Chlorine in PDB 6ik6: Crystal Structure of Tomato Beta-Galactosidase (Tbg) 4 with Beta-1,4- Galactobiose

All present enzymatic activity of Crystal Structure of Tomato Beta-Galactosidase (Tbg) 4 with Beta-1,4- Galactobiose:
3.2.1.23;

The structure of Crystal Structure of Tomato Beta-Galactosidase (Tbg) 4 with Beta-1,4- Galactobiose, PDB code: 6ik6 was solved by K.Matsuyama, S.Nakae, K.Igarashi, T.Tada, M.Ishimaru, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.57 / 2.79
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	94.032, 110.730, 162.141, 90.00, 90.00, 90.00
R / Rfree (%)	19.6 / 28

The binding sites of Chlorine atom in the Crystal Structure of Tomato Beta-Galactosidase (Tbg) 4 with Beta-1,4- Galactobiose (pdb code 6ik6). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Tomato Beta-Galactosidase (Tbg) 4 with Beta-1,4- Galactobiose, PDB code: 6ik6:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Tomato Beta-Galactosidase (Tbg) 4 with Beta-1,4- Galactobiose


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Tomato Beta-Galactosidase (Tbg) 4 with Beta-1,4- Galactobiose within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl804 b:40.0 occ:1.00 NH1 A:ARG112 2.9 42.8 1.0 NE2 A:HIS47 2.9 42.8 1.0 OG A:SER45 3.0 37.4 1.0 CD A:ARG112 3.5 43.4 1.0 CB A:SER45 3.6 39.9 1.0 CD2 A:HIS47 3.7 44.6 1.0 CG A:GLU72 3.7 42.5 1.0 CD2 A:TYR74 3.8 46.3 1.0 CA A:THR73 3.9 42.2 1.0 N A:TYR74 4.0 47.2 1.0 CE2 A:TYR74 4.0 47.8 1.0 OE1 A:GLU72 4.0 48.1 1.0 CZ A:ARG112 4.0 46.5 1.0 N A:THR73 4.0 41.7 1.0 C A:THR73 4.0 44.2 1.0 CE1 A:HIS47 4.1 42.3 1.0 CE2 A:TYR289 4.1 46.1 1.0 C A:GLU72 4.1 43.5 1.0 CD2 A:TYR289 4.2 42.5 1.0 NE A:ARG112 4.2 45.2 1.0 O A:GLU72 4.2 38.6 1.0 CD A:GLU72 4.3 49.6 1.0 CB A:GLU72 4.4 39.1 1.0 CG A:TYR74 4.4 48.9 1.0 CA A:SER45 4.5 42.4 1.0 CZ A:TYR74 4.6 49.8 1.0 CG A:ARG112 4.7 44.9 1.0 O A:THR73 4.7 48.6 1.0 CA A:TYR74 4.9 45.2 1.0 CA A:GLU72 4.9 43.8 1.0 CG A:HIS47 4.9 47.1 1.0 CB A:TYR74 5.0 46.2 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Tomato Beta-Galactosidase (Tbg) 4 with Beta-1,4- Galactobiose


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Tomato Beta-Galactosidase (Tbg) 4 with Beta-1,4- Galactobiose within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl805 b:40.9 occ:1.00 OG B:SER45 3.0 37.8 1.0 NE2 B:HIS47 3.2 40.5 1.0 NH2 B:ARG112 3.2 39.6 1.0 NE B:ARG112 3.3 39.4 1.0 OE2 B:GLU72 3.4 45.5 1.0 CG B:GLU72 3.6 37.9 1.0 CZ B:ARG112 3.7 36.6 1.0 CB B:SER45 3.8 35.7 1.0 CD2 B:HIS47 3.8 41.8 1.0 CA B:THR73 3.9 37.4 1.0 CD2 B:TYR289 3.9 38.1 1.0 CE2 B:TYR74 3.9 42.0 1.0 CE2 B:TYR289 3.9 40.9 1.0 CD2 B:TYR74 4.0 42.9 1.0 CD B:GLU72 4.0 44.4 1.0 N B:TYR74 4.0 39.9 1.0 C B:THR73 4.0 40.2 1.0 N B:THR73 4.1 35.4 1.0 C B:GLU72 4.2 38.7 1.0 O B:GLU72 4.2 41.5 1.0 CE1 B:HIS47 4.3 39.9 1.0 CZ B:TYR74 4.3 42.1 1.0 CG B:TYR74 4.3 42.0 1.0 CD B:ARG112 4.4 38.2 1.0 CB B:GLU72 4.5 34.6 1.0 CG B:ARG112 4.5 38.6 1.0 CE1 B:TYR74 4.6 39.0 1.0 CD1 B:TYR74 4.6 40.3 1.0 O B:THR73 4.7 39.7 1.0 CA B:SER45 4.7 36.2 1.0 OH B:TYR74 4.9 44.0 1.0 CA B:TYR74 5.0 40.3 1.0

K.Matsuyama, T.Kondo, S.Nakae, K.Igarashi, T.Sakamoto, T.Tada, M.Ishimaru. Mechanism For the Substrate Recognition By Glycoside Hydrolase Family 35 Tomato Beta-Galactosidase (Tbg) 4 To Be Published.