Chlorine in PDB 6rum: Crystal Structure of Gfp-Lama-G97 - A Gfp Enhancer Nanobody with Cpdhfr Insertion and Tmp and Nadph

All present enzymatic activity of Crystal Structure of Gfp-Lama-G97 - A Gfp Enhancer Nanobody with Cpdhfr Insertion and Tmp and Nadph:
1.5.1.3;

The structure of Crystal Structure of Gfp-Lama-G97 - A Gfp Enhancer Nanobody with Cpdhfr Insertion and Tmp and Nadph, PDB code: 6rum was solved by H.Farrants, M.Tarnawski, T.G.Mueller, S.Otsuka, J.Hiblot, B.Koch, M.Kueblbeck, H.-G.Kraeusslich, J.Ellenberg, K.Johnsson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.72 / 1.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	47.890, 56.580, 53.290, 90.00, 94.91, 90.00
R / Rfree (%)	18.3 / 22.8

The binding sites of Chlorine atom in the Crystal Structure of Gfp-Lama-G97 - A Gfp Enhancer Nanobody with Cpdhfr Insertion and Tmp and Nadph (pdb code 6rum). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Crystal Structure of Gfp-Lama-G97 - A Gfp Enhancer Nanobody with Cpdhfr Insertion and Tmp and Nadph, PDB code: 6rum:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Crystal Structure of Gfp-Lama-G97 - A Gfp Enhancer Nanobody with Cpdhfr Insertion and Tmp and Nadph


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Gfp-Lama-G97 - A Gfp Enhancer Nanobody with Cpdhfr Insertion and Tmp and Nadph within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl303 b:28.9 occ:1.00 NH1 A:ARG145 3.1 30.4 1.0 N A:TRP47 3.1 18.9 1.0 NH2 A:ARG145 3.2 27.6 1.0 CZ A:ARG145 3.6 25.4 1.0 O A:HOH435 3.7 25.3 1.0 O A:GLY125 3.7 27.2 1.0 CB A:TRP47 3.7 18.0 1.0 CA A:GLU46 3.7 18.9 1.0 O A:HOH543 3.9 42.2 1.0 C A:GLU46 3.9 19.0 1.0 CA A:TRP47 4.0 16.8 1.0 CB A:ARG126 4.1 27.6 1.0 CA A:ARG126 4.2 22.0 1.0 CB A:GLU46 4.3 25.3 1.0 CD A:PRO127 4.4 23.9 1.0 O A:ARG45 4.4 25.4 1.0 C A:GLY125 4.7 25.9 1.0 O A:TRP47 4.8 16.1 1.0 NE A:ARG145 4.9 27.1 1.0 N A:GLU46 4.9 18.4 1.0 N A:ARG126 4.9 25.3 1.0 C A:TRP47 4.9 17.8 1.0

Chlorine binding site 2 out of 3 in the Crystal Structure of Gfp-Lama-G97 - A Gfp Enhancer Nanobody with Cpdhfr Insertion and Tmp and Nadph


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Gfp-Lama-G97 - A Gfp Enhancer Nanobody with Cpdhfr Insertion and Tmp and Nadph within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl304 b:21.0 occ:1.00 N A:VAL152 3.3 16.5 1.0 N6A A:NDP302 3.4 16.5 1.0 O A:HOH449 3.5 26.3 1.0 CB A:SER151 3.8 21.1 1.0 CA A:SER151 3.9 17.2 1.0 CB A:VAL152 3.9 18.6 1.0 CG2 A:VAL152 3.9 19.6 1.0 C A:SER151 4.1 17.7 1.0 CA A:VAL152 4.2 17.1 1.0 C6A A:NDP302 4.5 15.7 1.0 N1A A:NDP302 4.6 15.9 1.0 OE1 A:GLN176 4.6 18.4 1.0 OG A:SER151 4.9 21.1 1.0 O A:HOH614 4.9 30.9 1.0

Chlorine binding site 3 out of 3 in the Crystal Structure of Gfp-Lama-G97 - A Gfp Enhancer Nanobody with Cpdhfr Insertion and Tmp and Nadph


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of Gfp-Lama-G97 - A Gfp Enhancer Nanobody with Cpdhfr Insertion and Tmp and Nadph within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl305 b:29.2 occ:1.00 O A:HOH479 2.8 28.3 1.0 O A:HOH568 3.0 30.9 1.0 O A:HOH433 3.1 25.5 1.0 N A:SER33 3.2 15.2 1.0 O A:HOH457 3.3 20.4 1.0 CB A:SER33 3.8 20.0 1.0 O A:HOH576 3.8 33.2 1.0 CA A:TYR32 3.8 17.1 1.0 CB A:PRO99 3.9 19.4 1.0 C A:TYR32 4.0 18.3 1.0 CA A:SER33 4.1 14.8 1.0 OG A:SER33 4.1 24.2 1.0 CB A:TYR32 4.1 17.7 1.0 CD2 A:TYR32 4.2 17.5 1.0 CA A:PRO99 4.2 16.4 1.0 O A:ASN95 4.3 19.2 1.0 CB A:SER52A 4.4 19.2 1.0 O A:SER33 4.5 20.8 1.0 CD1 A:LEU102 4.6 24.4 1.0 CG A:TYR32 4.6 17.7 1.0 CB A:LEU102 4.6 15.0 1.0 O A:PRO99 4.6 19.2 1.0 C A:SER33 4.8 19.1 1.0 O A:HOH645 4.9 26.4 1.0 OG A:SER52A 4.9 25.6 1.0 O A:HOH559 5.0 23.0 1.0 C A:PRO99 5.0 16.5 1.0 O A:ARG31 5.0 21.3 1.0

H.Farrants, M.Tarnawski, T.G.Mueller, S.Otsuka, J.Hiblot, B.Koch, M.Kueblbeck, H.-G.Kraeusslich, J.Ellenberg, K.Johnsson. Chemogenetic Control of Nanobodies To Be Published.