Chlorine in PDB 7m0c: Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound Mismatched Dsb Substrate

All present enzymatic activity of Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound Mismatched Dsb Substrate:
2.7.7.7;

The structure of Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound Mismatched Dsb Substrate, PDB code: 7m0c was solved by A.M.Kaminski, K.Bebenek, L.C.Pedersen, T.A.Kunkel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.37 / 2.65
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	56.205, 60.023, 139.824, 90, 90, 90
R / Rfree (%)	18 / 22.9

The structure of Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound Mismatched Dsb Substrate also contains other interesting chemical elements:

Sodium	(Na)	2 atoms
Magnesium	(Mg)	2 atoms

The binding sites of Chlorine atom in the Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound Mismatched Dsb Substrate (pdb code 7m0c). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound Mismatched Dsb Substrate, PDB code: 7m0c:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound Mismatched Dsb Substrate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound Mismatched Dsb Substrate within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl605 b:42.7 occ:1.00 OG A:SER515 3.3 27.9 1.0 CB A:SER515 3.9 25.9 1.0 O A:LYS238 4.1 47.3 1.0 CG1 A:VAL240 4.1 33.0 1.0 NZ A:LYS281 4.1 38.5 1.0 N A:VAL240 4.1 36.7 1.0 CE3 A:TRP239 4.3 34.3 1.0 CA A:TRP239 4.4 36.9 1.0 CA A:SER515 4.4 24.0 1.0 CE A:LYS281 4.5 29.8 1.0 CD A:LYS281 4.6 32.0 1.0 CG2 A:VAL240 4.8 34.1 1.0 C A:TRP239 4.8 40.9 1.0 O A:HOH703 4.8 29.4 1.0 CZ3 A:TRP239 4.8 33.8 1.0 CB A:VAL240 4.9 37.6 1.0

Chlorine binding site 2 out of 2 in the Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound Mismatched Dsb Substrate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Post-Catalytic Nicked Complex of Dna Polymerase Lambda with Bound Mismatched Dsb Substrate within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl606 b:50.2 occ:1.00 N A:GLU556 3.5 30.1 1.0 CA A:THR555 3.6 25.6 1.0 CB A:THR555 3.7 35.4 1.0 C A:THR555 4.1 27.8 1.0 CD2 A:LEU503 4.1 28.2 1.0 CG A:GLU556 4.3 28.1 1.0 CG2 A:THR555 4.4 27.0 1.0 O A:PRO554 4.5 37.2 1.0 CB A:GLU556 4.5 32.6 1.0 CA A:GLU556 4.6 31.4 1.0 OE1 A:GLU556 4.6 29.5 1.0 CB A:ALA500 4.7 23.0 1.0 OG1 A:THR555 4.8 34.4 1.0 N A:THR555 4.8 28.4 1.0 CD A:GLU556 4.9 29.9 1.0 CA A:ALA500 5.0 30.4 1.0

A.M.Kaminski, K.Bebenek, T.A.Kunkel, L.C.Pedersen. Comprehensive Structural Survey of Dna Double-Strand Break Synapsis By Dna Polymerase Lambda To Be Published.