.0 O C:HOH727 4.6 37.4 1.0 O C:GLY302 4.6 12.9 1.0 O2 C:AZA401 4.7 16.2 1.0 CA C:ASN276 4.9 15.4 1.0 OE1 C:GLN304 4.9 17.4 1.0

Chlorine binding site 4 out of 4 in the Thermostable Urate Oxidase From Bacillus Sp. Tb-90


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Thermostable Urate Oxidase From Bacillus Sp. Tb-90 within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl402 b:18.1 occ:1.00 OG1 C:THR73 3.2 12.9 1.0 ND2 D:ASN276 3.2 16.8 1.0 C5 D:AZA401 3.3 14.2 1.0 NE2 D:GLN304 3.4 17.1 1.0 C4 D:AZA401 3.4 12.8 1.0 CB D:ASN276 3.4 12.7 1.0 CA D:GLY302 3.5 12.0 1.0 C6 D:AZA401 3.6 15.5 1.0 CG2 C:THR73 3.7 16.1 1.0 N3 D:AZA401 3.7 12.6 1.0 N7 D:AZA401 3.7 15.8 1.0 CG D:ASN276 3.8 15.2 1.0 N9 D:AZA401 3.8 16.1 1.0 N1 D:AZA401 3.8 13.6 1.0 C2 D:AZA401 3.9 13.0 1.0 CB C:THR73 4.0 13.0 1.0 N8 D:AZA401 4.0 14.8 1.0 C D:GLY302 4.1 10.8 1.0 O6 D:AZA401 4.1 14.2 1.0 N D:GLY302 4.2 14.1 1.0 O C:HOH720 4.3 27.4 1.0 CD1 D:ILE249 4.4 13.3 1.0 CD D:GLN304 4.5 16.9 1.0 N D:PHE303 4.5 11.4 1.0 O D:GLY302 4.6 13.1 1.0 O2 D:AZA401 4.7 14.2 1.0 CA D:ASN276 4.8 11.5 1.0 OE1 D:GLN304 4.9 18.1 1.0

T.Hibi, Y.Hayashi, H.Fukada, T.Itoh, T.Nago, Y.Nishiya. Intersubunit Salt Bridges with A Sulfate Anion Control Subunit Dissociation and Thermal Stabilization of Bacillus Sp. Tb-90 Urate Oxidase Biochemistry 2014.
ISSN: ISSN 0006-2960
PubMed: 24897238
DOI: 10.1021/BI500137B