Chlorine in PDB 7ldu: Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221 Mutant

All present enzymatic activity of Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221 Mutant:
2.3.1.9;

The structure of Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221 Mutant, PDB code: 7ldu was solved by A.C.Marshall, J.B.Bruning, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.79 / 2.85
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	83.853, 79.152, 150.959, 90, 94, 90
R / Rfree (%)	24 / 28.2

The binding sites of Chlorine atom in the Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221 Mutant (pdb code 7ldu). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221 Mutant, PDB code: 7ldu:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221 Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221 Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl407 b:44.5 occ:1.00 O B:HOH514 2.6 24.6 1.0 OG A:SER109 3.1 49.5 1.0 ND2 A:ASN50 3.5 21.4 1.0 OD1 A:ASN50 3.8 21.1 1.0 CB A:SER109 3.8 46.9 1.0 CG A:ASN50 4.0 21.0 1.0 N A:SER109 4.2 48.3 1.0 O1 B:SO4401 4.4 90.5 1.0 CA A:SER109 4.7 48.0 1.0 C A:ALA108 4.8 36.4 1.0 O4 B:SO4401 4.8 74.9 1.0 CA A:ALA108 4.9 35.5 1.0

Chlorine binding site 2 out of 2 in the Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221 Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221 Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl405 b:35.8 occ:1.00 O A:HOH511 3.0 19.7 1.0 OG B:SER109 3.2 60.3 1.0 CB B:SER109 3.8 56.5 1.0 ND2 B:ASN50 3.9 38.2 1.0 OD1 B:ASN50 4.0 36.8 1.0 CG B:ASN50 4.3 34.5 1.0 O2 A:SO4402 4.3 67.8 1.0 O3 A:SO4402 4.4 60.0 1.0 N B:SER109 4.4 57.6 1.0 CA B:SER109 4.8 56.8 1.0 C B:ALA108 5.0 24.2 1.0

A.C.Marshall, J.B.Bruning. Engineering Potassium Activation Into Biosynthetic Thiolase. Biochem.J. V. 478 3047 2021.
ISSN: ESSN 1470-8728
PubMed: 34338286
DOI: 10.1042/BCJ20210455