Chlorine in PDB 6mgj: Crystal Structure of the Catalytic Domain From GH74 Enzyme POGH74 From Paenibacillus Odorifer, Apoenzyme

The structure of Crystal Structure of the Catalytic Domain From GH74 Enzyme POGH74 From Paenibacillus Odorifer, Apoenzyme, PDB code: 6mgj was solved by P.J.Stogios, T.Skarina, B.Nocek, G.Arnal, H.Brumer, A.Savchenko, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.78 / 2.00
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	239.094, 226.674, 187.224, 90.00, 114.07, 90.00
R / Rfree (%)	14.9 / 17.7

The structure of Crystal Structure of the Catalytic Domain From GH74 Enzyme POGH74 From Paenibacillus Odorifer, Apoenzyme also contains other interesting chemical elements:

Magnesium

(Mg)

9 atoms

The binding sites of Chlorine atom in the Crystal Structure of the Catalytic Domain From GH74 Enzyme POGH74 From Paenibacillus Odorifer, Apoenzyme (pdb code 6mgj). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Crystal Structure of the Catalytic Domain From GH74 Enzyme POGH74 From Paenibacillus Odorifer, Apoenzyme, PDB code: 6mgj:

Chlorine binding site 1 out of 1 in the Crystal Structure of the Catalytic Domain From GH74 Enzyme POGH74 From Paenibacillus Odorifer, Apoenzyme


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the Catalytic Domain From GH74 Enzyme POGH74 From Paenibacillus Odorifer, Apoenzyme within 5.0Å range: probe atom residue distance (Å) B Occ F:Cl805 b:62.9 occ:1.00 OG F:SER589 3.3 30.4 1.0 N F:LYS584 3.5 28.5 1.0 O F:SER589 3.6 28.5 1.0 OG1 F:THR591 3.8 31.0 1.0 CA F:SER583 3.9 24.3 1.0 C F:SER589 4.0 28.4 1.0 O F:TYR582 4.0 26.9 1.0 C F:SER583 4.2 26.5 1.0 CB F:SER589 4.3 28.8 1.0 N F:TRP590 4.3 26.1 1.0 N F:THR591 4.4 25.4 1.0 CA F:TRP590 4.4 24.6 1.0 C F:TRP590 4.5 26.1 1.0 CA F:LYS584 4.5 30.9 1.0 CB F:THR591 4.5 29.4 1.0 OG1 F:THR585 4.5 27.9 1.0 CB F:LYS584 4.5 34.7 1.0 OG F:SER583 4.5 24.6 1.0 CG F:LYS584 4.6 39.7 1.0 CE2 F:TYR582 4.6 30.6 1.0 CG2 F:THR585 4.6 29.4 1.0 CB F:SER583 4.7 23.3 1.0 C F:TYR582 4.7 28.0 1.0 N F:SER583 4.7 25.5 1.0 CA F:SER589 4.7 28.1 1.0 N F:THR585 4.8 27.6 1.0 CD2 F:TYR582 4.9 27.9 1.0 C F:LYS584 5.0 31.1 1.0

G.Arnal, P.J.Stogios, J.Asohan, T.Skarina, A.Savchenko, H.Brumer. Structural Enzymology Reveals the Molecular Basis of Substrate Regiospecificity and Processivity of An Exemplar Bacterial Glycoside Hydrolase Family 74ENDO-Xyloglucanase. Biochem. J. V. 475 3963 2018.
ISSN: ESSN 1470-8728
PubMed: 30463871
DOI: 10.1042/BCJ20180763