Chlorine in PDB 5lbe: Hif Prolyl Hydroxylase 2 (PHD2/EGLN1) G294E Variant in Complex with Mn(II) and N-[(1-Chloro-4-Hydroxyisoquinolin-3-Yl)Carbonyl]Glycine (IOX3/FG2216)

Enzymatic activity of Hif Prolyl Hydroxylase 2 (PHD2/EGLN1) G294E Variant in Complex with Mn(II) and N-[(1-Chloro-4-Hydroxyisoquinolin-3-Yl)Carbonyl]Glycine (IOX3/FG2216)

All present enzymatic activity of Hif Prolyl Hydroxylase 2 (PHD2/EGLN1) G294E Variant in Complex with Mn(II) and N-[(1-Chloro-4-Hydroxyisoquinolin-3-Yl)Carbonyl]Glycine (IOX3/FG2216):
1.14.11.29;

Protein crystallography data

The structure of Hif Prolyl Hydroxylase 2 (PHD2/EGLN1) G294E Variant in Complex with Mn(II) and N-[(1-Chloro-4-Hydroxyisoquinolin-3-Yl)Carbonyl]Glycine (IOX3/FG2216), PDB code: 5lbe was solved by R.Chowdhury, C.J.Schofield, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	32.21 / 1.75
*Space group*	P 6₃
*Cell size a, b, c (Å), α, β, γ (°)*	110.450, 110.450, 39.654, 90.00, 90.00, 120.00
*R / R_free (%)*	16.4 / 17.7

Other elements in 5lbe:

Manganese

(Mn)

1 atom

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Hif Prolyl Hydroxylase 2 (PHD2/EGLN1) G294E Variant in Complex with Mn(II) and N-[(1-Chloro-4-Hydroxyisoquinolin-3-Yl)Carbonyl]Glycine (IOX3/FG2216) (pdb code 5lbe). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Hif Prolyl Hydroxylase 2 (PHD2/EGLN1) G294E Variant in Complex with Mn(II) and N-[(1-Chloro-4-Hydroxyisoquinolin-3-Yl)Carbonyl]Glycine (IOX3/FG2216), PDB code: 5lbe:

Chlorine binding site 1 out of 1 in 5lbe

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Chlorine Binding Sites List in 5lbe

Chlorine binding site 1 out of 1 in the Hif Prolyl Hydroxylase 2 (PHD2/EGLN1) G294E Variant in Complex with Mn(II) and N-[(1-Chloro-4-Hydroxyisoquinolin-3-Yl)Carbonyl]Glycine (IOX3/FG2216)

Mono view

Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Hif Prolyl Hydroxylase 2 (PHD2/EGLN1) G294E Variant in Complex with Mn(II) and N-[(1-Chloro-4-Hydroxyisoquinolin-3-Yl)Carbonyl]Glycine (IOX3/FG2216) within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Cl502 b:33.9 occ:1.00	CL1	A:UN9502	0.0	33.9	1.0
	C7	A:UN9502	1.8	36.5	1.0
	H1	A:UN9502	2.6	38.1	1.0
	N8	A:UN9502	2.7	32.4	1.0
	C2	A:UN9502	2.8	29.0	1.0
	C1	A:UN9502	3.0	31.8	1.0
	MN	A:MN501	3.5	27.2	1.0
	HZ2	A:TRP389	3.5	39.8	1.0
	HD2	A:HIS313	3.5	34.8	1.0
	OD1	A:ASP315	3.5	25.7	1.0
	NE2	A:HIS313	3.6	26.3	1.0
	CD2	A:HIS313	3.8	29.0	1.0
	O	A:HOH623	3.9	40.8	1.0
	C9	A:UN9502	3.9	26.1	1.0
	HH	A:TYR310	3.9	43.9	1.0
	HA	A:ASP315	4.0	39.0	1.0
	C3	A:UN9502	4.0	26.6	1.0
	OH	A:TYR310	4.1	36.5	1.0
	CZ2	A:TRP389	4.3	33.1	1.0
	O	A:HOH612	4.3	25.5	1.0
	CG	A:ASP315	4.3	32.7	1.0
	HH2	A:TRP389	4.4	37.3	1.0
	C6	A:UN9502	4.4	36.0	1.0
	O	A:HOH633	4.4	57.4	1.0
	C10	A:UN9502	4.5	28.0	1.0
	CZ	A:TYR310	4.5	37.8	1.0
	HE1	A:TYR310	4.6	41.0	1.0
	CE1	A:HIS313	4.6	32.3	1.0
	HH12	A:ARG322	4.7	54.1	1.0
	CE1	A:TYR310	4.7	34.2	1.0
	CH2	A:TRP389	4.7	31.1	1.0
	HE1	A:MET299	4.8	39.5	1.0
	CA	A:ASP315	4.9	32.5	1.0
	H6	A:UN9502	4.9	43.3	1.0
	CG	A:HIS313	4.9	27.9	1.0
	C12	A:UN9502	5.0	25.1	1.0
	OD2	A:ASP315	5.0	29.4	1.0
	O13	A:UN9502	5.0	26.7	1.0

Reference:

R.Chowdhury, I.K.Leung, Y.M.Tian, M.I.Abboud, W.Ge, C.Domene, F.X.Cantrelle, I.Landrieu, A.P.Hardy, C.W.Pugh, P.J.Ratcliffe, T.D.Claridge, C.J.Schofield. Structural Basis For Oxygen Degradation Domain Selectivity of the Hif Prolyl Hydroxylases. Nat Commun V. 7 12673 2016.
ISSN: ESSN 2041-1723
PubMed: 27561929
DOI: 10.1038/NCOMMS12673

Page generated: Sat Jul 12 04:30:36 2025

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