Chlorine in PDB 4tpw: The Co-Complex Structure of the Translation Initiation Factor EIF4E with the Inhibitor 4EGI-1 Reveals An Allosteric Mechanism For Dissociating EIF4G

The structure of The Co-Complex Structure of the Translation Initiation Factor EIF4E with the Inhibitor 4EGI-1 Reveals An Allosteric Mechanism For Dissociating EIF4G, PDB code: 4tpw was solved by E.Papadopoulos, S.Jenni, G.Wagner, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.67 / 1.50
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	39.340, 73.200, 65.720, 90.00, 106.25, 90.00
R / Rfree (%)	17.2 / 19.3

The binding sites of Chlorine atom in the The Co-Complex Structure of the Translation Initiation Factor EIF4E with the Inhibitor 4EGI-1 Reveals An Allosteric Mechanism For Dissociating EIF4G (pdb code 4tpw). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the The Co-Complex Structure of the Translation Initiation Factor EIF4E with the Inhibitor 4EGI-1 Reveals An Allosteric Mechanism For Dissociating EIF4G, PDB code: 4tpw:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the The Co-Complex Structure of the Translation Initiation Factor EIF4E with the Inhibitor 4EGI-1 Reveals An Allosteric Mechanism For Dissociating EIF4G


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of The Co-Complex Structure of the Translation Initiation Factor EIF4E with the Inhibitor 4EGI-1 Reveals An Allosteric Mechanism For Dissociating EIF4G within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:37.5 occ:1.00 CL19 A:33R302 0.0 37.5 1.0 C18 A:33R302 1.7 35.5 1.0 C17 A:33R302 2.7 33.9 1.0 C20 A:33R302 2.7 36.0 1.0 H171 A:33R302 2.8 40.6 1.0 HD2 A:HIS78 3.1 26.3 1.0 CL21 A:33R302 3.2 38.7 1.0 HG21 A:ILE63 3.2 32.1 1.0 HG12 A:ILE79 3.4 39.6 1.0 HE2 A:HIS78 3.5 30.3 1.0 HG23 A:ILE63 3.6 32.1 1.0 HD11 A:LEU75 3.7 27.0 1.0 CD2 A:HIS78 3.7 21.9 1.0 HD11 A:ILE79 3.8 38.9 1.0 HD11 A:ILE63 3.8 35.1 1.0 HG13 A:ILE79 3.8 39.6 1.0 CG2 A:ILE63 3.8 26.7 1.0 NE2 A:HIS78 3.9 25.3 1.0 C16 A:33R302 4.0 32.9 1.0 CG1 A:ILE79 4.0 33.0 1.0 C22 A:33R302 4.0 34.8 1.0 HD12 A:LEU75 4.1 27.0 1.0 HG22 A:ILE63 4.2 32.1 1.0 CD1 A:LEU75 4.3 22.5 1.0 CD1 A:ILE79 4.4 32.4 1.0 C15 A:33R302 4.5 33.3 1.0 HD13 A:LEU75 4.6 27.0 1.0 O A:HOH435 4.6 40.4 1.0 H161 A:33R302 4.7 39.5 1.0 CD1 A:ILE63 4.7 29.2 1.0 H221 A:33R302 4.8 41.8 1.0 HD13 A:ILE79 4.8 38.9 1.0 HD23 A:LEU45 4.9 27.0 1.0 HG12 A:ILE63 4.9 30.6 1.0 HE1 A:TYR91 4.9 33.9 1.0

Chlorine binding site 2 out of 2 in the The Co-Complex Structure of the Translation Initiation Factor EIF4E with the Inhibitor 4EGI-1 Reveals An Allosteric Mechanism For Dissociating EIF4G


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of The Co-Complex Structure of the Translation Initiation Factor EIF4E with the Inhibitor 4EGI-1 Reveals An Allosteric Mechanism For Dissociating EIF4G within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:38.7 occ:1.00 CL21 A:33R302 0.0 38.7 1.0 C20 A:33R302 1.7 36.0 1.0 C22 A:33R302 2.7 34.8 1.0 C18 A:33R302 2.7 35.5 1.0 HB2 A:SER83 2.8 52.0 1.0 H221 A:33R302 2.8 41.8 1.0 HG12 A:ILE79 3.1 39.6 1.0 CL19 A:33R302 3.2 37.5 1.0 HA A:ILE79 3.3 43.2 1.0 HE2 A:HIS78 3.4 30.3 1.0 HG A:SER83 3.5 52.9 1.0 CB A:SER83 3.6 43.3 1.0 HD2 A:HIS78 3.7 26.3 1.0 NE2 A:HIS78 3.7 25.3 1.0 OG A:SER83 3.9 44.1 1.0 CD2 A:HIS78 3.9 21.9 1.0 CG1 A:ILE79 3.9 33.0 1.0 C15 A:33R302 4.0 33.3 1.0 OG A:SER82 4.0 43.8 1.0 HG A:SER82 4.0 52.5 1.0 C17 A:33R302 4.0 33.9 1.0 HG23 A:ILE79 4.0 43.8 1.0 HB3 A:SER83 4.0 52.0 1.0 HG13 A:ILE79 4.1 39.6 1.0 CA A:ILE79 4.2 36.0 1.0 C16 A:33R302 4.5 32.9 1.0 OH A:TYR91 4.5 40.3 1.0 CB A:ILE79 4.5 35.0 1.0 HH A:TYR91 4.7 48.4 1.0 CE1 A:HIS78 4.7 28.9 1.0 O A:HIS78 4.7 22.2 1.0 CG2 A:ILE79 4.7 36.5 1.0 H171 A:33R302 4.8 40.6 1.0 CA A:SER83 4.8 46.5 1.0 N A:ILE79 4.8 35.1 1.0 N A:SER83 4.9 43.5 1.0 HA A:SER83 4.9 55.7 1.0 CG A:HIS78 4.9 20.7 1.0 O A:HOH419 5.0 39.9 1.0 HD11 A:ILE79 5.0 38.9 1.0

E.Papadopoulos, S.Jenni, E.Kabha, K.J.Takrouri, T.Yi, N.Salvi, R.E.Luna, E.Gavathiotis, P.Mahalingam, H.Arthanari, R.Rodriguez-Mias, R.Yefidoff-Freedman, B.H.Aktas, M.Chorev, J.A.Halperin, G.Wagner. Structure of the Eukaryotic Translation Initiation Factor EIF4E in Complex with 4EGI-1 Reveals An Allosteric Mechanism For Dissociating EIF4G. Proc.Natl.Acad.Sci.Usa V. 111 E3187 2014.
ISSN: ESSN 1091-6490
PubMed: 25049413
DOI: 10.1073/PNAS.1410250111