Chlorine in PDB 4m4k: Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose

Enzymatic activity of Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose

All present enzymatic activity of Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose:
2.4.1.133;

Protein crystallography data

The structure of Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose, PDB code: 4m4k was solved by B.Ramakrishnan, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	36.98 / 2.20
*Space group*	P 4₃ 2₁ 2
*Cell size a, b, c (Å), α, β, γ (°)*	82.680, 82.680, 131.486, 90.00, 90.00, 90.00
*R / R_free (%)*	18.7 / 21.3

Other elements in 4m4k:

The structure of Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose also contains other interesting chemical elements:

Manganese

(Mn)

1 atom

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose (pdb code 4m4k). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose, PDB code: 4m4k:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 4m4k

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Chlorine Binding Sites List in 4m4k

Chlorine binding site 1 out of 2 in the Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose

Mono view

Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Cl405 b:71.3 occ:1.00	O	A:ARG263	3.8	56.0	1.0
	C	A:ARG263	4.3	53.6	1.0
	CA	A:GLU213	4.5	42.8	1.0
	N	A:GLU213	4.6	40.9	1.0
	O	A:ASP212	4.7	46.8	1.0
	C	A:ASP212	4.7	44.1	1.0
	CB	A:GLU213	4.7	37.7	1.0
	CA	A:ARG263	4.8	53.9	1.0
	CB	A:ASP212	4.8	42.2	1.0
	O	A:THR262	4.9	47.2	1.0
	N	A:LYS264	4.9	52.4	1.0
	CG2	A:VAL216	5.0	42.2	1.0
	CG	A:GLU213	5.0	40.7	1.0

Chlorine binding site 2 out of 2 in 4m4k

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Chlorine Binding Sites List in 4m4k

Chlorine binding site 2 out of 2 in the Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose

Mono view

Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the Drosphila Beta,14GALACTOSYLTRANSFERASE 7 Mutant D211N Complex with Manganese, Udp-Gal and Xylobiose within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Cl406 b:80.1 occ:1.00	O	A:HOH616	3.8	56.7	1.0
	NH1	A:ARG227	3.9	46.1	1.0
	CB	A:ARG227	4.0	48.8	1.0
	CD	A:ARG227	4.0	47.4	1.0
	CG	A:ARG227	4.1	46.8	1.0
	O	A:HOH600	4.1	52.5	1.0
	NE2	A:GLN229	4.2	62.6	1.0
	N	A:ARG227	4.5	50.3	1.0
	CA	A:ARG227	4.8	52.0	1.0
	CZ	A:ARG227	4.9	46.6	1.0
	NE	A:ARG227	4.9	46.7	1.0

Reference:

Y.Tsutsui, B.Ramakrishnan, P.K.Qasba. Crystal Structures of Beta-1,4-Galactosyltransferase 7 Enzyme Reveal Conformational Changes and Substrate Binding. J.Biol.Chem. V. 288 31963 2013.
ISSN: ISSN 0021-9258
PubMed: 24052259
DOI: 10.1074/JBC.M113.509984

Page generated: Fri Jul 11 18:55:42 2025

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