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Chlorine in PDB 6dot: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C, PDB code: 6dot was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 23.35 / 1.42
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.557, 37.229, 62.052, 90.00, 96.61, 90.00
R / Rfree (%) 14.2 / 16.8

Other elements in 6dot:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C also contains other interesting chemical elements:

Rubidium (Rb) 3 atoms
Magnesium (Mg) 2 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C (pdb code 6dot). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C, PDB code: 6dot:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in 6dot

Go back to Chlorine Binding Sites List in 6dot
Chlorine binding site 1 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl205

b:19.4
occ:0.75
HH A:TYR83 2.3 25.6 1.0
HD22 A:LEU111 3.0 19.6 1.0
HB3 A:LEU111 3.1 19.3 1.0
OH A:TYR83 3.1 21.4 1.0
O A:HOH375 3.1 23.0 1.0
HE2 A:TYR83 3.2 22.8 1.0
HD22 A:LEU161 3.2 26.7 1.0
HD13 A:LEU111 3.2 21.3 1.0
HB3 A:LEU161 3.3 21.6 1.0
O A:HOH365 3.4 20.3 1.0
HD13 A:LEU161 3.4 26.2 1.0
HD3 A:ARG97 3.7 27.5 1.0
CD2 A:LEU111 3.9 16.3 1.0
CE2 A:TYR83 3.9 19.0 1.0
CZ A:TYR83 3.9 17.8 1.0
CB A:LEU111 4.0 16.1 1.0
C1 A:GOL211 4.1 46.0 1.0
CD1 A:LEU111 4.1 17.7 1.0
O1 A:GOL211 4.1 42.1 1.0
CD2 A:LEU161 4.1 22.3 1.0
CG A:LEU111 4.2 15.1 1.0
CB A:LEU161 4.2 18.0 1.0
CD1 A:LEU161 4.3 21.9 1.0
HH11 A:ARG97 4.3 35.6 1.0
CG A:LEU161 4.4 18.6 1.0
HD21 A:LEU111 4.4 19.6 1.0
HD23 A:LEU111 4.4 19.6 1.0
HB2 A:HIS115 4.4 18.7 1.0
HB2 A:LEU111 4.5 19.3 1.0
HG2 A:ARG97 4.6 22.4 1.0
HD11 A:LEU111 4.6 21.3 1.0
CD A:ARG97 4.6 22.9 1.0
HD23 A:LEU161 4.6 26.7 1.0
O A:HOH414 4.6 21.6 1.0
HD21 A:LEU161 4.7 26.7 1.0
HB2 A:LEU161 4.7 21.6 1.0
HD12 A:LEU111 4.7 21.3 1.0
HA A:LEU111 4.7 18.1 1.0
HD11 A:LEU161 4.8 26.2 1.0
O A:LEU111 4.8 14.4 1.0
O A:HOH435 4.8 30.4 1.0
NH1 A:ARG97 4.8 29.7 1.0
HD12 A:LEU161 4.9 26.2 1.0
CA A:LEU111 4.9 15.1 1.0
HD11 A:ILE100 4.9 28.0 1.0
HA A:LEU161 5.0 21.0 1.0
O A:HOH348 5.0 21.9 1.0

Chlorine binding site 2 out of 3 in 6dot

Go back to Chlorine Binding Sites List in 6dot
Chlorine binding site 2 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl206

b:36.1
occ:0.50
O A:HOH447 2.6 48.4 1.0
O A:HOH362 2.8 23.9 1.0
O A:HOH468 2.8 40.2 1.0
HA A:GLN134 3.1 25.9 1.0
HD12 A:ILE137 3.2 24.0 1.0
HB2 A:LYS180 3.2 24.1 1.0
HD3 A:LYS180 3.2 27.5 1.0
HG21 A:ILE137 3.2 23.7 1.0
HB A:ILE137 3.4 21.1 1.0
HD13 A:ILE137 3.5 24.0 1.0
HG3 A:GLN134 3.6 35.9 1.0
CD1 A:ILE137 3.8 20.0 1.0
CG2 A:ILE137 3.9 19.8 1.0
HG22 A:ILE137 4.0 23.7 1.0
CB A:ILE137 4.1 17.6 1.0
CA A:GLN134 4.1 21.6 1.0
CD A:LYS180 4.1 22.9 1.0
CB A:LYS180 4.1 20.1 1.0
HG2 A:LYS180 4.2 27.1 1.0
O A:HOH339 4.3 34.1 1.0
CG A:LYS180 4.4 22.6 1.0
O A:HOH456 4.4 36.7 1.0
CG A:GLN134 4.4 29.9 1.0
O A:HOH466 4.4 59.1 1.0
O A:GLN134 4.5 21.3 1.0
CG1 A:ILE137 4.5 17.8 1.0
HD11 A:ILE137 4.6 24.0 1.0
HB3 A:LYS180 4.6 24.1 1.0
O A:HOH432 4.6 28.3 1.0
HB2 A:GLN134 4.6 28.4 1.0
CB A:GLN134 4.6 23.7 1.0
HD2 A:LYS180 4.6 27.5 1.0
HG23 A:ILE137 4.8 23.7 1.0
C A:GLN134 4.8 20.9 1.0
HG2 A:GLN134 4.8 35.9 1.0
HE3 A:LYS180 4.8 29.4 1.0
O A:HOH467 4.9 33.1 1.0
HA A:LYS180 4.9 21.3 1.0
N A:GLN134 5.0 20.1 1.0
CE A:LYS180 5.0 24.5 1.0

Chlorine binding site 3 out of 3 in 6dot

Go back to Chlorine Binding Sites List in 6dot
Chlorine binding site 3 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl207

b:34.4
occ:0.75
O A:HOH477 2.7 28.0 0.8
O A:HOH471 2.9 27.3 1.0
HD11 A:LEU179 3.1 25.7 1.0
HG3 A:PRO128 3.2 24.2 1.0
HB3 A:PRO128 3.3 21.7 1.0
HD21 A:LEU179 3.6 24.2 1.0
CD1 A:ILE64 3.6 41.3 1.0
CG A:PRO177 3.7 20.2 1.0
CB A:PRO177 3.8 18.9 1.0
CG A:PRO128 4.0 20.2 1.0
CB A:PRO128 4.0 18.1 1.0
CD1 A:LEU179 4.0 21.4 1.0
O A:HOH478 4.1 54.0 1.0
O A:HOH480 4.3 34.7 1.0
HD3 A:PRO128 4.4 20.9 1.0
HB2 A:PRO128 4.4 21.7 1.0
HD13 A:LEU179 4.4 25.7 1.0
CD2 A:LEU179 4.5 20.2 1.0
HD12 A:LEU179 4.6 25.7 1.0
HG A:LEU179 4.7 23.0 1.0
CG A:LEU179 4.7 19.2 1.0
HG2 A:PRO128 4.7 24.2 1.0
CD A:PRO128 4.8 17.4 1.0
HD22 A:LEU179 4.8 24.2 1.0
CD A:PRO177 4.8 17.3 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Sat Jul 12 12:55:11 2025

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