Chlorine in PDB 3zxd: Wild-Type Lysenin

The structure of Wild-Type Lysenin, PDB code: 3zxd was solved by L.De Colibus, A.F.P.Sonnen, K.J.Morris, C.A.Siebert, P.Abrusci, J.Plitzko, V.Hodnik, M.Leippe, E.Volpi, G.Anderluh, R.J.C.Gilbert, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.09 / 3.30
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	58.910, 85.560, 108.810, 98.88, 96.84, 90.04
R / Rfree (%)	21.3 / 23.6

The structure of Wild-Type Lysenin also contains other interesting chemical elements:

Magnesium	(Mg)	6 atoms
Sodium	(Na)	6 atoms

The binding sites of Chlorine atom in the Wild-Type Lysenin (pdb code 3zxd). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Wild-Type Lysenin, PDB code: 3zxd:

Chlorine binding site 1 out of 1 in the Wild-Type Lysenin


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Wild-Type Lysenin within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl469 b:61.1 occ:1.00 O A:SER186 3.6 82.7 1.0 CB A:TRP187 3.7 72.6 1.0 CG A:TRP187 3.9 73.9 1.0 CE2 A:TYR221 4.0 62.0 1.0 CZ A:TYR221 4.1 67.4 1.0 CA A:TRP187 4.2 76.1 1.0 C A:SER186 4.3 83.4 1.0 CD2 A:TYR221 4.4 60.8 1.0 OH A:TYR221 4.4 70.8 1.0 CE1 A:TYR221 4.4 58.6 1.0 CD2 A:TRP187 4.4 71.6 1.0 CD1 A:TRP187 4.5 79.2 1.0 N A:TRP187 4.6 78.8 1.0 CD1 A:TYR221 4.7 56.7 1.0 CG A:TYR221 4.7 56.8 1.0 CE3 A:TRP187 4.8 70.0 1.0 CG A:TYR218 4.9 79.7 1.0 CD2 A:TYR218 5.0 84.2 1.0

L.De Colibus, A.F.P.Sonnen, K.J.Morris, C.A.Siebert, P.Abrusci, J.Plitzko, V.Hodnik, M.Leippe, E.Volpi, G.Anderluh, R.J.C.Gilbert. Structures of Lysenin Reveal A Shared Evolutionary Origin For Pore-Forming Proteins and Its Mode of Sphingomyelin Recognition. Structure V. 20 1498 2012.
ISSN: ISSN 0969-2126
PubMed: 22819216
DOI: 10.1016/J.STR.2012.06.011