Chlorine in PDB 6q40: A Secreted Lysm Effector of the Wheat Pathogen Zymoseptoria Tritici Protects the Fungal Hyphae Against Chitinase Hydrolysis Through Ligand-Dependent Polymerisation of Lysm Homodimers

The structure of A Secreted Lysm Effector of the Wheat Pathogen Zymoseptoria Tritici Protects the Fungal Hyphae Against Chitinase Hydrolysis Through Ligand-Dependent Polymerisation of Lysm Homodimers, PDB code: 6q40 was solved by J.R.Mesters, R.Saleem-Batcha, A.Sanchez-Vallet, B.P.H.J.Thomma, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.45 / 2.41
Space group	P 61 2 2
Cell size a, b, c (Å), α, β, γ (°)	119.390, 119.390, 157.670, 90.00, 90.00, 120.00
R / Rfree (%)	18 / 22

The binding sites of Chlorine atom in the A Secreted Lysm Effector of the Wheat Pathogen Zymoseptoria Tritici Protects the Fungal Hyphae Against Chitinase Hydrolysis Through Ligand-Dependent Polymerisation of Lysm Homodimers (pdb code 6q40). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the A Secreted Lysm Effector of the Wheat Pathogen Zymoseptoria Tritici Protects the Fungal Hyphae Against Chitinase Hydrolysis Through Ligand-Dependent Polymerisation of Lysm Homodimers, PDB code: 6q40:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the A Secreted Lysm Effector of the Wheat Pathogen Zymoseptoria Tritici Protects the Fungal Hyphae Against Chitinase Hydrolysis Through Ligand-Dependent Polymerisation of Lysm Homodimers


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of A Secreted Lysm Effector of the Wheat Pathogen Zymoseptoria Tritici Protects the Fungal Hyphae Against Chitinase Hydrolysis Through Ligand-Dependent Polymerisation of Lysm Homodimers within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl101 b:47.6 occ:1.00 O B:HOH230 3.0 44.8 1.0 O A:HOH242 3.1 40.3 1.0 N A:ASP12 3.4 32.0 1.0 CA A:PHE11 3.7 29.3 1.0 CB A:PHE11 3.7 33.3 1.0 NH2 B:ARG2 3.7 49.6 1.0 CA A:ALA49 3.8 31.1 1.0 O A:ARG48 3.8 34.8 1.0 CD1 A:PHE11 3.9 30.4 1.0 N A:ALA49 3.9 24.7 1.0 C A:ARG48 3.9 29.9 1.0 CG A:ARG48 3.9 55.7 1.0 CG A:ASP12 4.0 48.7 1.0 C A:PHE11 4.1 33.5 1.0 OD2 A:ASP12 4.2 61.4 1.0 CB A:ASP12 4.2 38.6 1.0 CG A:PHE11 4.3 32.7 1.0 CB A:ARG48 4.3 39.4 1.0 OD1 A:ASP12 4.4 52.5 1.0 CA A:ASP12 4.4 32.7 1.0 CB A:ALA49 4.5 28.2 1.0 CZ B:ARG2 4.7 53.8 1.0 CA A:ARG48 4.8 33.2 1.0 C A:ALA49 4.9 38.2 1.0 O A:GLN10 4.9 42.7 1.0

Chlorine binding site 2 out of 3 in the A Secreted Lysm Effector of the Wheat Pathogen Zymoseptoria Tritici Protects the Fungal Hyphae Against Chitinase Hydrolysis Through Ligand-Dependent Polymerisation of Lysm Homodimers


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of A Secreted Lysm Effector of the Wheat Pathogen Zymoseptoria Tritici Protects the Fungal Hyphae Against Chitinase Hydrolysis Through Ligand-Dependent Polymerisation of Lysm Homodimers within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl104 b:53.7 occ:1.00 O B:HOH225 3.1 42.2 0.9 N B:ASP12 3.3 42.1 1.0 CA B:PHE11 3.7 40.2 1.0 NH2 A:ARG2 3.7 48.6 1.0 CA B:ALA49 3.7 44.2 1.0 CB B:PHE11 3.8 38.3 1.0 N B:ALA49 3.9 39.2 1.0 CD1 B:PHE11 3.9 33.9 1.0 O B:ARG48 3.9 52.4 1.0 C B:ARG48 3.9 48.3 1.0 CG B:ASP12 3.9 55.7 1.0 C B:PHE11 4.1 41.0 1.0 OD1 B:ASP12 4.1 62.2 1.0 CB B:ASP12 4.2 48.1 1.0 OD2 B:ASP12 4.3 64.2 1.0 CG B:ARG48 4.3 62.2 1.0 CD B:ARG48 4.3 80.0 1.0 CG B:PHE11 4.3 43.4 1.0 CB B:ARG48 4.3 48.3 1.0 CA B:ASP12 4.4 42.0 1.0 CB B:ALA49 4.4 39.5 1.0 CZ A:ARG2 4.6 60.4 1.0 CA B:ARG48 4.8 45.3 1.0 C B:ALA49 4.9 48.0 1.0 O B:GLN10 5.0 45.2 1.0

Chlorine binding site 3 out of 3 in the A Secreted Lysm Effector of the Wheat Pathogen Zymoseptoria Tritici Protects the Fungal Hyphae Against Chitinase Hydrolysis Through Ligand-Dependent Polymerisation of Lysm Homodimers


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of A Secreted Lysm Effector of the Wheat Pathogen Zymoseptoria Tritici Protects the Fungal Hyphae Against Chitinase Hydrolysis Through Ligand-Dependent Polymerisation of Lysm Homodimers within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl101 b:66.6 occ:1.00 N C:ASP12 3.2 58.1 1.0 NH2 D:ARG2 3.4 89.3 1.0 CA C:PHE11 3.4 59.7 1.0 CB C:PHE11 3.5 58.9 1.0 C C:PHE11 3.8 63.1 1.0 CD1 C:PHE11 3.9 55.4 1.0 CG C:ASP12 4.0 86.9 1.0 OD1 C:ASP12 4.1 97.0 1.0 CG C:PHE11 4.2 60.5 1.0 CB C:ARG48 4.2 75.8 1.0 OD2 C:ASP12 4.3 94.3 1.0 CB C:ASP12 4.3 71.8 1.0 CA C:ALA49 4.3 54.9 1.0 CA C:ASP12 4.3 69.2 1.0 N C:ALA49 4.3 52.0 1.0 C C:ARG48 4.4 59.1 1.0 O C:ARG48 4.4 62.2 1.0 CZ D:ARG2 4.4 98.0 1.0 O C:GLN10 4.7 78.9 1.0 N C:PHE11 4.8 70.4 1.0 CD C:ARG48 4.8 97.2 1.0 CB C:ALA49 5.0 53.5 1.0

A.Sanchez-Vallet, L.Rodriguez-Moreno, D.-J.Valkenburg, R.Saleem-Batcha, S.Wawra, D.Croll, J.R.Mesters, B.P.H.J.Thomma. A Secreted Lysm Effector Protects Fungal Hyphae Through Chitin-Dependent Homodimer Polymerization Biorxiv 2019.
DOI: 10.1101/787820