Chlorine in PDB 5omb: Crystal Structure of K. Lactis DDC2 N-Terminus in Complex with S. Cerevisiae RFA1 N-Ob Domain

The structure of Crystal Structure of K. Lactis DDC2 N-Terminus in Complex with S. Cerevisiae RFA1 N-Ob Domain, PDB code: 5omb was solved by I.Deshpande, A.Seeber, K.Shimada, J.J.Keusch, H.Gut, S.M.Gasser, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.76 / 1.94
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	34.050, 94.940, 170.470, 90.00, 90.00, 90.00
R / Rfree (%)	18.5 / 22

The binding sites of Chlorine atom in the Crystal Structure of K. Lactis DDC2 N-Terminus in Complex with S. Cerevisiae RFA1 N-Ob Domain (pdb code 5omb). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Crystal Structure of K. Lactis DDC2 N-Terminus in Complex with S. Cerevisiae RFA1 N-Ob Domain, PDB code: 5omb:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Crystal Structure of K. Lactis DDC2 N-Terminus in Complex with S. Cerevisiae RFA1 N-Ob Domain


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of K. Lactis DDC2 N-Terminus in Complex with S. Cerevisiae RFA1 N-Ob Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl201 b:86.7 occ:1.00 O A:LEU47 3.5 45.0 1.0 C A:ILE48 4.1 38.1 1.0 CA A:ILE48 4.1 36.3 1.0 N A:MSE49 4.2 36.6 1.0 C A:LEU47 4.3 44.4 1.0 O A:ASN33 4.4 50.1 1.0 ND2 A:ASN33 4.4 93.4 1.0 N A:ILE48 4.6 36.5 1.0 CB A:MSE49 4.6 39.3 1.0 O A:ILE48 4.6 35.5 1.0 NZ A:LYS58 4.6 83.0 1.0 NE A:ARG35 4.6 0.3 1.0 CG A:ARG35 4.7 81.8 1.0 C A:ASN33 4.8 53.4 1.0 CA A:MSE49 4.9 35.8 1.0 CG A:LEU47 4.9 51.0 1.0

Chlorine binding site 2 out of 3 in the Crystal Structure of K. Lactis DDC2 N-Terminus in Complex with S. Cerevisiae RFA1 N-Ob Domain


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of K. Lactis DDC2 N-Terminus in Complex with S. Cerevisiae RFA1 N-Ob Domain within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl201 b:0.0 occ:1.00 N B:MSE49 4.0 37.1 1.0 C B:ILE48 4.1 39.0 1.0 O B:LEU47 4.1 43.4 1.0 CB B:MSE49 4.2 39.5 1.0 O B:ASN33 4.3 50.1 1.0 ND2 B:ASN33 4.3 73.2 1.0 CA B:ILE48 4.3 34.8 1.0 CE B:MSE49 4.5 51.3 1.0 O B:ILE48 4.5 37.4 1.0 CA B:MSE49 4.7 35.5 1.0 C B:ASN33 4.8 52.5 1.0 CB B:ASN33 4.8 50.8 1.0 C B:LEU47 4.8 40.4 1.0 N B:ILE48 4.9 33.1 1.0 CG B:ARG35 4.9 85.4 1.0

Chlorine binding site 3 out of 3 in the Crystal Structure of K. Lactis DDC2 N-Terminus in Complex with S. Cerevisiae RFA1 N-Ob Domain


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of K. Lactis DDC2 N-Terminus in Complex with S. Cerevisiae RFA1 N-Ob Domain within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl201 b:77.0 occ:1.00 CB D:LYS44 4.0 34.2 1.0 O D:LYS44 4.2 35.3 1.0 C D:LYS44 4.3 34.4 1.0 O D:HOH303 4.3 47.8 1.0 O D:HOH336 4.5 67.0 1.0 CE D:LYS44 4.6 42.1 1.0 N D:GLY45 4.6 32.0 1.0 CA D:LYS44 4.7 31.8 1.0 CA D:GLY45 5.0 32.2 1.0

I.Deshpande, A.Seeber, K.Shimada, J.J.Keusch, H.Gut, S.M.Gasser. Structural Basis of MEC1-DDC2-Rpa Assembly and Activation on Single-Stranded Dna at Sites of Damage. Mol. Cell V. 68 431 2017.
ISSN: ISSN 1097-4164
PubMed: 29033322
DOI: 10.1016/J.MOLCEL.2017.09.019