Chlorine in PDB 6r8m: Complex of Rice Blast (Magnaporthe Oryzae) Effector Protein Avr-Pike with An Engineered Hma Domain of Pikp-1 From Rice (Oryza Sativa)

The structure of Complex of Rice Blast (Magnaporthe Oryzae) Effector Protein Avr-Pike with An Engineered Hma Domain of Pikp-1 From Rice (Oryza Sativa), PDB code: 6r8m was solved by J.C.De La Concepcion, M.Franceschetti, M.J.Banfield, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.50 / 1.85
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	66.456, 80.701, 105.577, 90.00, 90.00, 90.00
R / Rfree (%)	18.6 / 23

The binding sites of Chlorine atom in the Complex of Rice Blast (Magnaporthe Oryzae) Effector Protein Avr-Pike with An Engineered Hma Domain of Pikp-1 From Rice (Oryza Sativa) (pdb code 6r8m). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Complex of Rice Blast (Magnaporthe Oryzae) Effector Protein Avr-Pike with An Engineered Hma Domain of Pikp-1 From Rice (Oryza Sativa), PDB code: 6r8m:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Complex of Rice Blast (Magnaporthe Oryzae) Effector Protein Avr-Pike with An Engineered Hma Domain of Pikp-1 From Rice (Oryza Sativa)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Complex of Rice Blast (Magnaporthe Oryzae) Effector Protein Avr-Pike with An Engineered Hma Domain of Pikp-1 From Rice (Oryza Sativa) within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl202 b:29.2 occ:1.00 O B:HOH316 3.0 29.9 1.0 O C:HOH358 3.0 32.3 1.0 O C:HOH331 3.0 25.1 1.0 N C:TYR71 3.3 23.4 1.0 CB C:CYS70 3.6 23.2 1.0 CD2 C:TYR71 3.6 26.1 1.0 CA C:CYS70 3.6 22.9 1.0 CD1 C:TRP74 3.8 27.1 1.0 C C:CYS70 4.0 22.9 1.0 O C:LYS75 4.0 27.3 1.0 CB C:MET76 4.1 26.1 1.0 CB C:TYR71 4.1 25.4 1.0 CG C:TYR71 4.3 24.6 1.0 CA C:TYR71 4.3 26.2 1.0 SD C:MET76 4.3 29.6 1.0 CE2 C:TYR71 4.5 28.9 1.0 OG B:SER258 4.5 25.2 1.0 CG C:MET76 4.5 30.0 1.0 NE1 C:TRP74 4.6 30.4 1.0 CB B:SER258 4.6 25.9 1.0 O C:TYR71 4.7 23.7 1.0 C C:LYS75 4.7 28.4 1.0 O B:GLN259 4.8 30.6 1.0 NE2 B:GLN256 4.8 26.6 1.0 CG C:TRP74 4.8 29.6 1.0 NZ B:LYS190 4.9 27.5 0.7 N C:CYS70 5.0 23.5 1.0

Chlorine binding site 2 out of 2 in the Complex of Rice Blast (Magnaporthe Oryzae) Effector Protein Avr-Pike with An Engineered Hma Domain of Pikp-1 From Rice (Oryza Sativa)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Complex of Rice Blast (Magnaporthe Oryzae) Effector Protein Avr-Pike with An Engineered Hma Domain of Pikp-1 From Rice (Oryza Sativa) within 5.0Å range: probe atom residue distance (Å) B Occ G:Cl202 b:39.2 occ:1.00 O F:HOH320 3.1 33.0 1.0 O F:HOH319 3.1 31.4 1.0 O G:HOH329 3.1 39.3 1.0 N G:TYR71 3.2 40.5 1.0 CB G:CYS70 3.6 36.3 1.0 CA G:CYS70 3.6 36.2 1.0 CD2 G:TYR71 3.6 31.7 1.0 CD1 G:TRP74 3.8 41.1 1.0 C G:CYS70 3.9 40.7 1.0 O G:LYS75 4.1 33.0 1.0 CB G:MET76 4.1 34.0 1.0 CB G:TYR71 4.1 36.0 1.0 CA G:TYR71 4.3 39.7 1.0 CG G:TYR71 4.3 37.4 1.0 SD G:MET76 4.3 37.7 1.0 OG F:SER258 4.5 35.8 1.0 NE1 G:TRP74 4.5 42.8 1.0 CG G:MET76 4.5 33.6 1.0 CE2 G:TYR71 4.6 35.0 1.0 NZ F:LYS190 4.6 31.0 0.7 O G:TYR71 4.6 38.0 1.0 CB F:SER258 4.7 35.1 1.0 C G:LYS75 4.7 30.7 1.0 O F:GLN259 4.8 37.2 1.0 CG G:TRP74 4.8 42.4 1.0 NE2 F:GLN256 4.8 29.6 1.0 C G:TYR71 5.0 39.5 1.0 N G:CYS70 5.0 34.3 1.0

J.C.De La Concepcion, M.Franceschetti, D.Maclean, R.Terauchi, S.Kamoun, M.J.Banfield. Protein Engineering Expands the Effector Recognition Profile of A Rice Nlr Immune Receptor. Elife V. 8 2019.
ISSN: ESSN 2050-084X
PubMed: 31535976
DOI: 10.7554/ELIFE.47713