Chlorine in PDB 6j1b: Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, on-State

The structure of Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, on-State, PDB code: 6j1b was solved by R.Nakashima, H.Shinoda, T.Matsuda, T.Nagai, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.62 / 1.99
Space group	I 21 3
Cell size a, b, c (Å), α, β, γ (°)	161.492, 161.492, 161.492, 90.00, 90.00, 90.00
R / Rfree (%)	14.3 / 16

The binding sites of Chlorine atom in the Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, on-State (pdb code 6j1b). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, on-State, PDB code: 6j1b:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, on-State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, on-State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl303 b:23.1 occ:1.00 O A:HOH426 3.0 17.4 1.0 O A:HOH580 3.2 27.4 1.0 N A:TRP62 3.2 15.1 1.0 O A:HOH521 3.4 52.4 1.0 CD1 A:TRP62 3.8 20.2 1.0 CA A:SER61 3.8 16.7 1.0 CB A:TRP62 3.9 16.7 1.0 C A:SER61 4.0 16.7 1.0 CB A:LYS142 4.0 25.3 1.0 CA A:TRP62 4.1 16.2 1.0 OG A:SER61 4.2 17.4 1.0 CG A:LYS142 4.2 27.1 1.0 CG A:TRP62 4.2 16.2 1.0 O A:HOH559 4.3 36.8 1.0 CB A:SER61 4.5 17.4 1.0 O A:HOH459 4.5 27.9 1.0 O A:HOH468 4.7 23.9 1.0 CA A:LYS142 4.7 21.1 1.0 CD A:LYS142 4.7 33.1 1.0 N A:VAL63 4.7 14.9 1.0 O A:MET60 4.7 16.9 1.0 O A:LYS142 4.8 19.7 1.0 N A:SER61 4.9 18.0 1.0 NE1 A:TRP62 5.0 19.9 1.0

Chlorine binding site 2 out of 2 in the Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, on-State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, on-State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl304 b:27.4 occ:1.00 O A:HOH494 2.9 32.4 1.0 O A:HOH444 3.2 35.5 1.0 N A:LYS198 3.2 17.2 1.0 N A:THR84 3.2 19.9 1.0 OG1 A:THR84 3.6 26.5 1.0 CA A:PRO197 3.7 17.2 1.0 CA A:ILE83 3.8 18.7 1.0 C A:PRO197 3.9 18.5 1.0 CB A:THR84 3.9 26.3 1.0 C A:ILE83 4.0 21.7 1.0 CG A:LYS198 4.0 36.9 1.0 CB A:LYS198 4.0 22.9 1.0 CG2 A:ILE83 4.1 18.4 1.0 CB A:PRO197 4.1 17.2 1.0 CA A:LYS198 4.2 20.6 1.0 CA A:THR84 4.2 21.3 1.0 O A:ASP82 4.3 25.4 1.0 CB A:ILE83 4.5 17.5 1.0 CD A:LYS198 4.6 54.1 1.0 O A:HOH599 4.7 38.0 1.0 N A:ILE83 4.8 21.3 1.0 O A:GLN196 4.9 18.3 1.0 O A:LYS198 4.9 17.6 1.0 N A:PRO197 4.9 18.1 1.0 C A:ASP82 4.9 28.7 1.0

H.Shinoda, K.Lu, R.Nakashima, T.Wazawa, K.Noguchi, T.Matsuda, T.Nagai. Acid-Tolerant Reversibly Switchable Green Fluorescent Protein For Super-Resolution Imaging Under Acidic Conditions. Cell Chem Biol V. 26 1469 2019.
ISSN: ESSN 2451-9456
PubMed: 31422907
DOI: 10.1016/J.CHEMBIOL.2019.07.012